Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS03185

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS03180 and PFLU_RS03185 overlap by 4 nucleotidesPFLU_RS03185 and PFLU_RS03190 are separated by 19 nucleotides PFLU_RS03180: PFLU_RS03180 - hypothetical protein, at 735,153 to 736,346 _RS03180 PFLU_RS03185: PFLU_RS03185 - type II and III secretion system protein family protein, at 736,343 to 737,560 _RS03185 PFLU_RS03190: PFLU_RS03190 - Flp pilus assembly protein CpaB, at 737,580 to 738,521 _RS03190 Position (kb) 736 737 738Strain fitness (log2 ratio) -1 0 1 2 3at 735.406 kb on + strand, within PFLU_RS03180at 735.406 kb on + strand, within PFLU_RS03180at 735.406 kb on + strand, within PFLU_RS03180at 735.407 kb on - strand, within PFLU_RS03180at 735.407 kb on - strand, within PFLU_RS03180at 735.407 kb on - strand, within PFLU_RS03180at 735.407 kb on - strand, within PFLU_RS03180at 735.450 kb on - strand, within PFLU_RS03180at 735.677 kb on - strand, within PFLU_RS03180at 735.734 kb on + strand, within PFLU_RS03180at 735.735 kb on - strand, within PFLU_RS03180at 735.806 kb on + strand, within PFLU_RS03180at 735.807 kb on - strand, within PFLU_RS03180at 736.021 kb on + strand, within PFLU_RS03180at 736.021 kb on + strand, within PFLU_RS03180at 736.416 kb on + strandat 736.416 kb on + strandat 736.417 kb on - strandat 736.657 kb on + strand, within PFLU_RS03185at 736.657 kb on + strand, within PFLU_RS03185at 736.657 kb on + strand, within PFLU_RS03185at 736.658 kb on - strand, within PFLU_RS03185at 736.722 kb on + strand, within PFLU_RS03185at 736.722 kb on + strand, within PFLU_RS03185at 736.722 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.819 kb on - strand, within PFLU_RS03185at 736.819 kb on - strand, within PFLU_RS03185at 736.936 kb on - strand, within PFLU_RS03185at 736.936 kb on - strand, within PFLU_RS03185at 737.093 kb on + strand, within PFLU_RS03185at 737.094 kb on - strand, within PFLU_RS03185at 737.279 kb on + strand, within PFLU_RS03185at 737.280 kb on - strand, within PFLU_RS03185at 737.280 kb on - strand, within PFLU_RS03185at 737.359 kb on + strand, within PFLU_RS03185at 737.547 kb on + strandat 737.704 kb on + strand, within PFLU_RS03190at 737.704 kb on + strand, within PFLU_RS03190at 737.704 kb on + strand, within PFLU_RS03190at 737.761 kb on + strand, within PFLU_RS03190at 737.793 kb on + strand, within PFLU_RS03190at 737.794 kb on - strand, within PFLU_RS03190at 737.873 kb on + strand, within PFLU_RS03190at 737.873 kb on + strand, within PFLU_RS03190at 737.874 kb on - strand, within PFLU_RS03190at 737.874 kb on - strand, within PFLU_RS03190at 738.029 kb on + strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.447 kb on + strandat 738.447 kb on + strandat 738.559 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
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735,406 + PFLU_RS03180 0.21 +2.0
735,406 + PFLU_RS03180 0.21 +1.0
735,406 + PFLU_RS03180 0.21 +1.1
735,407 - PFLU_RS03180 0.21 +1.2
735,407 - PFLU_RS03180 0.21 +2.1
735,407 - PFLU_RS03180 0.21 +1.1
735,407 - PFLU_RS03180 0.21 +0.0
735,450 - PFLU_RS03180 0.25 +1.9
735,677 - PFLU_RS03180 0.44 +0.7
735,734 + PFLU_RS03180 0.49 +2.0
735,735 - PFLU_RS03180 0.49 +1.8
735,806 + PFLU_RS03180 0.55 +0.8
735,807 - PFLU_RS03180 0.55 +0.9
736,021 + PFLU_RS03180 0.73 +1.9
736,021 + PFLU_RS03180 0.73 +1.8
736,416 + +0.2
736,416 + +0.8
736,417 - +0.4
736,657 + PFLU_RS03185 0.26 +1.3
736,657 + PFLU_RS03185 0.26 +1.0
736,657 + PFLU_RS03185 0.26 +1.2
736,658 - PFLU_RS03185 0.26 +0.1
736,722 + PFLU_RS03185 0.31 +0.4
736,722 + PFLU_RS03185 0.31 +0.8
736,722 + PFLU_RS03185 0.31 +0.8
736,818 + PFLU_RS03185 0.39 +0.9
736,818 + PFLU_RS03185 0.39 +1.6
736,818 + PFLU_RS03185 0.39 +0.5
736,818 + PFLU_RS03185 0.39 +1.4
736,818 + PFLU_RS03185 0.39 +1.2
736,818 + PFLU_RS03185 0.39 +1.5
736,819 - PFLU_RS03185 0.39 +1.4
736,819 - PFLU_RS03185 0.39 +1.0
736,936 - PFLU_RS03185 0.49 +1.1
736,936 - PFLU_RS03185 0.49 +1.4
737,093 + PFLU_RS03185 0.62 +1.4
737,094 - PFLU_RS03185 0.62 +0.1
737,279 + PFLU_RS03185 0.77 +1.2
737,280 - PFLU_RS03185 0.77 +1.1
737,280 - PFLU_RS03185 0.77 +1.6
737,359 + PFLU_RS03185 0.83 +1.9
737,547 + +1.5
737,704 + PFLU_RS03190 0.13 +2.4
737,704 + PFLU_RS03190 0.13 +0.7
737,704 + PFLU_RS03190 0.13 +0.3
737,761 + PFLU_RS03190 0.19 +1.0
737,793 + PFLU_RS03190 0.23 +1.9
737,794 - PFLU_RS03190 0.23 +1.9
737,873 + PFLU_RS03190 0.31 +1.2
737,873 + PFLU_RS03190 0.31 +1.4
737,874 - PFLU_RS03190 0.31 +0.7
737,874 - PFLU_RS03190 0.31 +1.9
738,029 + PFLU_RS03190 0.48 +0.9
738,030 - PFLU_RS03190 0.48 +0.8
738,030 - PFLU_RS03190 0.48 +1.9
738,030 - PFLU_RS03190 0.48 +2.3
738,030 - PFLU_RS03190 0.48 +1.0
738,067 + PFLU_RS03190 0.52 +0.2
738,067 + PFLU_RS03190 0.52 +2.7
738,067 + PFLU_RS03190 0.52 +1.1
738,067 + PFLU_RS03190 0.52 +2.8
738,447 + +1.2
738,447 + +1.5
738,559 + +1.3

Or see this region's nucleotide sequence