Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0596 and Sama_0597 are separated by 453 nucleotides Sama_0597 and Sama_0598 are separated by 217 nucleotides Sama_0598 and Sama_0599 are separated by 122 nucleotides
Sama_0596: Sama_0596 - IS1004 transposase (RefSeq), at 747,054 to 747,500
_0596
Sama_0597: Sama_0597 - hypothetical protein (RefSeq), at 747,954 to 748,385
_0597
Sama_0598: Sama_0598 - inorganic pyrophosphatase (RefSeq), at 748,603 to 749,136
_0598
Sama_0599: Sama_0599 - fructose-1,6-bisphosphatase (RefSeq), at 749,259 to 750,221
_0599
Position (kb) 747
748
749 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 746.958 kb on - strand at 747.543 kb on + strand at 747.543 kb on + strand at 747.543 kb on + strand at 747.573 kb on - strand at 747.573 kb on - strand at 747.594 kb on - strand at 747.606 kb on + strand at 747.652 kb on - strand at 747.654 kb on + strand at 747.672 kb on - strand at 747.672 kb on - strand at 747.688 kb on - strand at 747.706 kb on + strand at 747.732 kb on + strand at 747.736 kb on - strand at 747.738 kb on + strand at 747.776 kb on + strand at 747.797 kb on + strand at 747.797 kb on + strand at 747.835 kb on + strand at 747.849 kb on + strand at 747.849 kb on + strand at 747.849 kb on + strand at 747.849 kb on + strand at 747.850 kb on - strand at 747.862 kb on + strand at 747.863 kb on - strand at 747.891 kb on + strand at 747.921 kb on - strand at 747.963 kb on - strand at 748.023 kb on - strand, within Sama_0597 at 748.023 kb on - strand, within Sama_0597 at 748.086 kb on - strand, within Sama_0597 at 748.173 kb on - strand, within Sama_0597 at 748.184 kb on + strand, within Sama_0597 at 748.253 kb on + strand, within Sama_0597 at 748.254 kb on - strand, within Sama_0597 at 748.260 kb on + strand, within Sama_0597 at 748.261 kb on - strand, within Sama_0597 at 748.263 kb on - strand, within Sama_0597 at 748.263 kb on - strand, within Sama_0597 at 748.304 kb on + strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.305 kb on - strand, within Sama_0597 at 748.307 kb on + strand, within Sama_0597 at 748.308 kb on - strand, within Sama_0597 at 748.344 kb on + strand at 748.344 kb on + strand at 748.345 kb on - strand at 748.381 kb on + strand at 748.382 kb on - strand at 748.382 kb on - strand at 748.389 kb on + strand at 748.502 kb on + strand at 748.502 kb on + strand at 748.543 kb on + strand at 748.604 kb on + strand at 748.605 kb on - strand at 748.605 kb on - strand at 749.191 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.194 kb on - strand at 749.196 kb on - strand at 749.248 kb on + strand at 749.248 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 746,958 - -0.2 747,543 + +2.1 747,543 + +0.3 747,543 + +0.9 747,573 - +3.0 747,573 - -1.9 747,594 - -3.1 747,606 + -1.2 747,652 - -1.1 747,654 + -2.2 747,672 - +1.2 747,672 - -2.8 747,688 - -0.6 747,706 + +1.3 747,732 + +0.5 747,736 - -2.1 747,738 + -3.9 747,776 + -1.2 747,797 + +0.4 747,797 + -3.1 747,835 + -0.8 747,849 + -0.9 747,849 + -0.8 747,849 + -2.1 747,849 + +0.9 747,850 - -1.4 747,862 + -1.6 747,863 - +0.5 747,891 + -0.4 747,921 - -0.9 747,963 - +2.1 748,023 - Sama_0597 0.16 -1.4 748,023 - Sama_0597 0.16 +0.9 748,086 - Sama_0597 0.31 +3.0 748,173 - Sama_0597 0.51 -0.0 748,184 + Sama_0597 0.53 +4.3 748,253 + Sama_0597 0.69 +1.3 748,254 - Sama_0597 0.69 +2.1 748,260 + Sama_0597 0.71 +0.8 748,261 - Sama_0597 0.71 +2.0 748,263 - Sama_0597 0.72 +2.3 748,263 - Sama_0597 0.72 +2.2 748,304 + Sama_0597 0.81 +4.7 748,305 - Sama_0597 0.81 +1.1 748,305 - Sama_0597 0.81 +2.3 748,305 - Sama_0597 0.81 +2.4 748,305 - Sama_0597 0.81 +1.4 748,305 - Sama_0597 0.81 +2.6 748,307 + Sama_0597 0.82 +2.6 748,308 - Sama_0597 0.82 +4.2 748,344 + +2.7 748,344 + +1.1 748,345 - -1.4 748,381 + -0.2 748,382 - +2.4 748,382 - -0.6 748,389 + -2.1 748,502 + +2.7 748,502 + +1.6 748,543 + -0.1 748,604 + +3.6 748,605 - +0.0 748,605 - -2.4 749,191 - +0.4 749,194 - -1.6 749,194 - -0.2 749,194 - -0.6 749,194 - -3.1 749,194 - -2.7 749,194 - +1.1 749,196 - +0.4 749,248 + +1.4 749,248 + -2.3
Or see this region's nucleotide sequence