Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0515 and Sama_0516 are separated by 35 nucleotides Sama_0516 and Sama_0517 are separated by 126 nucleotides Sama_0517 and Sama_0518 are separated by 171 nucleotides Sama_0518 and Sama_0519 are separated by 9 nucleotides
Sama_0515: Sama_0515 - transporter, putative (RefSeq), at 634,698 to 636,065
_0515
Sama_0516: Sama_0516 - single-strand binding protein (RefSeq), at 636,101 to 636,805
_0516
Sama_0517: Sama_0517 - D-beta-hydroxybutyrate dehydrogenase (RefSeq), at 636,932 to 637,732
_0517
Sama_0518: Sama_0518 - 3-oxoacid CoA-transferase (RefSeq), at 637,904 to 638,665
_0518
Sama_0519: Sama_0519 - 3-oxoacid CoA-transferase (RefSeq), at 638,675 to 639,340
_0519
Position (kb) 636
637
638 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 635.962 kb on + strand at 635.963 kb on - strand at 636.000 kb on + strand at 636.028 kb on + strand at 636.810 kb on + strand at 636.819 kb on + strand at 636.819 kb on + strand at 636.819 kb on + strand at 636.820 kb on - strand at 636.820 kb on - strand at 636.820 kb on - strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.821 kb on + strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 636.822 kb on - strand at 637.022 kb on + strand, within Sama_0517 at 637.022 kb on + strand, within Sama_0517 at 637.022 kb on + strand, within Sama_0517 at 637.077 kb on + strand, within Sama_0517 at 637.077 kb on + strand, within Sama_0517 at 637.078 kb on - strand, within Sama_0517 at 637.092 kb on + strand, within Sama_0517 at 637.093 kb on - strand, within Sama_0517 at 637.093 kb on - strand, within Sama_0517 at 637.105 kb on + strand, within Sama_0517 at 637.106 kb on - strand, within Sama_0517 at 637.106 kb on - strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.124 kb on + strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.125 kb on - strand, within Sama_0517 at 637.132 kb on + strand, within Sama_0517 at 637.132 kb on + strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.133 kb on - strand, within Sama_0517 at 637.147 kb on + strand, within Sama_0517 at 637.147 kb on + strand, within Sama_0517 at 637.180 kb on + strand, within Sama_0517 at 637.181 kb on - strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.231 kb on + strand, within Sama_0517 at 637.232 kb on - strand, within Sama_0517 at 637.232 kb on - strand, within Sama_0517 at 637.252 kb on - strand, within Sama_0517 at 637.297 kb on + strand, within Sama_0517 at 637.298 kb on - strand, within Sama_0517 at 637.298 kb on - strand, within Sama_0517 at 637.298 kb on - strand, within Sama_0517 at 637.453 kb on + strand, within Sama_0517 at 637.453 kb on + strand, within Sama_0517 at 637.454 kb on - strand, within Sama_0517 at 637.454 kb on - strand, within Sama_0517 at 637.495 kb on + strand, within Sama_0517 at 637.496 kb on - strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.523 kb on + strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.524 kb on - strand, within Sama_0517 at 637.557 kb on + strand, within Sama_0517 at 637.582 kb on + strand, within Sama_0517 at 637.583 kb on - strand, within Sama_0517 at 637.583 kb on - strand, within Sama_0517 at 637.619 kb on + strand, within Sama_0517 at 637.620 kb on - strand, within Sama_0517 at 637.647 kb on - strand, within Sama_0517 at 637.690 kb on + strand at 637.691 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.710 kb on - strand at 637.733 kb on + strand at 637.733 kb on + strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.734 kb on - strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.762 kb on + strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.763 kb on - strand at 637.867 kb on + strand at 637.867 kb on + strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.868 kb on - strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.879 kb on + strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.880 kb on - strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.882 kb on + strand at 637.883 kb on - strand at 637.883 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.888 kb on - strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.912 kb on + strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.913 kb on - strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.915 kb on + strand at 637.916 kb on - strand at 637.966 kb on + strand at 637.966 kb on + strand at 637.967 kb on - strand at 637.967 kb on - strand at 637.982 kb on + strand, within Sama_0518 at 637.982 kb on + strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 637.983 kb on - strand, within Sama_0518 at 638.051 kb on - strand, within Sama_0518 at 638.135 kb on + strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.136 kb on - strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.189 kb on + strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.190 kb on - strand, within Sama_0518 at 638.203 kb on + strand, within Sama_0518 at 638.299 kb on + strand, within Sama_0518 at 638.301 kb on + strand, within Sama_0518 at 638.313 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.382 kb on - strand, within Sama_0518 at 638.441 kb on - strand, within Sama_0518 at 638.444 kb on + strand, within Sama_0518 at 638.445 kb on - strand, within Sama_0518 at 638.457 kb on + strand, within Sama_0518 at 638.458 kb on - strand, within Sama_0518 at 638.511 kb on + strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.512 kb on - strand, within Sama_0518 at 638.585 kb on + strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.586 kb on - strand, within Sama_0518 at 638.659 kb on + strand at 638.659 kb on + strand at 638.659 kb on + strand at 638.660 kb on - strand at 638.663 kb on + strand at 638.663 kb on + strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.664 kb on - strand at 638.732 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 635,962 + -1.6 635,963 - -1.2 636,000 + -1.9 636,028 + -2.9 636,810 + -0.7 636,819 + +0.7 636,819 + -0.9 636,819 + -0.4 636,820 - +0.1 636,820 - +1.6 636,820 - +0.7 636,821 + +0.8 636,821 + -1.2 636,821 + -1.9 636,821 + -3.1 636,821 + -0.1 636,821 + +0.4 636,821 + -2.7 636,821 + +0.5 636,821 + -2.9 636,821 + +3.0 636,821 + -0.5 636,821 + +1.4 636,821 + -1.9 636,821 + +0.4 636,821 + -0.2 636,821 + +0.2 636,821 + +0.2 636,821 + +0.6 636,822 - -0.2 636,822 - +1.0 636,822 - -0.2 636,822 - +0.8 636,822 - +1.8 636,822 - -0.9 637,022 + Sama_0517 0.11 -1.4 637,022 + Sama_0517 0.11 -0.2 637,022 + Sama_0517 0.11 +3.4 637,077 + Sama_0517 0.18 -0.2 637,077 + Sama_0517 0.18 -1.4 637,078 - Sama_0517 0.18 -1.5 637,092 + Sama_0517 0.20 +0.2 637,093 - Sama_0517 0.20 +1.4 637,093 - Sama_0517 0.20 -0.6 637,105 + Sama_0517 0.22 -0.2 637,106 - Sama_0517 0.22 -2.8 637,106 - Sama_0517 0.22 +1.3 637,124 + Sama_0517 0.24 -2.1 637,124 + Sama_0517 0.24 -0.4 637,124 + Sama_0517 0.24 -1.5 637,124 + Sama_0517 0.24 -0.6 637,125 - Sama_0517 0.24 +0.1 637,125 - Sama_0517 0.24 +1.4 637,125 - Sama_0517 0.24 +0.4 637,125 - Sama_0517 0.24 -1.2 637,132 + Sama_0517 0.25 +1.9 637,132 + Sama_0517 0.25 +0.1 637,133 - Sama_0517 0.25 +2.8 637,133 - Sama_0517 0.25 -1.2 637,133 - Sama_0517 0.25 +1.3 637,133 - Sama_0517 0.25 -0.0 637,133 - Sama_0517 0.25 -0.2 637,147 + Sama_0517 0.27 -0.2 637,147 + Sama_0517 0.27 -1.2 637,180 + Sama_0517 0.31 -3.9 637,181 - Sama_0517 0.31 +1.3 637,231 + Sama_0517 0.37 +0.1 637,231 + Sama_0517 0.37 -2.5 637,231 + Sama_0517 0.37 -1.1 637,231 + Sama_0517 