Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0517 and Sama_0518 are separated by 171 nucleotides Sama_0518 and Sama_0519 are separated by 9 nucleotides Sama_0519 and Sama_0520 are separated by 143 nucleotides
Sama_0517: Sama_0517 - D-beta-hydroxybutyrate dehydrogenase (RefSeq), at 636,932 to 637,732
_0517
Sama_0518: Sama_0518 - 3-oxoacid CoA-transferase (RefSeq), at 637,904 to 638,665
_0518
Sama_0519: Sama_0519 - 3-oxoacid CoA-transferase (RefSeq), at 638,675 to 639,340
_0519
Sama_0520: Sama_0520 - putative polyhydroxyalkanoate depolymerase (RefSeq), at 639,484 to 640,431
_0520
Position (kb) 638
639
640 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 637.690 kb on + strand at 637.691 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.733 kb on + strand at 637.733 kb on + strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.867 kb on + strand at 637.867 kb on + strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.883 kb on - strand at 637.883 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.916 kb on - strand at 637.966 kb on + strand at 637.966 kb on + strand at 637.967 kb on - strand at 637.967 kb on - strand at 637.982 kb on + strand, within Sama_0518 at 637.982 kb on + strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 638.051 kb on - strand, within Sama_0518 at 638.135 kb on + strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.203 kb on + strand, within Sama_0518 at 638.299 kb on + strand, within Sama_0518 at 638.301 kb on + strand, within Sama_0518 at 638.313 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.441 kb on - strand, within Sama_0518 at 638.444 kb on + strand, within Sama_0518 at 638.445 kb on - strand, within Sama_0518 at 638.457 kb on + strand, within Sama_0518 at 638.458 kb on - strand, within Sama_0518 at 638.511 kb on + strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.585 kb on + strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.659 kb on + strand at 638.659 kb on + strand at 638.659 kb on + strand at 638.660 kb on - strand at 638.663 kb on + strand at 638.663 kb on + strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.732 kb on + strand at 638.733 kb on - strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.735 kb on + strand at 638.736 kb on - strand at 638.736 kb on - strand at 638.751 kb on + strand, within Sama_0519 at 638.752 kb on - strand, within Sama_0519 at 638.752 kb on - strand, within Sama_0519 at 638.773 kb on + strand, within Sama_0519 at 638.773 kb on + strand, within Sama_0519 at 638.774 kb on - strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.777 kb on + strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.778 kb on - strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.793 kb on + strand, within Sama_0519 at 638.794 kb on - strand, within Sama_0519 at 638.794 kb on - strand, within Sama_0519 at 638.873 kb on - strand, within Sama_0519 at 638.970 kb on + strand, within Sama_0519 at 638.970 kb on + strand, within Sama_0519 at 638.971 kb on - strand, within Sama_0519 at 638.971 kb on - strand, within Sama_0519 at 638.971 kb on - strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.020 kb on + strand, within