Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1045 and recA are separated by 286 nucleotides recA and Sama_1047 are separated by 11 nucleotides Sama_1047 and alaS are separated by 134 nucleotides
Sama_1045: Sama_1045 - DNA mismatch repair protein MutS (RefSeq), at 1,262,651 to 1,265,215
_1045
Sama_1046: recA - recombinase A (RefSeq), at 1,265,502 to 1,266,569
recA
Sama_1047: Sama_1047 - hypothetical protein (RefSeq), at 1,266,581 to 1,267,018
_1047
Sama_1048: alaS - alanyl-tRNA synthetase (RefSeq), at 1,267,153 to 1,269,855
alaS
Position (kb)
1265
1266
1267 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1264.588 kb on + strand, within Sama_1045 at 1264.588 kb on + strand, within Sama_1045 at 1264.589 kb on - strand, within Sama_1045 at 1264.589 kb on - strand, within Sama_1045 at 1264.589 kb on - strand, within Sama_1045 at 1264.634 kb on - strand, within Sama_1045 at 1264.634 kb on - strand, within Sama_1045 at 1264.640 kb on - strand, within Sama_1045 at 1264.640 kb on - strand, within Sama_1045 at 1264.640 kb on - strand, within Sama_1045 at 1264.671 kb on + strand, within Sama_1045 at 1264.671 kb on + strand, within Sama_1045 at 1264.671 kb on + strand, within Sama_1045 at 1264.671 kb on + strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.672 kb on - strand, within Sama_1045 at 1264.760 kb on + strand, within Sama_1045 at 1264.760 kb on + strand, within Sama_1045 at 1264.760 kb on + strand, within Sama_1045 at 1264.761 kb on - strand, within Sama_1045 at 1264.852 kb on + strand, within Sama_1045 at 1264.922 kb on + strand, within Sama_1045 at 1264.974 kb on + strand at 1264.975 kb on - strand at 1265.089 kb on - strand at 1265.089 kb on - strand at 1265.089 kb on - strand at 1265.089 kb on - strand at 1265.100 kb on + strand at 1265.101 kb on - strand at 1265.101 kb on - strand at 1265.101 kb on - strand at 1265.118 kb on + strand at 1265.131 kb on + strand at 1265.131 kb on + strand at 1265.160 kb on + strand at 1265.161 kb on - strand at 1265.161 kb on - strand at 1265.161 kb on - strand at 1265.179 kb on + strand at 1265.180 kb on - strand at 1265.259 kb on + strand at 1265.273 kb on - strand at 1265.295 kb on - strand at 1265.375 kb on + strand at 1265.375 kb on + strand at 1265.375 kb on + strand at 1265.376 kb on - strand at 1265.405 kb on + strand at 1265.407 kb on + strand at 1265.420 kb on + strand at 1265.420 kb on + strand at 1265.430 kb on + strand at 1265.430 kb on + strand at 1265.430 kb on + strand at 1265.430 kb on + strand at 1265.430 kb on + strand at 1265.430 kb on + strand at 1265.431 kb on - strand at 1265.431 kb on - strand at 1265.457 kb on + strand at 1265.519 kb on + strand at 1265.520 kb on - strand at 1265.718 kb on - strand, within recA at 1265.809 kb on + strand, within recA at 1265.809 kb on + strand, within recA at 1265.809 kb on + strand, within recA at 1265.809 kb on + strand, within recA at 1265.809 kb on + strand, within recA at 1265.809 kb on + strand, within recA at 1265.809 kb on + strand, within recA at 1265.810 kb on - strand, within recA at 1265.810 kb on - strand, within recA at 1265.810 kb on - strand, within recA at 1265.811 kb on + strand, within recA at 1265.811 kb on + strand, within recA at 1265.812 kb on - strand, within recA at 1265.812 kb on - strand, within recA at 1265.830 kb on + strand, within recA at 1265.830 kb on + strand, within recA at 1265.830 kb on + strand, within recA at 1265.831 kb on - strand, within recA at 1265.841 kb on - strand, within recA at 1265.995 kb on + strand, within recA at 1266.010 kb on + strand, within recA at 1266.032 kb on + strand, within recA at 1266.032 kb on + strand, within recA at 1266.032 kb on + strand, within recA at 1266.308 kb on + strand, within recA at 1266.340 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.381 kb on + strand, within recA at 1266.382 kb on - strand, within recA at 1266.382 kb on - strand, within recA at 1266.382 kb on - strand, within recA at 1266.383 kb on + strand, within recA at 1266.523 kb on + strand at 1266.523 kb on + strand at 1266.523 kb on + strand at 1266.523 kb on + strand at 1266.640 kb on + strand, within Sama_1047 at 1266.640 kb on + strand, within Sama_1047 at 1266.640 kb on + strand, within Sama_1047 at 1266.641 kb on - strand, within Sama_1047 at 1266.703 kb on + strand, within Sama_1047 at 1266.704 kb on - strand, within Sama_1047 at 1266.704 kb on - strand, within Sama_1047 at 1266.704 kb on - strand, within Sama_1047 at 1266.704 kb on - strand, within Sama_1047 at 1266.710 kb on + strand, within Sama_1047 at 1266.798 kb on + strand, within Sama_1047 at 1266.799 kb on - strand, within Sama_1047 at 1266.814 kb on + strand, within Sama_1047 at 1266.823 kb on + strand, within Sama_1047 at 1266.823 kb on + strand, within Sama_1047 at 1266.824 kb on - strand, within Sama_1047 at 1266.860 kb on + strand, within Sama_1047 at 1266.860 kb on + strand, within Sama_1047 at 1266.861 kb on - strand, within Sama_1047 at 1266.861 kb on - strand, within Sama_1047 at 1266.937 kb on + strand, within Sama_1047 at 1266.937 kb on + strand, within Sama_1047 at 1266.938 kb on - strand, within Sama_1047 at 1266.938 kb on - strand, within Sama_1047 at 1266.938 kb on - strand, within Sama_1047 at 1266.938 kb on - strand, within Sama_1047 at 1266.939 kb on + strand, within Sama_1047 at 1266.939 kb on + strand, within Sama_1047 at 1266.939 kb on + strand, within Sama_1047 at 1266.939 kb on + strand, within Sama_1047 at 1266.940 kb on - strand, within Sama_1047 at 1266.944 kb on + strand, within Sama_1047 at 1266.945 kb on - strand, within Sama_1047 at 1266.945 kb on - strand, within Sama_1047 at 1267.004 kb on + strand at 1267.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,264,588 + Sama_1045 0.76 -1.1 1,264,588 + Sama_1045 0.76 -1.1 1,264,589 - Sama_1045 0.76 -1.8 1,264,589 - Sama_1045 0.76 -1.6 1,264,589 - Sama_1045 0.76 +0.9 1,264,634 - Sama_1045 0.77 +1.1 1,264,634 - Sama_1045 0.77 -1.9 1,264,640 - Sama_1045 0.78 -1.1 1,264,640 - Sama_1045 0.78 -0.6 1,264,640 - Sama_1045 0.78 -1.4 1,264,671 + Sama_1045 0.79 -2.4 1,264,671 + Sama_1045 0.79 +1.8 1,264,671 + Sama_1045 0.79 -2.0 1,264,671 + Sama_1045 0.79 -1.6 1,264,672 - Sama_1045 0.79 -0.7 1,264,672 - Sama_1045 0.79 +0.9 1,264,672 - Sama_1045 0.79 +1.3 1,264,672 - Sama_1045 0.79 +1.5 1,264,672 - Sama_1045 0.79 +1.6 1,264,672 - Sama_1045 0.79 +1.1 1,264,672 - Sama_1045 0.79 -0.9 1,264,672 - Sama_1045 0.79 -0.8 1,264,672 - Sama_1045 0.79 -1.1 1,264,672 - Sama_1045 0.79 -1.6 1,264,760 + Sama_1045 0.82 +2.8 1,264,760 + Sama_1045 0.82 +0.5 1,264,760 + Sama_1045 0.82 -2.1 1,264,761 - Sama_1045 0.82 -0.4 1,264,852 + Sama_1045 0.86 -0.7 1,264,922 + Sama_1045 0.89 +1.3 1,264,974 + -1.4 1,264,975 - +2.4 1,265,089 - -0.8 1,265,089 - +2.0 1,265,089 - -1.8 1,265,089 - -2.6 1,265,100 + -1.1 