Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c31150 and PGA1_c31160 overlap by 1 nucleotides PGA1_c31160 and PGA1_c31170 overlap by 4 nucleotides PGA1_c31170 and PGA1_c31180 are separated by 3 nucleotides PGA1_c31180 and PGA1_c31190 are separated by 162 nucleotides
PGA1_c31150: PGA1_c31150 - Acetyltransferase (GNAT) family., at 3,253,373 to 3,253,963
_c31150
PGA1_c31160: PGA1_c31160 - conserved hypothetical protein, steroid delta-isomerase-related, at 3,253,963 to 3,254,358
_c31160
PGA1_c31170: PGA1_c31170 - phosphotyrosine protein phosphatase-like protein, at 3,254,355 to 3,254,813
_c31170
PGA1_c31180: PGA1_c31180 - Uncharacterized protein conserved in bacteria, at 3,254,817 to 3,255,296
_c31180
PGA1_c31190: PGA1_c31190 - histidinol dehydrogenase HisD, at 3,255,459 to 3,256,760
_c31190
Position (kb)
3254
3255
3256 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3253.828 kb on - strand, within PGA1_c31150 at 3253.833 kb on - strand, within PGA1_c31150 at 3253.840 kb on + strand, within PGA1_c31150 at 3253.844 kb on + strand, within PGA1_c31150 at 3253.864 kb on + strand, within PGA1_c31150 at 3253.872 kb on - strand, within PGA1_c31150 at 3253.874 kb on + strand, within PGA1_c31150 at 3253.911 kb on + strand at 3253.927 kb on + strand at 3253.956 kb on + strand at 3253.956 kb on + strand at 3253.967 kb on + strand at 3253.967 kb on + strand at 3253.967 kb on + strand at 3253.967 kb on + strand at 3253.972 kb on + strand at 3253.972 kb on + strand at 3253.975 kb on - strand at 3253.975 kb on - strand at 3253.975 kb on - strand at 3253.980 kb on - strand at 3253.980 kb on - strand at 3253.980 kb on - strand at 3253.995 kb on + strand at 3253.995 kb on + strand at 3253.995 kb on + strand at 3253.995 kb on + strand at 3253.995 kb on + strand at 3254.003 kb on - strand, within PGA1_c31160 at 3254.003 kb on - strand, within PGA1_c31160 at 3254.003 kb on - strand, within PGA1_c31160 at 3254.003 kb on - strand, within PGA1_c31160 at 3254.061 kb on + strand, within PGA1_c31160 at 3254.061 kb on + strand, within PGA1_c31160 at 3254.061 kb on + strand, within PGA1_c31160 at 3254.061 kb on + strand, within PGA1_c31160 at 3254.069 kb on - strand, within PGA1_c31160 at 3254.069 kb on - strand, within PGA1_c31160 at 3254.069 kb on - strand, within PGA1_c31160 at 3254.069 kb on - strand, within PGA1_c31160 at 3254.089 kb on - strand, within PGA1_c31160 at 3254.170 kb on + strand, within PGA1_c31160 at 3254.184 kb on - strand, within PGA1_c31160 at 3254.198 kb on - strand, within PGA1_c31160 at 3254.203 kb on + strand, within PGA1_c31160 at 3254.211 kb on - strand, within PGA1_c31160 at 3254.301 kb on - strand, within PGA1_c31160 at 3254.328 kb on + strand at 3254.372 kb on + strand at 3254.377 kb on + strand at 3254.377 kb on + strand at 3254.385 kb on - strand at 3254.449 kb on + strand, within PGA1_c31170 at 3254.449 kb on + strand, within PGA1_c31170 at 3254.459 kb on + strand, within PGA1_c31170 at 3254.467 kb on - strand, within PGA1_c31170 at 3254.581 kb on + strand, within PGA1_c31170 at 3254.581 kb on + strand, within PGA1_c31170 at 3254.630 kb on + strand, within PGA1_c31170 at 