0.37 +0.6 637,231 + Sama_0517 0.37 -1.6 637,232 - Sama_0517 0.37 -1.6 637,232 - Sama_0517 0.37 +1.6 637,252 - Sama_0517 0.40 -2.1 637,297 + Sama_0517 0.46 +0.2 637,298 - Sama_0517 0.46 -0.6 637,298 - Sama_0517 0.46 +1.1 637,298 - Sama_0517 0.46 -1.2 637,453 + Sama_0517 0.65 -0.0 637,453 + Sama_0517 0.65 +1.5 637,454 - Sama_0517 0.65 -1.4 637,454 - Sama_0517 0.65 +1.3 637,495 + Sama_0517 0.70 -1.2 637,496 - Sama_0517 0.70 -1.6 637,523 + Sama_0517 0.74 +1.4 637,523 + Sama_0517 0.74 +4.0 637,523 + Sama_0517 0.74 -1.6 637,523 + Sama_0517 0.74 +1.0 637,524 - Sama_0517 0.74 -1.8 637,524 - Sama_0517 0.74 -0.7 637,524 - Sama_0517 0.74 +0.9 637,524 - Sama_0517 0.74 +1.3 637,524 - Sama_0517 0.74 -0.2 637,524 - Sama_0517 0.74 -2.2 637,557 + Sama_0517 0.78 -1.9 637,582 + Sama_0517 0.81 +1.8 637,583 - Sama_0517 0.81 -1.6 637,583 - Sama_0517 0.81 +0.6 637,619 + Sama_0517 0.86 +0.2 637,620 - Sama_0517 0.86 +2.2 637,647 - Sama_0517 0.89 +1.4 637,690 + +0.7 637,691 - +1.8 637,710 - -0.6 637,710 - -0.3 637,710 - +1.3 637,710 - +0.4 637,733 + -0.0 637,733 + +2.7 637,734 - +0.9 637,734 - +0.8 637,734 - +0.7 637,762 + -2.9 637,762 + -2.4 637,762 + +4.4 637,762 + +1.2 637,762 + +1.9 637,762 + -0.0 637,762 + +0.4 637,762 + +0.7 637,762 + -2.9 637,762 + -0.3 637,763 - +1.3 637,763 - -1.6 637,763 - -0.6 637,763 - +0.0 637,763 - +0.3 637,763 - -0.9 637,763 - +1.4 637,763 - +2.0 637,763 - +1.2 637,763 - -1.0 637,763 - +1.6 637,763 - +1.9 637,867 + -0.9 637,867 + +0.4 637,868 - -0.2 637,868 - -1.3 637,868 - +0.2 637,868 - +0.6 637,868 - -0.0 637,879 + -1.2 637,879 + -1.2 637,879 + -1.9 637,879 + +0.3 637,879 + +2.3 637,879 + +0.7 637,879 + +0.3 637,879 + -2.3 637,879 + -1.8 637,879 + +1.0 637,880 - -0.9 637,880 - -3.0 637,880 - -0.9 637,880 - +1.4 637,880 - +1.7 637,880 - +0.6 637,880 - -0.2 637,880 - +0.8 637,880 - -2.1 637,880 - +0.4 637,882 + -0.9 637,882 + -1.1 637,882 + -0.2 637,882 + +0.4 637,883 - +0.4 637,883 - +1.5 637,888 - +0.4 637,888 - -0.8 637,888 - +2.6 637,888 - +0.2 637,888 - -0.9 637,888 - -1.4 637,912 + -1.6 637,912 + +2.0 637,912 + -1.1 637,912 + +3.7 637,912 + +1.2 637,913 - +0.2 637,913 - -0.1 637,913 - -0.9 637,915 + -0.7 637,915 + +0.2 637,915 + -1.3 637,915 + +0.5 637,916 - -2.1 637,966 + -0.3 637,966 + +0.4 637,967 - -1.1 637,967 - -0.1 637,982 + Sama_0518 0.10 -0.8 637,982 + Sama_0518 0.10 -0.3 637,983 - Sama_0518 0.10 +0.8 637,983 - Sama_0518 0.10 +2.2 637,983 - Sama_0518 0.10 -1.5 637,983 - Sama_0518 0.10 +1.8 638,051 - Sama_0518 0.19 -0.6 638,135 + Sama_0518 0.30 +0.6 638,136 - Sama_0518 0.30 +0.7 638,136 - Sama_0518 0.30 -2.5 638,189 + Sama_0518 0.37 +2.0 638,189 + Sama_0518 0.37 -0.9 638,189 + Sama_0518 0.37 -2.6 638,189 + Sama_0518 0.37 -0.7 638,189 + Sama_0518 0.37 +0.1 638,190 - Sama_0518 0.38 +1.3 638,190 - Sama_0518 0.38 -1.8 638,190 - Sama_0518 0.38 -0.8 638,190 - Sama_0518 0.38 +0.3 638,190 - Sama_0518 0.38 +1.2 638,190 - Sama_0518 0.38 -1.9 638,203 + Sama_0518 0.39 +0.2 638,299 + Sama_0518 0.52 +1.9 638,301 + Sama_0518 0.52 -1.2 638,313 - Sama_0518 0.54 -1.1 638,382 - Sama_0518 0.63 +1.4 638,382 - Sama_0518 0.63 -3.1 638,441 - Sama_0518 0.70 -0.4 638,444 + Sama_0518 0.71 -1.8 638,445 - Sama_0518 0.71 +1.8 638,457 + Sama_0518 0.73 +0.1 638,458 - Sama_0518 0.73 +0.5 638,511 + Sama_0518 0.80 +0.4 638,512 - Sama_0518 0.80 -0.4 638,512 - Sama_0518 0.80 -0.5 638,512 - Sama_0518 0.80 +1.5 638,512 - Sama_0518 0.80 -0.7 638,512 - Sama_0518 0.80 +2.3 638,585 + Sama_0518 0.89 +0.3 638,586 - Sama_0518 0.90 -2.3 638,586 - Sama_0518 0.90 -2.2 638,586 - Sama_0518 0.90 +0.3 638,659 + +1.6 638,659 + +1.1 638,659 + -0.1 638,660 - -0.2 638,663 + -1.4 638,663 + -0.6 638,664 - +1.2 638,664 - +1.8 638,664 - +1.9 638,732 + +0.8
Or see this region's nucleotide sequence