Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceE and aceF are separated by 11 nucleotides aceF and Sama_0377 are separated by 170 nucleotides
Sama_0375: aceE - pyruvate dehydrogenase subunit E1 (RefSeq), at 453,823 to 456,519
aceE
Sama_0376: aceF - dihydrolipoamide acetyltransferase (RefSeq), at 456,531 to 458,459
aceF
Sama_0377: Sama_0377 - dihydrolipoamide dehydrogenase (RefSeq), at 458,630 to 460,060
_0377
Position (kb) 456
457
458
459 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 455.538 kb on + strand, within aceE at 455.538 kb on + strand, within aceE at 455.620 kb on + strand, within aceE at 455.620 kb on + strand, within aceE at 455.620 kb on + strand, within aceE at 455.621 kb on - strand, within aceE at 455.621 kb on - strand, within aceE at 455.626 kb on + strand, within aceE at 455.626 kb on + strand, within aceE at 455.626 kb on + strand, within aceE at 455.627 kb on - strand, within aceE at 455.627 kb on - strand, within aceE at 455.627 kb on - strand, within aceE at 455.629 kb on + strand, within aceE at 455.629 kb on + strand, within aceE at 455.629 kb on + strand, within aceE at 455.630 kb on - strand, within aceE at 455.725 kb on - strand, within aceE at 455.793 kb on + strand, within aceE at 455.794 kb on - strand, within aceE at 455.799 kb on + strand, within aceE at 455.800 kb on - strand, within aceE at 455.800 kb on - strand, within aceE at 455.825 kb on - strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.866 kb on + strand, within aceE at 455.867 kb on - strand, within aceE at 455.867 kb on - strand, within aceE at 455.867 kb on - strand, within aceE at 455.890 kb on + strand, within aceE at 455.890 kb on + strand, within aceE at 455.891 kb on - strand, within aceE at 455.917 kb on + strand, within aceE at 455.971 kb on + strand, within aceE at 455.971 kb on + strand, within aceE at 455.989 kb on - strand, within aceE at 456.010 kb on + strand, within aceE at 456.010 kb on + strand, within aceE at 456.010 kb on + strand, within aceE at 456.071 kb on + strand, within aceE at 456.071 kb on + strand, within aceE at 456.072 kb on - strand, within aceE at 456.072 kb on - strand, within aceE at 456.111 kb on - strand, within aceE at 456.111 kb on - strand, within aceE at 456.183 kb on + strand, within aceE at 456.183 kb on + strand, within aceE at 456.183 kb on + strand, within aceE at 456.184 kb on - strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.217 kb on + strand, within aceE at 456.218 kb on - strand, within aceE at 456.218 kb on - strand, within aceE at 456.218 kb on - strand, within aceE at 456.265 kb on + strand at 456.278 kb on - strand at 456.316 kb on + strand at 456.316 kb on + strand at 456.697 kb on - strand at 456.854 kb on - strand, within aceF at 456.854 kb on - strand, within aceF at 456.949 kb on + strand, within aceF at 456.949 kb on + strand, within aceF at 456.968 kb on + strand, within aceF at 457.029 kb on - strand, within aceF at 457.286 kb on + strand, within aceF at 457.287 kb on - strand, within aceF at 457.294 kb on + strand, within aceF at 457.320 kb on - strand, within aceF at 457.384 kb on + strand, within aceF at 457.496 kb on + strand, within aceF at 457.496 kb on + strand, within aceF at 457.599 kb on - strand, within aceF at 457.622 kb on - strand, within aceF at 457.641 kb on + strand, within aceF at 457.642 kb on - strand, within aceF at 457.820 kb on + strand, within aceF at 458.043 kb on + strand, within aceF at 458.044 kb on - strand, within aceF at 458.044 kb on - strand, within aceF at 458.124 kb on + strand, within aceF at 458.124 kb on + strand, within aceF at 458.124 kb on + strand, within aceF at 458.180 kb on + strand, within aceF at 458.210 kb on + strand, within aceF at 458.211 kb on - strand, within aceF at 458.415 kb on + strand at 458.416 kb on - strand at 458.416 kb on - strand at 458.429 kb on - strand at 458.440 kb on + strand at 458.440 kb on + strand at 