Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03135 and PFLU_RS03140 overlap by 4 nucleotides PFLU_RS03140 and PFLU_RS03145 are separated by 72 nucleotides PFLU_RS03145 and PFLU_RS03150 are separated by 126 nucleotides
PFLU_RS03135: PFLU_RS03135 - PAS domain-containing sensor histidine kinase, at 726,452 to 729,190
_RS03135
PFLU_RS03140: PFLU_RS03140 - pilus assembly protein, at 729,187 to 729,630
_RS03140
PFLU_RS03145: PFLU_RS03145 - peptidase A24, at 729,703 to 730,152
_RS03145
PFLU_RS03150: PFLU_RS03150 - response regulator transcription factor, at 730,279 to 731,067
_RS03150
Position (kb) 729
730 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 728.213 kb on + strand, within PFLU_RS03135 at 728.213 kb on + strand, within PFLU_RS03135 at 728.213 kb on + strand, within PFLU_RS03135 at 728.213 kb on + strand, within PFLU_RS03135 at 728.214 kb on - strand, within PFLU_RS03135 at 728.214 kb on - strand, within PFLU_RS03135 at 728.214 kb on - strand, within PFLU_RS03135 at 728.214 kb on - strand, within PFLU_RS03135 at 728.214 kb on - strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.409 kb on + strand, within PFLU_RS03135 at 728.410 kb on - strand, within PFLU_RS03135 at 728.410 kb on - strand, within PFLU_RS03135 at 728.410 kb on - strand, within PFLU_RS03135 at 728.410 kb on - strand, within PFLU_RS03135 at 728.455 kb on - strand, within PFLU_RS03135 at 728.488 kb on + strand, within PFLU_RS03135 at 728.489 kb on - strand, within PFLU_RS03135 at 728.616 kb on + strand, within PFLU_RS03135 at 728.616 kb on + strand, within PFLU_RS03135 at 728.617 kb on - strand, within PFLU_RS03135 at 728.617 kb on - strand, within PFLU_RS03135 at 728.617 kb on - strand, within PFLU_RS03135 at 728.617 kb on - strand, within PFLU_RS03135 at 728.625 kb on + strand, within PFLU_RS03135 at 728.625 kb on + strand, within PFLU_RS03135 at 728.625 kb on + strand, within PFLU_RS03135 at 728.625 kb on + strand, within PFLU_RS03135 at 728.625 kb on + strand, within PFLU_RS03135 at 728.625 kb on + strand, within PFLU_RS03135 at 728.626 kb on - strand, within PFLU_RS03135 at 728.626 kb on - strand, within PFLU_RS03135 at 729.064 kb on + strand at 729.064 kb on + strand at 729.176 kb on - strand at 729.278 kb on + strand, within PFLU_RS03140 at 729.278 kb on + strand, within PFLU_RS03140 at 729.278 kb on + strand, within PFLU_RS03140 at 729.278 kb on + strand, within PFLU_RS03140 at 729.279 kb on - strand, within PFLU_RS03140 at 729.279 kb on - strand, within PFLU_RS03140 at 729.297 kb on - strand, within PFLU_RS03140 at 729.329 kb on + strand, within PFLU_RS03140 at 729.329 kb on + strand, within PFLU_RS03140 at 729.329 kb on + strand, within PFLU_RS03140 at 729.329 kb on + strand, within PFLU_RS03140 at 729.329 kb on + strand, within PFLU_RS03140 at 729.330 kb on - strand, within