1,265,101 - +1.3 1,265,101 - +2.0 1,265,101 - -0.6 1,265,118 + -1.4 1,265,131 + -1.6 1,265,131 + +0.2 1,265,160 + -1.8 1,265,161 - +1.8 1,265,161 - -1.4 1,265,161 - +1.0 1,265,179 + -1.0 1,265,180 - +1.5 1,265,259 + -1.6 1,265,273 - +1.7 1,265,295 - +2.2 1,265,375 + +0.4 1,265,375 + +1.8 1,265,375 + +3.5 1,265,376 - -1.1 1,265,405 + -2.2 1,265,407 + -1.1 1,265,420 + +0.2 1,265,420 + -0.4 1,265,430 + -1.1 1,265,430 + -3.1 1,265,430 + -0.6 1,265,430 + -0.5 1,265,430 + -2.6 1,265,430 + -2.5 1,265,431 - -1.3 1,265,431 - -2.0 1,265,457 + -0.5 1,265,519 + -1.1 1,265,520 - -2.0 1,265,718 - recA Sama_1046 0.20 -0.4 1,265,809 + recA Sama_1046 0.29 -0.8 1,265,809 + recA Sama_1046 0.29 -1.1 1,265,809 + recA Sama_1046 0.29 -2.4 1,265,809 + recA Sama_1046 0.29 -0.6 1,265,809 + recA Sama_1046 0.29 -0.8 1,265,809 + recA Sama_1046 0.29 -2.3 1,265,809 + recA Sama_1046 0.29 -2.3 1,265,810 - recA Sama_1046 0.29 -1.4 1,265,810 - recA Sama_1046 0.29 -0.4 1,265,810 - recA Sama_1046 0.29 -0.8 1,265,811 + recA Sama_1046 0.29 -0.4 1,265,811 + recA Sama_1046 0.29 -1.1 1,265,812 - recA Sama_1046 0.29 -1.1 1,265,812 - recA Sama_1046 0.29 -1.6 1,265,830 + recA Sama_1046 0.31 -1.4 1,265,830 + recA Sama_1046 0.31 -2.0 1,265,830 + recA Sama_1046 0.31 -2.0 1,265,831 - recA Sama_1046 0.31 -2.1 1,265,841 - recA Sama_1046 0.32 -2.9 1,265,995 + recA Sama_1046 0.46 -0.4 1,266,010 + recA Sama_1046 0.48 -1.4 1,266,032 + recA Sama_1046 0.50 -0.4 1,266,032 + recA Sama_1046 0.50 -1.1 1,266,032 + recA Sama_1046 0.50 -0.4 1,266,308 + recA Sama_1046 0.75 -2.1 1,266,340 + recA Sama_1046 0.78 -0.4 1,266,381 + recA Sama_1046 0.82 -0.8 1,266,381 + recA Sama_1046 0.82 +0.2 1,266,381 + recA Sama_1046 0.82 -1.4 1,266,381 + recA Sama_1046 0.82 -1.4 1,266,381 + recA Sama_1046 0.82 -1.1 1,266,381 + recA Sama_1046 0.82 -0.8 1,266,381 + recA Sama_1046 0.82 -2.0 1,266,381 + recA Sama_1046 0.82 -0.8 1,266,381 + recA Sama_1046 0.82 -1.4 1,266,381 + recA Sama_1046 0.82 -2.7 1,266,381 + recA Sama_1046 0.82 -0.8 1,266,381 + recA Sama_1046 0.82 +0.2 1,266,382 - recA Sama_1046 0.82 -1.1 1,266,382 - recA Sama_1046 0.82 +1.2 1,266,382 - recA Sama_1046 0.82 -1.6 1,266,383 + recA Sama_1046 0.82 -1.4 1,266,523 + -2.0 1,266,523 + -0.2 1,266,523 + +1.2 1,266,523 + -0.4 1,266,640 + Sama_1047 0.13 -0.0 1,266,640 + Sama_1047 0.13 -1.5 1,266,640 + Sama_1047 0.13 -1.4 1,266,641 - Sama_1047 0.14 -0.4 1,266,703 + Sama_1047 0.28 -0.4 1,266,704 - Sama_1047 0.28 -0.9 1,266,704 - Sama_1047 0.28 -1.0 1,266,704 - Sama_1047 0.28 -1.6 1,266,704 - Sama_1047 0.28 -3.9 1,266,710 + Sama_1047 0.29 +0.8 1,266,798 + Sama_1047 0.50 -0.1 1,266,799 - Sama_1047 0.50 -0.4 1,266,814 + Sama_1047 0.53 -1.0 1,266,823 + Sama_1047 0.55 +2.3 1,266,823 + Sama_1047 0.55 -1.1 1,266,824 - Sama_1047 0.55 -1.5 1,266,860 + Sama_1047 0.64 +1.7 1,266,860 + Sama_1047 0.64 -2.6 1,266,861 - Sama_1047 0.64 +0.2 1,266,861 - Sama_1047 0.64 +1.0 1,266,937 + Sama_1047 0.81 -0.2 1,266,937 + Sama_1047 0.81 +0.2 1,266,938 - Sama_1047 0.82 -1.4 1,266,938 - Sama_1047 0.82 +2.9 1,266,938 - Sama_1047 0.82 +0.2 1,266,938 - Sama_1047 0.82 -0.4 1,266,939 + Sama_1047 0.82 +1.5 1,266,939 + Sama_1047 0.82 -1.1 1,266,939 + Sama_1047 0.82 -0.1 1,266,939 + Sama_1047 0.82 +1.0 1,266,940 - Sama_1047 0.82 -0.9 1,266,944 + Sama_1047 0.83 -0.4 1,266,945 - Sama_1047 0.83 +1.2 1,266,945 - Sama_1047 0.83 +0.2 1,267,004 + -0.1 1,267,045 + -1.1
Or see this region's nucleotide sequence