3254.751 kb on - strand, within PGA1_c31170 at 3254.782 kb on - strand at 3254.956 kb on + strand, within PGA1_c31180 at 3254.956 kb on + strand, within PGA1_c31180 at 3254.956 kb on + strand, within PGA1_c31180 at 3254.980 kb on + strand, within PGA1_c31180 at 3255.017 kb on - strand, within PGA1_c31180 at 3255.040 kb on - strand, within PGA1_c31180 at 3255.056 kb on + strand, within PGA1_c31180 at 3255.063 kb on + strand, within PGA1_c31180 at 3255.104 kb on - strand, within PGA1_c31180 at 3255.184 kb on + strand, within PGA1_c31180 at 3255.298 kb on + strand at 3255.298 kb on + strand at 3255.298 kb on + strand at 3255.306 kb on - strand at 3255.306 kb on - strand at 3255.306 kb on - strand at 3255.331 kb on - strand at 3255.341 kb on - strand at 3255.344 kb on + strand at 3255.367 kb on + strand at 3255.367 kb on + strand at 3255.440 kb on + strand at 3255.451 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 3,253,828 - PGA1_c31150 0.77 -1.9 3,253,833 - PGA1_c31150 0.78 +0.3 3,253,840 + PGA1_c31150 0.79 -0.0 3,253,844 + PGA1_c31150 0.80 +0.0 3,253,864 + PGA1_c31150 0.83 +0.8 3,253,872 - PGA1_c31150 0.84 +0.0 3,253,874 + PGA1_c31150 0.85 -0.5 3,253,911 + -1.0 3,253,927 + -0.2 3,253,956 + +0.1 3,253,956 + -0.4 3,253,967 + +1.8 3,253,967 + -0.2 3,253,967 + -0.2 3,253,967 + -0.9 3,253,972 + -1.3 3,253,972 + +0.7 3,253,975 - +0.2 3,253,975 - +1.0 3,253,975 - +0.2 3,253,980 - -0.3 3,253,980 - +0.1 3,253,980 - -1.1 3,253,995 + -0.5 3,253,995 + -0.3 3,253,995 + -0.3 3,253,995 + +1.4 3,253,995 + -0.2 3,254,003 - PGA1_c31160 0.10 +0.1 3,254,003 - PGA1_c31160 0.10 -1.4 3,254,003 - PGA1_c31160 0.10 +0.2 3,254,003 - PGA1_c31160 0.10 +0.1 3,254,061 + PGA1_c31160 0.25 +0.8 3,254,061 + PGA1_c31160 0.25 -0.2 3,254,061 + PGA1_c31160 0.25 +0.2 3,254,061 + PGA1_c31160 0.25 -0.3 3,254,069 - PGA1_c31160 0.27 -1.5 3,254,069 - PGA1_c31160 0.27 +0.1 3,254,069 - PGA1_c31160 0.27 +0.2 3,254,069 - PGA1_c31160 0.27 -0.8 3,254,089 - PGA1_c31160 0.32 +0.0 3,254,170 + PGA1_c31160 0.52 +0.8 3,254,184 - PGA1_c31160 0.56 +0.3 3,254,198 - PGA1_c31160 0.59 -0.2 3,254,203 + PGA1_c31160 0.61 -1.2 3,254,211 - PGA1_c31160 0.63 -1.5 3,254,301 - PGA1_c31160 0.85 +0.3 3,254,328 + -0.2 3,254,372 + -0.5 3,254,377 + +1.0 3,254,377 + -0.2 3,254,385 - +0.7 3,254,449 + PGA1_c31170 0.20 +0.8 3,254,449 + PGA1_c31170 0.20 -0.3 3,254,459 + PGA1_c31170 0.23 -0.3 3,254,467 - PGA1_c31170 0.24 +0.4 3,254,581 + PGA1_c31170 0.49 -0.8 3,254,581 + PGA1_c31170 0.49 +0.7 3,254,630 + PGA1_c31170 0.60 +0.3 3,254,751 - PGA1_c31170 0.86 +0.4 3,254,782 - +1.4 3,254,956 + PGA1_c31180 0.29 +0.4 3,254,956 + PGA1_c31180 0.29 +1.2 3,254,956 + PGA1_c31180 0.29 -0.2 3,254,980 + PGA1_c31180 0.34 -0.4 3,255,017 - PGA1_c31180 0.42 +0.9 3,255,040 - PGA1_c31180 0.46 +0.5 3,255,056 + PGA1_c31180 0.50 +0.9 3,255,063 + PGA1_c31180 0.51 +0.7 3,255,104 - PGA1_c31180 0.60 +0.4 3,255,184 + PGA1_c31180 0.76 -0.3 3,255,298 + +1.5 3,255,298 + -0.1 3,255,298 + +0.2 3,255,306 - +1.0 3,255,306 - +0.9 3,255,306 - -0.6 3,255,331 - +0.2 3,255,341 - +1.2 3,255,344 + -0.2 3,255,367 + +0.2 3,255,367 + +0.0 3,255,440 + +1.1 3,255,451 - +0.6
Or see this region's nucleotide sequence