PFLU_RS03140 at 729.330 kb on - strand, within PFLU_RS03140 at 729.330 kb on - strand, within PFLU_RS03140 at 729.330 kb on - strand, within PFLU_RS03140 at 729.427 kb on - strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.431 kb on + strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.432 kb on - strand, within PFLU_RS03140 at 729.528 kb on + strand, within PFLU_RS03140 at 729.528 kb on + strand, within PFLU_RS03140 at 729.528 kb on + strand, within PFLU_RS03140 at 729.528 kb on + strand, within PFLU_RS03140 at 729.529 kb on - strand, within PFLU_RS03140 at 729.529 kb on - strand, within PFLU_RS03140 at 729.529 kb on - strand, within PFLU_RS03140 at 729.529 kb on - strand, within PFLU_RS03140 at 729.529 kb on - strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.530 kb on + strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.531 kb on - strand, within PFLU_RS03140 at 729.545 kb on + strand, within PFLU_RS03140 at 729.545 kb on + strand, within PFLU_RS03140 at 729.546 kb on - strand, within PFLU_RS03140 at 729.546 kb on - strand, within PFLU_RS03140 at 729.546 kb on - strand, within PFLU_RS03140 at 729.546 kb on - strand, within PFLU_RS03140 at 729.587 kb on - strand at 729.641 kb on - strand at 729.641 kb on - strand at 729.652 kb on + strand at 729.652 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.655 kb on + strand at 729.656 kb on - strand at 729.656 kb on - strand at 729.656 kb on - strand at 729.656 kb on - strand at 729.656 kb on - strand at 729.670 kb on + strand at 729.670 kb on + strand at 729.670 kb on + strand at 729.670 kb on + strand at 729.670 kb on + strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.671 kb on - strand at 729.701 kb on - strand at 729.722 kb on + strand at 729.722 kb on + strand at 729.723 kb on - strand at 729.723 kb on - strand at 729.817 kb on + strand, within PFLU_RS03145 at 729.817 kb on + strand, within PFLU_RS03145 at 729.817 kb on + strand, within PFLU_RS03145 at 729.818 kb on - strand, within PFLU_RS03145 at 729.818 kb on - strand, within PFLU_RS03145 at 729.818 kb on - strand, within PFLU_RS03145 at 729.818 kb on - strand, within PFLU_RS03145 at 729.818 kb on - strand, within PFLU_RS03145 at 729.837 kb on + strand, within PFLU_RS03145 at 729.837 kb on + strand, within PFLU_RS03145 at 729.837 kb on + strand, within PFLU_RS03145 at 729.837 kb on + strand, within PFLU_RS03145 at 729.838 kb on - strand, within PFLU_RS03145 at 729.838 kb on - strand, within PFLU_RS03145 at 729.838 kb on - strand, within PFLU_RS03145 at 730.036 kb on - strand, within PFLU_RS03145 at 730.112 kb on + strand at 730.113 kb on - strand at 730.113 kb on - strand at 730.132 kb on + strand at 730.132 kb on + strand at 730.133 kb on - strand at 730.133 kb on - strand at 730.150 kb on + strand at 730.168 kb on - strand at 730.168 kb on - strand at 730.168 kb on - strand at 730.177 kb on + strand at 730.177 kb on + strand at 730.177 kb on + strand at 730.177 kb on + strand at 730.177 kb on + strand at 730.177 kb on + strand at 730.177 kb on + strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.178 kb on - strand at 730.192 kb on + strand at 730.192 kb on + strand at 730.192 kb on + strand at 730.192 kb on + strand at 730.193 kb on - strand at 730.193 kb on - strand at 730.193 kb on - strand at 730.193 kb on - strand at 730.193 kb on - strand at 730.193 kb on - strand at 730.193 kb on - strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.198 kb on + strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.199 kb on - strand at 730.204 kb on + strand at 730.205 kb on - strand at 730.219 kb on + strand at 730.220 kb on - strand at 730.273 kb on + strand at 730.274 kb on - strand at 730.289 kb on + strand at 730.289 kb on + strand at 730.289 kb on + strand at 730.290 kb on - strand at 730.290 kb on - strand at 730.290 kb on - strand at 730.290 kb on - strand at 730.290 kb on - strand at 730.290 kb on - strand at 730.373 kb on - strand, within PFLU_RS03150 at 730.603 kb on + strand, within PFLU_RS03150 at 730.603 kb on + strand, within PFLU_RS03150 at 730.604 kb on - strand, within PFLU_RS03150 at 730.604 kb on - strand, within PFLU_RS03150 at 730.604 kb on - strand, within PFLU_RS03150 at 730.604 kb on - strand, within PFLU_RS03150 at 730.604 kb on - strand, within PFLU_RS03150 at 730.606 kb on + strand, within PFLU_RS03150 at 730.606 kb on + strand, within PFLU_RS03150 at 730.606 kb on + strand, within PFLU_RS03150 at 730.607 kb on - strand, within PFLU_RS03150 at 730.607 kb on - strand, within PFLU_RS03150 at 730.607 kb on - strand, within PFLU_RS03150
Per-strain Table
Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs remove 728,213 + PFLU_RS03135 0.64 +1.1 728,213 + PFLU_RS03135 0.64 +0.6 728,213 + PFLU_RS03135 0.64 +1.2 728,213 + PFLU_RS03135 0.64 +1.9 728,214 - PFLU_RS03135 0.64 +1.8 728,214 - PFLU_RS03135 0.64 +1.7 728,214 - PFLU_RS03135 0.64 +0.5 728,214 - PFLU_RS03135 0.64 +1.1 728,214 - PFLU_RS03135 0.64 +1.3 728,409 + PFLU_RS03135 0.71 +0.2 728,409 + PFLU_RS03135 0.71 +1.4 728,409 + PFLU_RS03135 0.71 +2.9 728,409 + PFLU_RS03135 0.71 +1.1 728,409 + PFLU_RS03135 0.71 +1.6 728,409 + PFLU_RS03135 0.71 +1.0 728,409 + PFLU_RS03135 0.71 +2.3 728,410 - PFLU_RS03135 0.71 +0.5 728,410 - PFLU_RS03135 0.71 +0.9 728,410 - PFLU_RS03135 0.71 +1.1 728,410 - PFLU_RS03135 0.71 +1.9 728,455 - PFLU_RS03135 0.73 +1.1 728,488 + PFLU_RS03135 0.74 +1.4 728,489 - PFLU_RS03135 0.74 +1.6 728,616 + PFLU_RS03135 0.79 +1.9 728,616 + PFLU_RS03135 0.79 +0.8 728,617 - PFLU_RS03135 0.79 +1.8 728,617 - PFLU_RS03135 0.79 +2.0 728,617 - PFLU_RS03135 0.79 +1.1 728,617 - PFLU_RS03135 0.79 +1.0 728,625 + PFLU_RS03135 0.79 +0.9 728,625 + PFLU_RS03135 0.79 +1.3 728,625 + PFLU_RS03135 0.79 +2.1 728,625 + PFLU_RS03135 0.79 +0.9 728,625 + PFLU_RS03135 0.79 +2.2 728,625 + PFLU_RS03135 0.79 +2.3 728,626 - PFLU_RS03135 0.79 +3.0 728,626 - PFLU_RS03135 0.79 +2.1 729,064 + +2.7 729,064 + +1.5 729,176 - +1.3 729,278 + PFLU_RS03140 0.20 +1.1 729,278 + PFLU_RS03140 0.20 +0.9 729,278 + PFLU_RS03140 0.20 +1.3 729,278 + PFLU_RS03140 0.20 +1.9 729,279 - PFLU_RS03140 0.21 +0.2 729,279 - PFLU_RS03140 0.21 +1.7 729,297 - PFLU_RS03140 0.25 -1.7 729,329 + PFLU_RS03140 0.32 +0.5 729,329 + PFLU_RS03140 0.32 +1.2 729,329 + PFLU_RS03140 0.32 +1.1 729,329 + PFLU_RS03140 0.32 +1.0 729,329 + PFLU_RS03140 0.32 -0.1 729,330 - PFLU_RS03140 0.32 +1.1 729,330 - PFLU_RS03140 0.32 +1.2 729,330 - PFLU_RS03140 0.32 +0.9 729,330 - PFLU_RS03140 0.32 +2.2 729,427 - PFLU_RS03140 0.54 +0.6 729,431 + PFLU_RS03140 0.55 +0.9 729,431 + PFLU_RS03140 0.55 +1.8 729,431 + PFLU_RS03140 0.55 -0.2 729,431 + PFLU_RS03140 0.55 +1.5 729,431 + PFLU_RS03140 0.55 +1.8 729,431 + PFLU_RS03140 0.55 +1.2 729,431 + PFLU_RS03140 0.55 +1.9 729,431 + PFLU_RS03140 0.55 +3.1 729,431 + PFLU_RS03140 0.55 +2.8 729,431 + PFLU_RS03140 0.55 +2.0 729,432 - PFLU_RS03140 0.55 +1.3 729,432 - PFLU_RS03140 0.55 +1.3 729,432 - PFLU_RS03140 0.55 +1.2 729,432 - PFLU_RS03140 0.55 +0.4 729,432 - PFLU_RS03140 0.55 +1.1 729,432 - PFLU_RS03140 0.55 +0.7 729,432 - PFLU_RS03140 0.55 +0.4 729,432 - PFLU_RS03140 0.55 +1.5 729,432 - PFLU_RS03140 0.55 +1.5 729,432 - PFLU_RS03140 0.55 -0.8 729,432 - PFLU_RS03140 0.55 +1.2 729,432 - PFLU_RS03140 0.55 +1.6 729,528 + PFLU_RS03140 0.77 +1.7 729,528 + PFLU_RS03140 0.77 +1.9 729,528 + PFLU_RS03140 0.77 +1.8 729,528 + PFLU_RS03140 0.77 +2.6 729,529 - PFLU_RS03140 0.77 +2.1 729,529 - PFLU_RS03140 0.77 +1.2 729,529 - PFLU_RS03140 0.77 +1.6 729,529 - PFLU_RS03140 0.77 +2.0 729,529 - PFLU_RS03140 0.77 +1.5 729,530 + PFLU_RS03140 0.77 +1.6 729,530 + PFLU_RS03140 0.77 +2.2 729,530 + PFLU_RS03140 0.77 +1.8 729,530 + PFLU_RS03140 0.77 +1.2 729,530 + PFLU_RS03140 0.77 +0.4 729,530 + PFLU_RS03140 0.77 +2.5 729,530 + PFLU_RS03140 0.77 +1.3 729,530 + PFLU_RS03140 0.77 +0.6 729,530 + PFLU_RS03140 0.77 +1.6 729,530 + PFLU_RS03140 0.77 +0.8 729,530 + PFLU_RS03140 0.77 +1.5 729,531 - PFLU_RS03140 0.77 +1.8 729,531 - PFLU_RS03140 0.77 +0.5 729,531 - PFLU_RS03140 0.77 +2.0 729,531 - PFLU_RS03140 0.77 +1.2 729,531 - PFLU_RS03140 0.77 +1.8 729,531 - PFLU_RS03140 0.77 +0.9 729,531 - PFLU_RS03140 0.77 +1.3 729,531 - PFLU_RS03140 0.77 +0.8 729,531 - PFLU_RS03140 0.77 +1.8 729,531 - PFLU_RS03140 0.77 +1.3 729,531 - PFLU_RS03140 0.77 +0.3 729,531 - PFLU_RS03140 0.77 +0.9 729,545 + PFLU_RS03140 0.81 +0.8 729,545 + PFLU_RS03140 0.81 +1.1 729,546 - PFLU_RS03140 0.81 +1.6 729,546 - PFLU_RS03140 0.81 +2.3 729,546 - PFLU_RS03140 