458.464 kb on - strand at 458.479 kb on + strand at 458.479 kb on + strand at 458.480 kb on - strand at 458.493 kb on + strand at 458.493 kb on + strand at 458.494 kb on - strand at 458.519 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.535 kb on + strand at 458.549 kb on + strand at 458.549 kb on + strand at 458.558 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 455,538 + aceE Sama_0375 0.64 -1.5 455,538 + aceE Sama_0375 0.64 -0.8 455,620 + aceE Sama_0375 0.67 -0.1 455,620 + aceE Sama_0375 0.67 -0.8 455,620 + aceE Sama_0375 0.67 -0.8 455,621 - aceE Sama_0375 0.67 -1.7 455,621 - aceE Sama_0375 0.67 -0.5 455,626 + aceE Sama_0375 0.67 -1.7 455,626 + aceE Sama_0375 0.67 -1.7 455,626 + aceE Sama_0375 0.67 -0.7 455,627 - aceE Sama_0375 0.67 -1.7 455,627 - aceE Sama_0375 0.67 -0.5 455,627 - aceE Sama_0375 0.67 -1.3 455,629 + aceE Sama_0375 0.67 +0.9 455,629 + aceE Sama_0375 0.67 -1.8 455,629 + aceE Sama_0375 0.67 -1.3 455,630 - aceE Sama_0375 0.67 -1.7 455,725 - aceE Sama_0375 0.71 -1.8 455,793 + aceE Sama_0375 0.73 -1.3 455,794 - aceE Sama_0375 0.73 -0.1 455,799 + aceE Sama_0375 0.73 -0.5 455,800 - aceE Sama_0375 0.73 -1.5 455,800 - aceE Sama_0375 0.73 -2.0 455,825 - aceE Sama_0375 0.74 -0.8 455,866 + aceE Sama_0375 0.76 -1.1 455,866 + aceE Sama_0375 0.76 -1.7 455,866 + aceE Sama_0375 0.76 -1.1 455,866 + aceE Sama_0375 0.76 -1.8 455,866 + aceE Sama_0375 0.76 -2.2 455,866 + aceE Sama_0375 0.76 -1.8 455,867 - aceE Sama_0375 0.76 -0.5 455,867 - aceE Sama_0375 0.76 -2.1 455,867 - aceE Sama_0375 0.76 -0.5 455,890 + aceE Sama_0375 0.77 -2.4 455,890 + aceE Sama_0375 0.77 -2.5 455,891 - aceE Sama_0375 0.77 -2.0 455,917 + aceE Sama_0375 0.78 -1.1 455,971 + aceE Sama_0375 0.80 -1.3 455,971 + aceE Sama_0375 0.80 -0.8 455,989 - aceE Sama_0375 0.80 -0.5 456,010 + aceE Sama_0375 0.81 -0.8 456,010 + aceE Sama_0375 0.81 -1.8 456,010 + aceE Sama_0375 0.81 -1.5 456,071 + aceE Sama_0375 0.83 -1.1 456,071 + aceE Sama_0375 0.83 +0.5 456,072 - aceE Sama_0375 0.83 -0.8 456,072 - aceE Sama_0375 0.83 -1.1 456,111 - aceE Sama_0375 0.85 -1.3 456,111 - aceE Sama_0375 0.85 -0.1 456,183 + aceE Sama_0375 0.88 -1.5 456,183 + aceE Sama_0375 0.88 -2.5 456,183 + aceE Sama_0375 0.88 -1.7 456,184 - aceE Sama_0375 0.88 -1.3 456,217 + aceE Sama_0375 0.89 -2.1 456,217 + aceE Sama_0375 0.89 -0.8 456,217 + aceE Sama_0375 0.89 -2.1 456,217 + aceE Sama_0375 0.89 -2.8 456,217 + aceE Sama_0375 0.89 -1.3 456,217 + aceE Sama_0375 0.89 -1.5 456,218 - aceE Sama_0375 0.89 -0.1 456,218 - aceE Sama_0375 0.89 -0.8 456,218 - aceE Sama_0375 0.89 -0.8 456,265 + -2.4 456,278 - -1.1 456,316 + -1.5 456,316 + -2.0 456,697 - -1.8 456,854 - aceF Sama_0376 0.17 -2.4 456,854 - aceF Sama_0376 0.17 +0.7 456,949 + aceF Sama_0376 0.22 -1.8 456,949 + aceF Sama_0376 0.22 -0.8 456,968 + aceF Sama_0376 0.23 -0.5 457,029 - aceF Sama_0376 0.26 -0.8 457,286 + aceF Sama_0376 0.39 -0.8 457,287 - aceF Sama_0376 0.39 -2.6 457,294 + aceF Sama_0376 0.40 -1.1 457,320 - aceF Sama_0376 0.41 +0.5 457,384 + aceF Sama_0376 0.44 -0.3 457,496 + aceF Sama_0376 0.50 -0.5 457,496 + aceF Sama_0376 0.50 -1.3 457,599 - aceF Sama_0376 0.55 -2.0 457,622 - aceF Sama_0376 0.57 -0.1 457,641 + aceF Sama_0376 0.58 -0.5 457,642 - aceF Sama_0376 0.58 -0.8 457,820 + aceF Sama_0376 0.67 -1.3 458,043 + aceF Sama_0376 0.78 -1.3 458,044 - aceF Sama_0376 0.78 -0.5 458,044 - aceF Sama_0376 0.78 -0.8 458,124 + aceF Sama_0376 0.83 -1.8 458,124 + aceF Sama_0376 0.83 -0.5 458,124 + aceF Sama_0376 0.83 -0.5 458,180 + aceF Sama_0376 0.85 -1.5 458,210 + aceF Sama_0376 0.87 -2.7 458,211 - aceF Sama_0376 0.87 -0.8 458,415 + -2.2 458,416 - -1.1 458,416 - +1.5 458,429 - -0.8 458,440 + -0.8 458,440 + -1.8 458,464 - -0.1 458,479 + -0.5 458,479 + -1.9 458,480 - -2.6 458,493 + -1.6 458,493 + +0.3 458,494 - -1.4 458,519 + -2.5 458,535 + -2.1 458,535 + -2.5 458,535 + -0.1 458,535 + -1.8 458,535 + -2.8 458,549 + +2.3 458,549 + -3.2 458,558 + -3.5
Or see this region's nucleotide sequence