Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tgt and queA are separated by 152 nucleotides queA and Sama_2195 are separated by 485 nucleotides
Sama_2193: tgt - queuine tRNA-ribosyltransferase (RefSeq), at 2,651,342 to 2,652,466
tgt
Sama_2194: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (RefSeq), at 2,652,619 to 2,653,656
queA
Sama_2195: Sama_2195 - hypothetical protein (RefSeq), at 2,654,142 to 2,654,735
_2195
Position (kb)
2652
2653
2654 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2651.670 kb on - strand, within tgt at 2651.742 kb on + strand, within tgt at 2651.839 kb on - strand, within tgt at 2651.895 kb on + strand, within tgt at 2651.896 kb on - strand, within tgt at 2651.896 kb on - strand, within tgt at 2652.053 kb on + strand, within tgt at 2652.183 kb on - strand, within tgt at 2652.364 kb on - strand at 2652.368 kb on - strand at 2652.446 kb on - strand at 2652.448 kb on + strand at 2652.634 kb on - strand at 2652.696 kb on - strand at 2652.706 kb on + strand at 2652.707 kb on - strand at 2652.707 kb on - strand at 2652.791 kb on - strand, within queA at 2652.817 kb on - strand, within queA at 2652.817 kb on - strand, within queA at 2652.822 kb on + strand, within queA at 2652.823 kb on - strand, within queA at 2652.968 kb on - strand, within queA at 2653.012 kb on + strand, within queA at 2653.126 kb on - strand, within queA at 2653.157 kb on + strand, within queA at 2653.157 kb on + strand, within queA at 2653.157 kb on + strand, within queA at 2653.158 kb on - strand, within queA at 2653.158 kb on - strand, within queA at 2653.206 kb on + strand, within queA at 2653.207 kb on - strand, within queA at 2653.208 kb on + strand, within queA at 2653.209 kb on - strand, within queA at 2653.224 kb on + strand, within queA at 2653.260 kb on + strand, within queA at 2653.260 kb on + strand, within queA at 2653.261 kb on - strand, within queA at 2653.261 kb on - strand, within queA at 2653.404 kb on + strand, within queA at 2653.405 kb on - strand, within queA at 2653.405 kb on - strand, within queA at 2653.405 kb on - strand, within queA at 2653.405 kb on - strand, within queA at 2653.406 kb on - strand, within queA at 2653.479 kb on + strand, within queA at 2653.638 kb on + strand at 2653.638 kb on + strand at 2653.639 kb on - strand at 2653.639 kb on - strand at 2653.639 kb on - strand at 2653.648 kb on + strand at 2653.649 kb on - strand at 2653.658 kb on + strand at 2653.658 kb on + strand at 2653.658 kb on + strand at 2653.658 kb on + strand at 2653.658 kb on + strand at 2653.658 kb on + strand at 2653.658 kb on + strand at 2653.659 kb on - strand at 2653.659 kb on - strand at 2653.659 kb on - strand at 2653.660 kb on + strand at 2653.661 kb on - strand at 2653.661 kb on - strand at 2653.661 kb on - strand at 2653.661 kb on - strand at 2653.661 kb on - strand at 2653.661 kb on - strand at 2653.693 kb on + strand at 2653.742 kb on + strand at 2653.742 kb on + strand at 2653.743 kb on - strand at 2653.743 kb on - strand at 2653.743 kb on - strand at 2653.754 kb on + strand at 2653.755 kb on - strand at 2653.769 kb on + strand at 2653.769 kb on + strand at 2653.839 kb on + strand at 2653.839 kb on + strand at 2653.891 kb on + strand at 2653.891 kb on + strand at 2653.891 kb on + strand at 2653.891 kb on + strand at 2653.909 kb on + strand at 2653.909 kb on + strand at 2653.909 kb on + strand at 2653.909 kb on + strand at 2653.909 kb on + strand at 2653.927 kb on + strand at 2653.945 kb on + strand at 2653.945 kb on + strand at 2653.945 kb on + strand at 2654.003 kb on - strand at 2654.003 kb on - strand at 2654.037 kb on + strand at 2654.037 kb on + strand at 2654.038 kb on - strand at 2654.072 kb on + strand at 2654.072 kb on + strand at 2654.073 kb on - strand at 2654.076 kb on - strand at 2654.107 kb on + strand at 2654.107 kb on + strand at 2654.108 kb on - strand at 2654.141 kb on + strand at 2654.174 kb on + strand at 2654.175 kb on - strand at 2654.344 kb on + strand, within Sama_2195 at 2654.345 kb on - strand, within Sama_2195 at 2654.345 kb on - strand, within Sama_2195 at 2654.362 kb on + strand, within Sama_2195 at 2654.363 kb on - strand, within Sama_2195 at 2654.363 kb on - strand, within Sama_2195 at 2654.375 kb on - strand, within Sama_2195 at 2654.412 kb on + strand, within Sama_2195 at 2654.413 kb on - strand, within Sama_2195 at 2654.413 kb on - strand, within Sama_2195 at 2654.432 kb on + strand, within Sama_2195 at 2654.433 kb on - strand, within Sama_2195 at 2654.451 kb on + strand, within Sama_2195 at 2654.466 kb on - strand, within Sama_2195 at 2654.466 kb on - strand, within Sama_2195 at 2654.538 kb on + strand, within Sama_2195 at 2654.538 kb on + strand, within Sama_2195 at 2654.538 kb on + strand, within Sama_2195
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,651,670 - tgt Sama_2193 0.29 -0.6 2,651,742 + tgt Sama_2193 0.36 +1.4 2,651,839 - tgt Sama_2193 0.44 +1.4 2,651,895 + tgt Sama_2193 0.49 -0.6 2,651,896 - tgt Sama_2193 0.49 -0.6 2,651,896 - tgt Sama_2193 0.49 -1.1 2,652,053 + tgt Sama_2193 0.63 +0.4 2,652,183 - tgt Sama_2193 0.75 -2.2 2,652,364 - -1.5 2,652,368 - -2.3 2,652,446 - -0.9 2,652,448 + -0.2 2,652,634 - -0.6 2,652,696 - -0.6 2,652,706 + -2.2 2,652,707 - -1.6 2,652,707 - -2.9 2,652,791 - queA Sama_2194 0.17 -1.1 2,652,817 - queA Sama_2194 0.19 -2.1 2,652,817 - queA Sama_2194 0.19 -1.9 2,652,822 + queA Sama_2194 0.20 -3.2 2,652,823 - queA Sama_2194 0.20 -2.2 2,652,968 - queA Sama_2194 0.34 -0.6 2,653,012 + queA Sama_2194 0.38 +0.4 2,653,126 - queA Sama_2194 0.49 -1.2 2,653,157 + queA Sama_2194 0.52 -1.8 2,653,157 + queA Sama_2194 0.52 +1.0 2,653,157 + queA Sama_2194 0.52 +0.4 2,653,158 - queA Sama_2194 0.52 +1.0 2,653,158 - queA Sama_2194 0.52 +0.4 2,653,206 + queA Sama_2194 0.57 +0.4 2,653,207 - queA Sama_2194 0.57 -1.9 2,653,208 + queA Sama_2194 0.57 +1.8 2,653,209 - queA Sama_2194 0.57 -3.1 2,653,224 + queA Sama_2194 0.58 -0.2 2,653,260 + queA Sama_2194 0.62 -0.9 2,653,260 + queA Sama_2194 0.62 -2.1 2,653,261 - queA Sama_2194 0.62 -1.2 2,653,261 - queA Sama_2194 0.62 -2.4 2,653,404 + queA Sama_2194 0.76 -0.2 2,653,405 - queA Sama_2194 0.76 -1.8 2,653,405 - queA Sama_2194 0.76 +2.0 2,653,405 - queA Sama_2194 0.76 -0.9 2,653,405 - queA Sama_2194 0.76 -2.4 2,653,406 - queA Sama_2194 0.76 -1.8 2,653,479 + queA Sama_2194 0.83 -0.2 2,653,638 + +1.4 2,653,638 + -3.1 2,653,639 - +0.4 2,653,639 - -1.8 2,653,639 - -0.2 2,653,648 + -1.6 2,653,649 - -0.6 2,653,658 + -1.2 2,653,658 + -1.2 2,653,658 + -0.9 2,653,658 + -1.6 2,653,658 + -2.8 2,653,658 + -0.9 2,653,658 + -2.1 2,653,659 - -1.4 2,653,659 - -0.6 2,653,659 - -1.2 2,653,660 + +1.7 2,653,661 - -0.6 2,653,661 - -0.4 2,653,661 - -1.6 2,653,661 - -1.0 2,653,661 - +0.8 2,653,661 - -0.9 2,653,693 + -1.1 2,653,742 + +2.2 2,653,742 + -0.9 2,653,743 - +4.0 2,653,743 - +3.0 2,653,743 - +2.4 2,653,754 + +0.8 2,653,755 - +0.6 2,653,769 + -1.2 2,653,769 + -1.8 2,653,839 + -1.9 2,653,839 + +0.6 2,653,891 + -0.6 2,653,891 + -0.4 2,653,891 + -1.4 2,653,891 + +1.4 2,653,909 + +0.4 2,653,909 + -1.2 2,653,909 + -0.4 2,653,909 + +0.8 2,653,909 + +0.4 2,653,927 + +2.4 2,653,945 + +0.4 2,653,945 + +1.1 2,653,945 + -0.9 2,654,003 - +1.1 2,654,003 - -0.5 2,654,037 + -0.2 2,654,037 + -1.2 2,654,038 - -0.2 2,654,072 + -1.4 2,654,072 + +1.6 2,654,073 - -1.4 2,654,076 - -0.2 2,654,107 + +0.2 2,654,107 + +3.4 2,654,108 - -0.2 2,654,141 + +0.7 2,654,174 + +0.4 2,654,175 - +0.7 2,654,344 + Sama_2195 0.34 -1.2 2,654,345 - Sama_2195 0.34 -2.9 2,654,345 - Sama_2195 0.34 +2.6 2,654,362 + Sama_2195 0.37 +0.8 2,654,363 - Sama_2195 0.37 -0.4 2,654,363 - Sama_2195 0.37 +2.1 2,654,375 - Sama_2195 0.39 +1.4 2,654,412 + Sama_2195 0.45 +1.7 2,654,413 - Sama_2195 0.46 +0.4 2,654,413 - Sama_2195 0.46 +0.4 2,654,432 + Sama_2195 0.49 +0.1 2,654,433 - Sama_2195 0.49 +0.4 2,654,451 + Sama_2195 0.52 -0.4 2,654,466 - Sama_2195 0.55 +1.8 2,654,466 - Sama_2195 0.55 +1.4 2,654,538 + Sama_2195 0.67 -0.6 2,654,538 + Sama_2195 0.67 +0.7 2,654,538 + Sama_2195 0.67 -0.9
Or see this region's nucleotide sequence