Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0319 and Sama_0320 are separated by 141 nucleotides Sama_0320 and pyrE are separated by 106 nucleotides pyrE and folE are separated by 101 nucleotides
Sama_0319: Sama_0319 - hypothetical protein (RefSeq), at 386,521 to 387,450
_0319
Sama_0320: Sama_0320 - tRNA nucleotidyltransferase (RefSeq), at 387,592 to 388,305
_0320
Sama_0321: pyrE - orotate phosphoribosyltransferase (RefSeq), at 388,412 to 389,053
pyrE
Sama_0322: folE - GTP cyclohydrolase I (RefSeq), at 389,155 to 389,805
folE
Position (kb) 387
388
389 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 386.652 kb on + strand, within Sama_0319 at 386.652 kb on + strand, within Sama_0319 at 386.653 kb on - strand, within Sama_0319 at 386.784 kb on + strand, within Sama_0319 at 387.039 kb on + strand, within Sama_0319 at 387.039 kb on + strand, within Sama_0319 at 387.058 kb on + strand, within Sama_0319 at 387.058 kb on + strand, within Sama_0319 at 387.058 kb on + strand, within Sama_0319 at 387.058 kb on + strand, within Sama_0319 at 387.058 kb on + strand, within Sama_0319 at 387.059 kb on - strand, within Sama_0319 at 387.059 kb on - strand, within Sama_0319 at 387.060 kb on + strand, within Sama_0319 at 387.060 kb on + strand, within Sama_0319 at 387.060 kb on + strand, within Sama_0319 at 387.060 kb on + strand, within Sama_0319 at 387.061 kb on - strand, within Sama_0319 at 387.061 kb on - strand, within Sama_0319 at 387.061 kb on - strand, within Sama_0319 at 387.061 kb on - strand, within Sama_0319 at 387.075 kb on + strand, within Sama_0319 at 387.076 kb on - strand, within Sama_0319 at 387.076 kb on - strand, within Sama_0319 at 387.183 kb on - strand, within Sama_0319 at 387.200 kb on - strand, within Sama_0319 at 387.226 kb on + strand, within Sama_0319 at 387.226 kb on + strand, within Sama_0319 at 387.226 kb on + strand, within Sama_0319 at 387.226 kb on + strand, within Sama_0319 at 387.226 kb on + strand, within Sama_0319 at 387.227 kb on - strand, within Sama_0319 at 387.281 kb on + strand, within Sama_0319 at 387.281 kb on + strand, within Sama_0319 at 387.281 kb on + strand, within Sama_0319 at 387.282 kb on - strand, within Sama_0319 at 387.282 kb on - strand, within Sama_0319 at 387.282 kb on - strand, within Sama_0319 at 387.282 kb on - strand, within Sama_0319 at 387.282 kb on - strand, within Sama_0319 at 387.294 kb on - strand, within Sama_0319 at 387.345 kb on + strand, within Sama_0319 at 387.345 kb on + strand, within Sama_0319 at 387.345 kb on + strand, within Sama_0319 at 387.346 kb on - strand, within Sama_0319 at 387.346 kb on - strand, within Sama_0319 at 387.346 kb on - strand, within Sama_0319 at 387.384 kb on + strand at 387.408 kb on + strand at 387.409 kb on - strand at 387.424 kb on + strand at 387.424 kb on + strand at 387.424 kb on + strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.425 kb on - strand at 387.426 kb on + strand at 387.426 kb on + strand at 387.426 kb on + strand at 387.427 kb on - strand at 387.476 kb on + strand at 387.489 kb on + strand at 387.490 kb on - strand at 387.536 kb on + strand at 387.537 kb on - strand at 387.537 kb on - strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.542 kb on + strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.543 kb on - strand at 387.544 kb on + strand at 387.545 kb on - strand at 387.545 kb on - strand at 387.545 kb on - strand at 387.545 kb on - strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.547 kb on + strand at 387.548 kb on - strand at 387.574 kb on + strand at 387.574 kb on + strand at 387.574 kb on + strand at 387.575 kb on - strand at 387.645 kb on + strand at 387.652 kb on - strand at 387.731 kb on + strand, within Sama_0320 at 387.731 kb on + strand, within Sama_0320 at 387.744 kb on + strand, within Sama_0320 at 387.779 kb on - strand, within Sama_0320 at 387.798 kb on + strand, within Sama_0320 at 387.799 kb on - strand, within Sama_0320 at 387.811 kb on - strand, within Sama_0320 at 387.902 kb on + strand, within Sama_0320 at 387.903 kb on - strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.926 kb on + strand, within Sama_0320 at 387.927 kb on - strand, within Sama_0320 at 387.927 kb on - strand, within Sama_0320 at 387.945 kb on + strand, within Sama_0320 at 387.978 kb on + strand, within Sama_0320 at 387.979 kb on - strand, within Sama_0320 at 387.979 kb on - strand, within Sama_0320 at 387.979 kb on - strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.120 kb on + strand, within Sama_0320 at 388.281 kb on + strand at 388.348 kb on + strand at 388.368 kb on + strand at 388.369 kb on - strand at 388.399 kb on + strand at 388.400 kb on - strand at 388.400 kb on - strand at 388.421 kb on + strand at 388.435 kb on + strand at 388.456 kb on + strand at 388.457 kb on - strand at 388.457 kb on - strand at 388.462 kb on - strand at 388.480 kb on + strand, within pyrE at 388.509 kb on - strand, within pyrE at 388.509 kb on - strand, within pyrE at 388.509 kb on - strand, within pyrE at 388.531 kb on + strand, within pyrE at 388.531 kb on + strand, within pyrE at 388.532 kb on - strand, within pyrE at 388.532 kb on - strand, within pyrE at 388.535 kb on - strand, within pyrE at 388.569 kb on - strand, within pyrE at 388.569 kb on - strand, within pyrE at 388.625 kb on + strand, within pyrE at 388.625 kb on + strand, within pyrE at 388.626 kb on - strand, within pyrE at 388.626 kb on - strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.627 kb on + strand, within pyrE at 388.628 kb on - strand, within pyrE at 388.628 kb on - strand, within pyrE at 388.628 kb on - strand, within pyrE at 388.695 kb on - strand, within pyrE at 388.702 kb on + strand, within pyrE at 388.708 kb on + strand, within pyrE at 388.708 kb on + strand, within pyrE at 388.709 kb on - strand, within pyrE at 388.709 kb on - strand, within pyrE at 388.738 kb on + strand, within pyrE at 388.738 kb on + strand, within pyrE at 388.765 kb on + strand, within pyrE at 388.765 kb on + strand, within pyrE at 388.766 kb on - strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.771 kb on + strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 388.772 kb on - strand, within pyrE at 389.022 kb on + strand at 389.030 kb on + strand at 389.030 kb on + strand at 389.051 kb on + strand at 389.051 kb on + strand at 389.051 kb on + strand at 389.052 kb on - strand at 389.052 kb on - strand at 389.064 kb on + strand at 389.064 kb on + strand at 389.064 kb on + strand at 389.071 kb on + strand at 389.100 kb on + strand at 389.101 kb on - strand at 389.101 kb on - strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.144 kb on + strand at 389.145 kb on - strand at 389.145 kb on - strand at 389.150 kb on + strand at 389.151 kb on - strand at 389.151 kb on - strand at 389.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 386,652 + Sama_0319 0.14 +1.2 386,652 + Sama_0319 0.14 +2.0 386,653 - Sama_0319 0.14 +1.0 386,784 + Sama_0319 0.28 -0.3 387,039 + Sama_0319 0.56 +2.1 387,039 + Sama_0319 0.56 -3.0 387,058 + Sama_0319 0.58 +1.5 387,058 + Sama_0319 0.58 +1.5 387,058 + Sama_0319 0.58 +1.7 387,058 + Sama_0319 0.58 +2.2 387,058 + Sama_0319 0.58 +2.7 387,059 - Sama_0319 0.58 +3.8 387,059 - Sama_0319 0.58 +0.2 387,060 + Sama_0319 0.58 -1.3 387,060 + Sama_0319 0.58 -0.8 387,060 + Sama_0319 0.58 +0.4 387,060 + Sama_0319 0.58 +3.5 387,061 - Sama_0319 0.58 +3.7 387,061 - Sama_0319 0.58 +4.1 387,061 - Sama_0319 0.58 +0.8 387,061 - Sama_0319 0.58 +2.0 387,075 + Sama_0319 0.60 +0.2 387,076 - Sama_0319 0.60 -0.5 387,076 - Sama_0319 0.60 +2.5 387,183 - Sama_0319 0.71 +2.5 387,200 - Sama_0319 0.73 +0.8 387,226 + Sama_0319 0.76 +0.7 387,226 + Sama_0319 0.76 +2.8 387,226 + Sama_0319 0.76 -0.5 387,226 + Sama_0319 0.76 -0.0 387,226 + Sama_0319 0.76 -0.8 387,227 - Sama_0319 0.76 +1.5 387,281 + Sama_0319 0.82 -1.8 387,281 + Sama_0319 0.82 -0.6 387,281 + Sama_0319 0.82 +0.8 387,282 - Sama_0319 0.82 -0.5 387,282 - Sama_0319 0.82 +1.5 387,282 - Sama_0319 0.82 +3.9 387,282 - Sama_0319 0.82 +1.4 387,282 - Sama_0319 0.82 +2.1 387,294 - Sama_0319 0.83 +1.7 387,345 + Sama_0319 0.89 -2.0 387,345 + Sama_0319 0.89 +1.1 387,345 + Sama_0319 0.89 -2.2 387,346 - Sama_0319 0.89 +1.0 387,346 - Sama_0319 0.89 +1.5 387,346 - Sama_0319 0.89 +3.4 387,384 + +1.2 387,408 + +1.5 387,409 - -2.2 387,424 + +1.0 387,424 + -1.3 387,424 + -1.0 387,425 - -2.9 387,425 - +3.1 387,425 - -0.0 387,425 - -0.9 387,425 - -1.6 387,425 - -1.9 387,425 - +1.5 387,425 - -1.0 387,426 + +1.4 387,426 + +1.4 387,426 + +2.8 387,427 - -0.9 387,476 + -0.3 387,489 + +1.0 387,490 - +0.8 387,536 + -2.8 387,537 - -1.6 387,537 - +0.5 387,542 + -0.2 387,542 + -1.1 387,542 + -1.3 387,542 + -1.3 387,542 + -1.8 387,542 + +0.0 387,542 + +1.6 387,542 + -0.5 387,543 - -1.8 387,543 - -1.0 387,543 - +0.6 387,543 - -0.0 387,543 - -1.9 387,543 - -1.2 387,543 - +1.1 387,544 + -1.9 387,545 - -1.0 387,545 - -3.2 387,545 - -2.5 387,545 - -3.0 387,547 + -0.8 387,547 + -1.5 387,547 + +1.5 387,547 + -0.0 387,547 + -0.2 387,547 + +0.5 387,548 - +0.0 387,574 + -0.5 387,574 + -1.5 387,574 + +0.5 387,575 - -0.5 387,645 + -1.4 387,652 - +0.5 387,731 + Sama_0320 0.19 -1.6 387,731 + Sama_0320 0.19 -0.5 387,744 + Sama_0320 0.21 -0.8 387,779 - Sama_0320 0.26 -1.3 387,798 + Sama_0320 0.29 -0.5 387,799 - Sama_0320 0.29 -0.5 387,811 - Sama_0320 0.31 -0.5 387,902 + Sama_0320 0.43 -1.9 387,903 - Sama_0320 0.44 -0.0 387,926 + Sama_0320 0.47 +0.3 387,926 + Sama_0320 0.47 -0.3 387,926 + Sama_0320 0.47 +1.5 387,926 + Sama_0320 0.47 -1.5 387,927 - Sama_0320 0.47 -0.1 387,927 - Sama_0320 0.47 -0.0 387,945 + Sama_0320 0.49 -1.8 387,978 + Sama_0320 0.54 -0.5 387,979 - Sama_0320 0.54 -2.0 387,979 - Sama_0320 0.54 -1.0 387,979 - Sama_0320 0.54 -3.0 388,120 + Sama_0320 0.74 -0.0 388,120 + Sama_0320 0.74 -1.0 388,120 + Sama_0320 0.74 -0.3 388,120 + Sama_0320 0.74 -0.6 388,120 + Sama_0320 0.74 -1.3 388,281 + +0.5 388,348 + -3.7 388,368 + -2.2 388,369 - -0.0 388,399 + +1.9 388,400 - +0.9 388,400 - -1.5 388,421 + +1.7 388,435 + -2.3 388,456 + -1.9 388,457 - -1.0 388,457 - +2.1 388,462 - +1.5 388,480 + pyrE Sama_0321 0.11 +1.3 388,509 - pyrE Sama_0321 0.15 -0.9 388,509 - pyrE Sama_0321 0.15 +1.1 388,509 - pyrE Sama_0321 0.15 +1.5 388,531 + pyrE Sama_0321 0.19 +2.3 388,531 + pyrE Sama_0321 0.19 +1.4 388,532 - pyrE Sama_0321 0.19 +1.1 388,532 - pyrE Sama_0321 0.19 +0.3 388,535 - pyrE Sama_0321 0.19 +0.9 388,569 - pyrE Sama_0321 0.24 -1.5 388,569 - pyrE Sama_0321 0.24 -0.1 388,625 + pyrE Sama_0321 0.33 +1.8 388,625 + pyrE Sama_0321 0.33 +2.4 388,626 - pyrE Sama_0321 0.33 +0.9 388,626 - pyrE Sama_0321 0.33 +1.2 388,627 + pyrE Sama_0321 0.33 +2.9 388,627 + pyrE Sama_0321 0.33 -1.8 388,627 + pyrE Sama_0321 0.33 -0.3 388,627 + pyrE Sama_0321 0.33 -0.2 388,628 - pyrE Sama_0321 0.34 -1.5 388,628 - pyrE Sama_0321 0.34 +2.8 388,628 - pyrE Sama_0321 0.34 -2.4 388,695 - pyrE Sama_0321 0.44 +2.8 388,702 + pyrE Sama_0321 0.45 +2.5 388,708 + pyrE Sama_0321 0.46 -2.8 388,708 + pyrE Sama_0321 0.46 -0.0 388,709 - pyrE Sama_0321 0.46 +3.8 388,709 - pyrE Sama_0321 0.46 -2.5 388,738 + pyrE Sama_0321 0.51 -1.6 388,738 + pyrE Sama_0321 0.51 +0.4 388,765 + pyrE Sama_0321 0.55 -1.3 388,765 + pyrE Sama_0321 0.55 +1.0 388,766 - pyrE Sama_0321 0.55 -1.8 388,771 + pyrE Sama_0321 0.56 -2.5 388,771 + pyrE Sama_0321 0.56 -0.5 388,771 + pyrE Sama_0321 0.56 +4.6 388,771 + pyrE Sama_0321 0.56 -1.8 388,772 - pyrE Sama_0321 0.56 -2.0 388,772 - pyrE Sama_0321 0.56 -1.3 388,772 - pyrE Sama_0321 0.56 +1.2 388,772 - pyrE Sama_0321 0.56 -1.0 388,772 - pyrE Sama_0321 0.56 +1.3 388,772 - pyrE Sama_0321 0.56 -1.0 389,022 + +1.9 389,030 + -1.8 389,030 + +0.1 389,051 + +0.0 389,051 + -1.3 389,051 + +0.4 389,052 - +1.5 389,052 - -0.5 389,064 + -1.4 389,064 + -0.5 389,064 + -0.5 389,071 + +0.6 389,100 + -1.5 389,101 - +0.4 389,101 - -2.8 389,144 + -0.8 389,144 + -0.0 389,144 + +0.2 389,144 + +1.0 389,144 + +1.1 389,144 + -2.0 389,145 - -1.0 389,145 - -2.1 389,150 + -1.0 389,151 - -0.3 389,151 - -0.7 389,151 - -0.5
Or see this region's nucleotide sequence