Experiment: m.b. Doxycycline hyclate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22800 and PGA1_c22810 are separated by 369 nucleotides PGA1_c22810 and PGA1_c22820 are separated by 64 nucleotides PGA1_c22820 and PGA1_c22830 overlap by 1 nucleotides PGA1_c22830 and PGA1_c22840 are separated by 7 nucleotides PGA1_c22840 and PGA1_c22850 overlap by 11 nucleotides
PGA1_c22800: PGA1_c22800 - glycine betaine-binding protein, at 2,364,431 to 2,365,402
_c22800
PGA1_c22810: PGA1_c22810 - hypothetical protein, at 2,365,772 to 2,366,098
_c22810
PGA1_c22820: PGA1_c22820 - lytic transglycosylase-like protein, at 2,366,163 to 2,366,741
_c22820
PGA1_c22830: PGA1_c22830 - putative type IV secretion system protein VirB2, at 2,366,741 to 2,367,031
_c22830
PGA1_c22840: PGA1_c22840 - putative type IV secretory pathway virB3 protein, at 2,367,039 to 2,367,317
_c22840
PGA1_c22850: PGA1_c22850 - type IV secretion system protein VirB, at 2,367,307 to 2,369,673
_c22850
Position (kb)
2366
2367 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2365.198 kb on + strand, within PGA1_c22800 at 2365.198 kb on + strand, within PGA1_c22800 at 2365.206 kb on - strand, within PGA1_c22800 at 2365.211 kb on - strand, within PGA1_c22800 at 2365.227 kb on - strand, within PGA1_c22800 at 2365.231 kb on + strand, within PGA1_c22800 at 2365.238 kb on + strand, within PGA1_c22800 at 2365.238 kb on + strand, within PGA1_c22800 at 2365.246 kb on - strand, within PGA1_c22800 at 2365.246 kb on - strand, within PGA1_c22800 at 2365.246 kb on - strand, within PGA1_c22800 at 2365.285 kb on + strand, within PGA1_c22800 at 2365.285 kb on + strand, within PGA1_c22800 at 2365.318 kb on + strand at 2365.318 kb on + strand at 2365.320 kb on + strand at 2365.330 kb on + strand at 2365.338 kb on - strand at 2365.374 kb on + strand at 2365.376 kb on + strand at 2365.553 kb on - strand at 2365.654 kb on + strand at 2365.712 kb on + strand at 2365.762 kb on + strand at 2365.791 kb on + strand at 2365.799 kb on - strand at 2365.840 kb on + strand, within PGA1_c22810 at 2365.861 kb on - strand, within PGA1_c22810 at 2365.883 kb on - strand, within PGA1_c22810 at 2365.889 kb on - strand, within PGA1_c22810 at 2365.966 kb on + strand, within PGA1_c22810 at 2365.966 kb on + strand, within PGA1_c22810 at 2366.148 kb on - strand at 2366.167 kb on - strand at 2366.188 kb on - strand at 2366.194 kb on - strand at 2366.224 kb on - strand, within PGA1_c22820 at 2366.245 kb on + strand, within PGA1_c22820 at 2366.253 kb on - strand, within PGA1_c22820 at 2366.253 kb on - strand, within PGA1_c22820 at 2366.276 kb on + strand, within PGA1_c22820 at 2366.368 kb on + strand, within PGA1_c22820 at 2366.368 kb on + strand, within PGA1_c22820 