0.81 +1.9 729,546 - PFLU_RS03140 0.81 +1.4 729,587 - +0.6 729,641 - +0.9 729,641 - +0.5 729,652 + +0.7 729,652 + -1.4 729,655 + +1.2 729,655 + +0.5 729,655 + +0.9 729,655 + +1.2 729,655 + +0.2 729,655 + +1.5 729,655 + -0.6 729,655 + +1.6 729,655 + +0.3 729,655 + -0.9 729,655 + +0.9 729,656 - +0.1 729,656 - -0.5 729,656 - +1.9 729,656 - -1.5 729,656 - -0.9 729,670 + +0.6 729,670 + -1.5 729,670 + -0.2 729,670 + -2.3 729,670 + -0.1 729,671 - -0.3 729,671 - +1.8 729,671 - +0.6 729,671 - +0.2 729,671 - +0.2 729,671 - -0.4 729,671 - -0.1 729,671 - +0.6 729,701 - +1.0 729,722 + +0.9 729,722 + +1.7 729,723 - -0.2 729,723 - +0.4 729,817 + PFLU_RS03145 0.25 +3.8 729,817 + PFLU_RS03145 0.25 +0.6 729,817 + PFLU_RS03145 0.25 +1.3 729,818 - PFLU_RS03145 0.26 +2.9 729,818 - PFLU_RS03145 0.26 +0.7 729,818 - PFLU_RS03145 0.26 +0.7 729,818 - PFLU_RS03145 0.26 +2.1 729,818 - PFLU_RS03145 0.26 +1.1 729,837 + PFLU_RS03145 0.30 +1.3 729,837 + PFLU_RS03145 0.30 +0.6 729,837 + PFLU_RS03145 0.30 +0.9 729,837 + PFLU_RS03145 0.30 +1.8 729,838 - PFLU_RS03145 0.30 +1.5 729,838 - PFLU_RS03145 0.30 +1.0 729,838 - PFLU_RS03145 0.30 -0.1 730,036 - PFLU_RS03145 0.74 +1.1 730,112 + +1.7 730,113 - +0.5 730,113 - +1.7 730,132 + +1.3 730,132 + +0.6 730,133 - +2.4 730,133 - +1.6 730,150 + -0.8 730,168 - -1.8 730,168 - +1.0 730,168 - +1.2 730,177 + +0.8 730,177 + +0.8 730,177 + -0.9 730,177 + -2.3 730,177 + -0.6 730,177 + -1.2 730,177 + -0.2 730,178 - +0.5 730,178 - +3.0 730,178 - +0.6 730,178 - +1.7 730,178 - +1.8 730,178 - +2.4 730,178 - +2.5 730,178 - +1.3 730,178 - +1.9 730,178 - -1.7 730,192 + -0.1 730,192 + -1.9 730,192 + +0.9 730,192 + +0.6 730,193 - +1.9 730,193 - -0.1 730,193 - +2.5 730,193 - +1.4 730,193 - +0.7 730,193 - +2.0 730,193 - +1.6 730,198 + -0.2 730,198 + -1.0 730,198 + +0.8 730,198 + +0.8 730,198 + -2.6 730,198 + -0.8 730,198 + -0.2 730,198 + -0.1 730,198 + -0.8 730,198 + -2.2 730,198 + +0.0 730,198 + -1.1 730,198 + -0.7 730,199 - +3.2 730,199 - +2.7 730,199 - +1.6 730,199 - +1.4 730,199 - +2.2 730,199 - +0.7 730,199 - +0.5 730,199 - +1.7 730,199 - +1.0 730,199 - +2.2 730,204 + -1.0 730,205 - +1.3 730,219 + +1.5 730,220 - +1.2 730,273 + +0.5 730,274 - -1.2 730,289 + +2.0 730,289 + +0.9 730,289 + +2.1 730,290 - +1.3 730,290 - +1.3 730,290 - +1.4 730,290 - +0.2 730,290 - +1.6 730,290 - +0.1 730,373 - PFLU_RS03150 0.12 +1.1 730,603 + PFLU_RS03150 0.41 +1.9 730,603 + PFLU_RS03150 0.41 +1.5 730,604 - PFLU_RS03150 0.41 +0.1 730,604 - PFLU_RS03150 0.41 +2.9 730,604 - PFLU_RS03150 0.41 +1.3 730,604 - PFLU_RS03150 0.41 +1.4 730,604 - PFLU_RS03150 0.41 +0.8 730,606 + PFLU_RS03150 0.41 +2.0 730,606 + PFLU_RS03150 0.41 +0.6 730,606 + PFLU_RS03150 0.41 +2.5 730,607 - PFLU_RS03150 0.42 +1.7 730,607 - PFLU_RS03150 0.42 +0.4 730,607 - PFLU_RS03150 0.42 +0.8
Or see this region's nucleotide sequence