Sama_0519 at 639.021 kb on - strand, within Sama_0519 at 639.021 kb on - strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.093 kb on + strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.094 kb on - strand, within Sama_0519 at 639.176 kb on - strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.271 kb on + strand, within Sama_0519 at 639.272 kb on - strand, within Sama_0519 at 639.272 kb on - strand, within Sama_0519 at 639.339 kb on - strand at 639.339 kb on - strand at 639.339 kb on - strand at 639.410 kb on + strand at 639.410 kb on + strand at 639.410 kb on + strand at 639.410 kb on + strand at 639.411 kb on - strand at 639.411 kb on - strand at 639.411 kb on - strand at 639.411 kb on - strand at 639.440 kb on - strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.460 kb on + strand at 639.461 kb on - strand at 639.461 kb on - strand at 639.461 kb on - strand at 639.461 kb on - strand at 639.483 kb on + strand at 639.484 kb on - strand at 639.484 kb on - strand at 639.527 kb on + strand at 639.528 kb on - strand at 639.528 kb on - strand at 639.528 kb on - strand at 639.534 kb on + strand at 639.534 kb on + strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.535 kb on - strand at 639.631 kb on + strand, within Sama_0520 at 639.631 kb on + strand, within Sama_0520 at 639.631 kb on + strand, within Sama_0520 at 639.631 kb on + strand, within Sama_0520 at 639.632 kb on - strand, within Sama_0520 at 639.788 kb on + strand, within Sama_0520 at 639.789 kb on - strand, within Sama_0520 at 639.823 kb on - strand, within Sama_0520 at 639.855 kb on + strand, within Sama_0520 at 639.867 kb on + strand, within Sama_0520 at 639.867 kb on + strand, within Sama_0520 at 639.868 kb on - strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.904 kb on + strand, within Sama_0520 at 639.905 kb on - strand, within Sama_0520 at 639.917 kb on + strand, within Sama_0520 at 639.917 kb on + strand, within Sama_0520 at 639.918 kb on - strand, within Sama_0520 at 639.918 kb on - strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.954 kb on + strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.955 kb on - strand, within Sama_0520 at 639.989 kb on + strand, within Sama_0520 at 640.003 kb on + strand, within Sama_0520 at 640.004 kb on - strand, within Sama_0520 at 640.097 kb on + strand, within Sama_0520 at 640.214 kb on + strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.215 kb on - strand, within Sama_0520 at 640.308 kb on + strand, within Sama_0520 at 640.309 kb on - strand, within Sama_0520 at 640.309 kb on - strand, within Sama_0520 at 640.311 kb on + strand, within Sama_0520 at 640.311 kb on + strand, within Sama_0520
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 637,690 + +0.7 637,691 - +1.8 637,710 - -0.6 637,710 - -0.3 637,710 - +1.3 637,710 - +0.4 637,733 + -0.0 637,733 + +2.7 637,734 - +0.9 637,734 - +0.8 637,734 - +0.7 637,762 + -2.9 637,762 + -2.4 637,762 + +4.4 637,762 + +1.2 637,762 + +1.9 637,762 + -0.0 637,762 + +0.4 637,762 + +0.7 637,762 + -2.9 637,762 + -0.3 637,763 - +1.3 637,763 - -1.6 637,763 - -0.6 637,763 - +0.0 637,763 - +0.3 637,763 - -0.9 637,763 - +1.4 637,763 - +2.0 