Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01320 and PGA1_262p01330 overlap by 14 nucleotides PGA1_262p01330 and PGA1_262p01340 are separated by 81 nucleotides PGA1_262p01340 and PGA1_262p01350 are separated by 80 nucleotides
PGA1_262p01320: PGA1_262p01320 - Uncharacterized protein conserved in bacteria, at 143,235 to 143,936
_262p01320
PGA1_262p01330: PGA1_262p01330 - transcriptional regulator, MarR family, at 143,923 to 144,480
_262p01330
PGA1_262p01340: PGA1_262p01340 - hypothetical protein, at 144,562 to 145,311
_262p01340
PGA1_262p01350: PGA1_262p01350 - putative vanillate O-demethylase oxidoreductase, at 145,392 to 146,336
_262p01350
Position (kb) 144
145
146 Strain fitness (log2 ratio) -1
0
1 at 143.739 kb on + strand, within PGA1_262p01320 at 143.935 kb on + strand at 144.118 kb on + strand, within PGA1_262p01330 at 144.604 kb on - strand at 144.650 kb on + strand, within PGA1_262p01340 at 144.650 kb on + strand, within PGA1_262p01340 at 144.658 kb on - strand, within PGA1_262p01340 at 144.827 kb on + strand, within PGA1_262p01340 at 144.830 kb on - strand, within PGA1_262p01340 at 144.830 kb on - strand, within PGA1_262p01340 at 144.865 kb on - strand, within PGA1_262p01340 at 144.922 kb on + strand, within PGA1_262p01340 at 145.005 kb on - strand, within PGA1_262p01340 at 145.176 kb on - strand, within PGA1_262p01340 at 145.177 kb on - strand, within PGA1_262p01340 at 145.189 kb on + strand, within PGA1_262p01340 at 145.213 kb on - strand, within PGA1_262p01340 at 145.619 kb on + strand, within PGA1_262p01350
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 143,739 + PGA1_262p01320 0.72 +0.6 143,935 + +0.1 144,118 + PGA1_262p01330 0.35 -0.5 144,604 - -0.8 144,650 + PGA1_262p01340 0.12 +0.5 144,650 + PGA1_262p01340 0.12 -0.1 144,658 - PGA1_262p01340 0.13 +0.8 144,827 + PGA1_262p01340 0.35 -0.8 144,830 - PGA1_262p01340 0.36 +1.5 144,830 - PGA1_262p01340 0.36 -0.1 144,865 - PGA1_262p01340 0.40 +1.0 144,922 + PGA1_262p01340 0.48 -0.4 145,005 - PGA1_262p01340 0.59 -0.4 145,176 - PGA1_262p01340 0.82 +1.1 145,177 - PGA1_262p01340 0.82 -0.3 145,189 + PGA1_262p01340 0.84 +1.3 145,213 - PGA1_262p01340 0.87 +0.4 145,619 + PGA1_262p01350 0.24 -0.6
Or see this region's nucleotide sequence