at 2366.376 kb on - strand, within PGA1_c22820 at 2366.405 kb on - strand, within PGA1_c22820 at 2366.560 kb on + strand, within PGA1_c22820 at 2366.560 kb on + strand, within PGA1_c22820 at 2366.574 kb on - strand, within PGA1_c22820 at 2366.629 kb on + strand, within PGA1_c22820 at 2366.646 kb on + strand, within PGA1_c22820 at 2366.702 kb on + strand at 2366.702 kb on + strand at 2366.710 kb on - strand at 2366.712 kb on - strand at 2366.726 kb on + strand at 2366.734 kb on - strand at 2366.791 kb on - strand, within PGA1_c22830 at 2366.794 kb on + strand, within PGA1_c22830 at 2366.805 kb on + strand, within PGA1_c22830 at 2366.813 kb on - strand, within PGA1_c22830 at 2366.813 kb on - strand, within PGA1_c22830 at 2366.824 kb on - strand, within PGA1_c22830 at 2366.884 kb on - strand, within PGA1_c22830 at 2366.884 kb on - strand, within PGA1_c22830 at 2366.884 kb on - strand, within PGA1_c22830 at 2366.911 kb on - strand, within PGA1_c22830 at 2366.966 kb on - strand, within PGA1_c22830 at 2366.996 kb on + strand, within PGA1_c22830 at 2367.061 kb on + strand at 2367.094 kb on - strand, within PGA1_c22840 at 2367.125 kb on + strand, within PGA1_c22840 at 2367.181 kb on - strand, within PGA1_c22840 at 2367.183 kb on + strand, within PGA1_c22840 at 2367.183 kb on + strand, within PGA1_c22840 at 2367.191 kb on - strand, within PGA1_c22840 at 2367.193 kb on + strand, within PGA1_c22840 at 2367.196 kb on - strand, within PGA1_c22840 at 2367.196 kb on - strand, within PGA1_c22840 at 2367.196 kb on - strand, within PGA1_c22840 at 2367.201 kb on - strand, within PGA1_c22840 at 2367.201 kb on - strand, within PGA1_c22840 at 2367.201 kb on - strand, within PGA1_c22840 at 2367.232 kb on + strand, within PGA1_c22840 at 2367.240 kb on - strand, within PGA1_c22840 at 2367.250 kb on + strand, within PGA1_c22840 at 2367.300 kb on - strand at 2367.302 kb on + strand at 2367.312 kb on - strand at 2367.379 kb on + strand at 2367.428 kb on + strand at 2367.490 kb on + strand at 2367.592 kb on - strand, within PGA1_c22850 at 2367.615 kb on - strand, within PGA1_c22850 at 2367.647 kb on + strand, within PGA1_c22850 at 2367.649 kb on + strand, within PGA1_c22850 at 2367.657 kb on - strand, within PGA1_c22850 at 2367.684 kb on + strand, within PGA1_c22850 at 2367.694 kb on + strand, within PGA1_c22850 at 2367.694 kb on - strand, within PGA1_c22850 at 2367.702 kb on - strand, within PGA1_c22850
