Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.3730.1 and Xcc-8004.3731.1 are separated by 8 nucleotides Xcc-8004.3731.1 and Xcc-8004.3732.1 overlap by 1 nucleotides Xcc-8004.3732.1 and Xcc-8004.3733.1 are separated by 39 nucleotides
Xcc-8004.3730.1: Xcc-8004.3730.1 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 3,605,566 to 3,606,639
Xcc-8004.3730.1
Xcc-8004.3731.1: Xcc-8004.3731.1 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 3,606,648 to 3,608,570
Xcc-8004.3731.1
Xcc-8004.3732.1: Xcc-8004.3732.1 - HpaP protein; Type III secretion protein (YscP), at 3,608,570 to 3,609,205
Xcc-8004.3732.1
Xcc-8004.3733.1: Xcc-8004.3733.1 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 3,609,245 to 3,610,252
Xcc-8004.3733.1
Position (kb)
3606
3607
3608
3609 Strain fitness (log2 ratio)
-2
-1
0
1 at 3605.707 kb on + strand, within Xcc-8004.3730.1 at 3605.708 kb on - strand, within Xcc-8004.3730.1 at 3606.638 kb on - strand at 3606.673 kb on - strand at 3606.756 kb on - strand at 3606.810 kb on + strand at 3606.811 kb on - strand at 3606.811 kb on - strand at 3606.811 kb on - strand at 3607.282 kb on + strand, within Xcc-8004.3731.1 at 3607.596 kb on - strand, within Xcc-8004.3731.1 at 3608.214 kb on + strand, within Xcc-8004.3731.1 at 3608.464 kb on - strand at 3608.508 kb on + strand at 3609.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 3,605,707 + Xcc-8004.3730.1 0.13 +0.3 3,605,708 - Xcc-8004.3730.1 0.13 +1.6 3,606,638 - -1.8 3,606,673 - -1.8 3,606,756 - -1.1 3,606,810 + -1.1 3,606,811 - -0.3 3,606,811 - +0.6 3,606,811 - -1.6 3,607,282 + Xcc-8004.3731.1 0.33 -0.9 3,607,596 - Xcc-8004.3731.1 0.49 -0.4 3,608,214 + Xcc-8004.3731.1 0.81 +0.3 3,608,464 - -0.4 3,608,508 + -0.8 3,609,148 + -0.1
Or see this region's nucleotide sequence