637,763 - +1.2 637,763 - -1.0 637,763 - +1.6 637,763 - +1.9 637,867 + -0.9 637,867 + +0.4 637,868 - -0.2 637,868 - -1.3 637,868 - +0.2 637,868 - +0.6 637,868 - -0.0 637,879 + -1.2 637,879 + -1.2 637,879 + -1.9 637,879 + +0.3 637,879 + +2.3 637,879 + +0.7 637,879 + +0.3 637,879 + -2.3 637,879 + -1.8 637,879 + +1.0 637,880 - -0.9 637,880 - -3.0 637,880 - -0.9 637,880 - +1.4 637,880 - +1.7 637,880 - +0.6 637,880 - -0.2 637,880 - +0.8 637,880 - -2.1 637,880 - +0.4 637,882 + -0.9 637,882 + -1.1 637,882 + -0.2 637,882 + +0.4 637,883 - +0.4 637,883 - +1.5 637,888 - +0.4 637,888 - -0.8 637,888 - +2.6 637,888 - +0.2 637,888 - -0.9 637,888 - -1.4 637,912 + -1.6 637,912 + +2.0 637,912 + -1.1 637,912 + +3.7 637,912 + +1.2 637,913 - +0.2 637,913 - -0.1 637,913 - -0.9 637,915 + -0.7 637,915 + +0.2 637,915 + -1.3 637,915 + +0.5 637,916 - -2.1 637,966 + -0.3 637,966 + +0.4 637,967 - -1.1 637,967 - -0.1 637,982 + Sama_0518 0.10 -0.8 637,982 + Sama_0518 0.10 -0.3 637,983 - Sama_0518 0.10 +0.8 637,983 - Sama_0518 0.10 +2.2 637,983 - Sama_0518 0.10 -1.5 637,983 - Sama_0518 0.10 +1.8 638,051 - Sama_0518 0.19 -0.6 638,135 + Sama_0518 0.30 +0.6 638,136 - Sama_0518 0.30 +0.7 638,136 - Sama_0518 0.30 -2.5 638,189 + Sama_0518 0.37 +2.0 638,189 + Sama_0518 0.37 -0.9 638,189 + Sama_0518 0.37 -2.6 638,189 + Sama_0518 0.37 -0.7 638,189 + Sama_0518 0.37 +0.1 638,190 - Sama_0518 0.38 +1.3 638,190 - Sama_0518 0.38 -1.8 638,190 - Sama_0518 0.38 -0.8 638,190 - Sama_0518 0.38 +0.3 638,190 - Sama_0518 0.38 +1.2 638,190 - Sama_0518 0.38 -1.9 638,203 + Sama_0518 0.39 +0.2 638,299 + Sama_0518 0.52 +1.9 638,301 + Sama_0518 0.52 -1.2 638,313 - Sama_0518 0.54 -1.1 638,382 - Sama_0518 0.63 +1.4 638,382 - Sama_0518 0.63 -3.1 638,441 - Sama_0518 0.70 -0.4 638,444 + Sama_0518 0.71 -1.8 638,445 - Sama_0518 0.71 +1.8 638,457 + Sama_0518 0.73 +0.1 638,458 - Sama_0518 0.73 +0.5 638,511 + Sama_0518 0.80 +0.4 638,512 - Sama_0518 0.80 -0.4 638,512 - Sama_0518 0.80 -0.5 638,512 - Sama_0518 0.80 +1.5 638,512 - Sama_0518 0.80 -0.7 638,512 - Sama_0518 0.80 +2.3 638,585 + Sama_0518 0.89 +0.3 638,586 - Sama_0518 0.90 -2.3 638,586 - Sama_0518 0.90 -2.2 638,586 - Sama_0518 0.90 +0.3 638,659 + +1.6 638,659 + +1.1 638,659 + -0.1 638,660 - -0.2 638,663 + -1.4 638,663 + -0.6 638,664 - +1.2 638,664 - +1.8 638,664 - +1.9 638,732 + +0.8 638,733 - -1.4 638,735 + -1.2 638,735 + -3.1 638,735 + +1.1 638,735 + -3.2 638,735 + -0.4 638,735 + -0.3 638,735 + -1.2 638,736 - +0.2 638,736 - -1.8 638,751 + Sama_0519 0.11 +1.4 638,752 - Sama_0519 0.12 -0.2 638,752 - Sama_0519 0.12 -0.9 638,773 + Sama_0519 0.15 +0.2 638,773 + Sama_0519 0.15 +1.0 638,774 - Sama_0519 0.15 -0.6 638,777 + Sama_0519 0.15 +3.1 638,777 + Sama_0519 0.15 -2.3 638,777 + Sama_0519 0.15 +1.5 638,777 + Sama_0519 0.15 -0.9 638,777 + Sama_0519 0.15 -1.1 638,777 + Sama_0519 0.15 -1.1 638,778 - Sama_0519 0.15 +1.2 638,778 - Sama_0519 0.15 +0.2 638,778 - Sama_0519 0.15 -1.6 638,778 - Sama_0519 0.15 -0.3 638,778 - Sama_0519 0.15 +1.2 638,778 - Sama_0519 0.15 +0.2 638,778 - Sama_0519 0.15 -0.6 638,793 + Sama_0519 0.18 -2.5 638,793 + Sama_0519 0.18 -1.2 638,793 + Sama_0519 0.18 +1.1 638,793 + Sama_0519 0.18 -1.5 638,794 - Sama_0519 0.18 +0.4 638,794 - Sama_0519 0.18 +0.3 638,873 - Sama_0519 0.30 -0.5 638,970 + Sama_0519 0.44 +0.1 638,970 + Sama_0519 0.44 +1.1 638,971 - Sama_0519 0.44 +1.1 638,971 - Sama_0519 0.44 +0.8 638,971 - Sama_0519 0.44 -2.0 639,020 + Sama_0519 0.52 +0.4 639,020 + Sama_0519 0.52 +0.6 639,020 + Sama_0519 0.52 -1.4 639,020 + Sama_0519 0.52 -1.4 639,020 + Sama_0519 0.52 +0.2 639,020 + Sama_0519 0.52 -1.5 639,020 + Sama_0519 0.52 +1.2 639,021 - Sama_0519 0.52 -2.1 639,021 - Sama_0519 0.52 -2.2 639,093 + Sama_0519 0.63 +1.3 639,093 + Sama_0519 0.63 -0.8 639,093 + Sama_0519 0.63 -1.0 639,093 + Sama_0519 0.63 +2.2 639,093 + Sama_0519 0.63 -0.8 639,093 + Sama_0519 0.63 +1.4 639,094 - Sama_0519 0.63 -0.5 639,094 - Sama_0519 0.63 -0.5 639,094 - Sama_0519 0.63 -0.2 639,094 - Sama_0519 0.63 +0.5 639,094 - Sama_0519 0.63 -1.2 639,094 - Sama_0519 0.63 -0.5 639,094 - Sama_0519 0.63 +1.4 639,094 - Sama_0519 0.63 -0.8 639,094 - Sama_0519 0.63 -1.2 639,094 - Sama_0519 0.63 -3.6 639,094 - Sama_0519 0.63 +0.9 639,094 - Sama_0519 0.63 +2.5 639,176 - Sama_0519 0.75 -2.2 639,271 + Sama_0519 0.89 -2.8 639,271 + Sama_0519 0.89 +1.5 639,271 + Sama_0519 0.89 +3.4 639,271 + Sama_0519 0.89 -0.4 639,271 + Sama_0519 0.89 -1.8 639,272 - Sama_0519 0.90 +0.4 639,272 - Sama_0519 0.90 -2.2 639,339 - +0.4 639,339 - -2.7 639,339 - -3.3 639,410 + +1.0 639,410 + -2.4 639,410 + -0.6 639,410 + -0.9 639,411 - -0.5 639,411 - -0.2 639,411 - +3.6 639,411 - -1.5 639,440 - +2.5 639,460 + +0.2 639,460 + +1.1 639,460 + +3.5 639,460 + +0.6 639,460 + +1.5 639,460 + -4.0 639,461 - +0.9 639,461 - -1.4 639,461 - +0.2 639,461 - -1.4 639,483 + +0.7 639,484 - -0.6 639,484 - -2.1 639,527 + +1.6 639,528 - -1.2 639,528 - +0.1 639,528 - +0.2 639,534 + -1.9 639,534 + -0.4 639,535 - -2.9 639,535 - +0.2 639,535 - +2.6 639,535 - +2.5 639,535 - +1.3 639,535 - +2.4 639,535 - +2.1 639,631 + Sama_0520 0.16 +0.6 639,631 + Sama_0520 0.16 +1.0 639,631 + Sama_0520 0.16 +0.3 639,631 + Sama_0520 0.16 -0.9 639,632 - Sama_0520 0.16 -3.1 639,788 + Sama_0520 0.32 -0.7 639,789 - Sama_0520 0.32 -0.5 639,823 - Sama_0520 0.36 -1.1 639,855 + Sama_0520 0.39 +0.3 639,867 + Sama_0520 0.40 +0.4 639,867 + Sama_0520 0.40 -1.9 639,868 - Sama_0520 0.41 +1.0 639,904 + Sama_0520 0.44 +2.6 639,904 + Sama_0520 0.44 +0.2 639,904 + Sama_0520 0.44 +0.8 639,904 + Sama_0520 0.44 +0.7 639,905 - Sama_0520 0.44 +0.9 639,917 + Sama_0520 0.46 -1.6 639,917 + Sama_0520 0.46 -0.6 639,918 - Sama_0520 0.46 +0.1 639,918 - Sama_0520 0.46 +4.0 639,954 + Sama_0520 0.50 -2.3 639,954 + Sama_0520 0.50 +1.0 639,954 + Sama_0520 0.50 -0.6 639,954 + Sama_0520 0.50 -1.1 639,954 + Sama_0520 0.50 -0.9 639,955 - Sama_0520 0.50 -0.0 639,955 - Sama_0520 0.50 -1.0 639,955 - Sama_0520 0.50 +1.5 639,955 - Sama_0520 0.50 +0.6 639,989 + Sama_0520 0.53 +1.4 640,003 + Sama_0520 0.55 +2.6 640,004 - Sama_0520 0.55 -2.2 640,097 + Sama_0520 0.65 -1.3 640,214 + Sama_0520 0.77 +0.1 640,215 - Sama_0520 0.77 -2.5 640,215 - Sama_0520 0.77 -1.4 640,215 - Sama_0520 0.77 -2.0 640,215 - Sama_0520 0.77 +0.6 640,308 + Sama_0520 0.87 +0.8 640,309 - Sama_0520 0.87 -0.2 640,309 - Sama_0520 0.87 -1.8 640,311 + Sama_0520 0.87 -1.6 640,311 + Sama_0520 0.87 +0.4
Or see this region's nucleotide sequence