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Doxycycline hyclate 0.002 mg/ml remove 2,365,198 + PGA1_c22800 0.79 +0.2 2,365,198 + PGA1_c22800 0.79 -0.3 2,365,206 - PGA1_c22800 0.80 -0.3 2,365,211 - PGA1_c22800 0.80 -0.7 2,365,227 - PGA1_c22800 0.82 +0.5 2,365,231 + PGA1_c22800 0.82 +0.3 2,365,238 + PGA1_c22800 0.83 -2.2 2,365,238 + PGA1_c22800 0.83 -0.5 2,365,246 - PGA1_c22800 0.84 -0.0 2,365,246 - PGA1_c22800 0.84 -0.4 2,365,246 - PGA1_c22800 0.84 -0.8 2,365,285 + PGA1_c22800 0.88 +0.5 2,365,285 + PGA1_c22800 0.88 -0.0 2,365,318 + +0.2 2,365,318 + -0.0 2,365,320 + -1.3 2,365,330 + +0.2 2,365,338 - -0.5 2,365,374 + -0.2 2,365,376 + +0.3 2,365,553 - +0.2 2,365,654 + +1.2 2,365,712 + -0.9 2,365,762 + -0.1 2,365,791 + -0.3 2,365,799 - +0.5 2,365,840 + PGA1_c22810 0.21 +0.5 2,365,861 - PGA1_c22810 0.27 -0.5 2,365,883 - PGA1_c22810 0.34 -0.8 2,365,889 - PGA1_c22810 0.36 +0.5 2,365,966 + PGA1_c22810 0.59 -0.3 2,365,966 + PGA1_c22810 0.59 -0.9 2,366,148 - -0.2 2,366,167 - -1.4 2,366,188 - -0.6 2,366,194 - -2.0 2,366,224 - PGA1_c22820 0.11 +0.5 2,366,245 + PGA1_c22820 0.14 -1.8 2,366,253 - PGA1_c22820 0.16 +0.5 2,366,253 - PGA1_c22820 0.16 +0.4 2,366,276 + PGA1_c22820 0.20 +2.7 2,366,368 + PGA1_c22820 0.35 -0.9 2,366,368 + PGA1_c22820 0.35 -0.2 2,366,376 - PGA1_c22820 0.37 +0.1 2,366,405 - PGA1_c22820 0.42 +1.2 2,366,560 + PGA1_c22820 0.69 -0.4 2,366,560 + PGA1_c22820 0.69 +1.6 2,366,574 - PGA1_c22820 0.71 -0.4 2,366,629 + PGA1_c22820 0.80 +0.2 2,366,646 + PGA1_c22820 0.83 +0.3 2,366,702 + +1.3 2,366,702 + -0.7 2,366,710 - -0.3 2,366,712 - +0.1 2,366,726 + -0.5 2,366,734 - -0.7 2,366,791 - PGA1_c22830 0.17 -0.7 2,366,794 + PGA1_c22830 0.18 +0.7 2,366,805 + PGA1_c22830 0.22 -0.3 2,366,813 - PGA1_c22830 0.25 +0.3 2,366,813 - PGA1_c22830 0.25 -0.4 2,366,824 - PGA1_c22830 0.29 +0.1 2,366,884 - PGA1_c22830 0.49 +0.2 2,366,884 - PGA1_c22830 0.49 -0.1 2,366,884 - PGA1_c22830 0.49 +0.7 2,366,911 - PGA1_c22830 0.58 +0.3 2,366,966 - PGA1_c22830 0.77 -0.3 2,366,996 + PGA1_c22830 0.88 -3.2 2,367,061 + +0.6 2,367,094 - PGA1_c22840 0.20 -0.3 2,367,125 + PGA1_c22840 0.31 +0.5 2,367,181 - PGA1_c22840 0.51 -0.1 2,367,183 + PGA1_c22840 0.52 +0.5 2,367,183 + PGA1_c22840 0.52 -1.0 2,367,191 - PGA1_c22840 0.54 +0.9 2,367,193 + PGA1_c22840 0.55 +0.1 2,367,196 - PGA1_c22840 0.56 +0.8 2,367,196 - PGA1_c22840 0.56 +0.2 2,367,196 - PGA1_c22840 0.56 +0.6 2,367,201 - PGA1_c22840 0.58 +0.4 2,367,201 - PGA1_c22840 0.58 +0.5 2,367,201 - PGA1_c22840 0.58 -1.2 2,367,232 + PGA1_c22840 0.69 +0.7 2,367,240 - PGA1_c22840 0.72 +0.1 2,367,250 + PGA1_c22840 0.76 -0.5 2,367,300 - -0.3 2,367,302 + +0.1 2,367,312 - -2.5 2,367,379 + +0.8 2,367,428 + -0.1 2,367,490 + +0.2 2,367,592 - PGA1_c22850 0.12 -0.1 2,367,615 - PGA1_c22850 0.13 -0.4 2,367,647 + PGA1_c22850 0.14 -0.3 2,367,649 + PGA1_c22850 0.14 +0.5 2,367,657 - PGA1_c22850 0.15 -0.0 2,367,684 + PGA1_c22850 0.16 -0.1 2,367,694 + PGA1_c22850 0.16 +0.5 2,367,694 - PGA1_c22850 0.16 +0.1 2,367,702 - PGA1_c22850 0.17 -0.1
Or see this region's nucleotide sequence