Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12130 and PGA1_c12140 are separated by 28 nucleotides PGA1_c12140 and PGA1_c12150 overlap by 4 nucleotides PGA1_c12150 and PGA1_c12160 overlap by 4 nucleotides PGA1_c12160 and PGA1_c12170 are separated by 152 nucleotides
PGA1_c12130: PGA1_c12130 - DNA-damage-inducible protein F, at 1,259,514 to 1,260,845
_c12130
PGA1_c12140: PGA1_c12140 - putative arsenate reductase, at 1,260,874 to 1,261,215
_c12140
PGA1_c12150: PGA1_c12150 - dihydroorotate dehydrogenase PyrD, at 1,261,212 to 1,262,270
_c12150
PGA1_c12160: PGA1_c12160 - Uncharacterized protein conserved in bacteria, at 1,262,267 to 1,262,605
_c12160
PGA1_c12170: PGA1_c12170 - putative 5'-nucleotidase, at 1,262,758 to 1,264,335
_c12170
Position (kb)
1261
1262
1263 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1260.218 kb on + strand, within PGA1_c12130 at 1260.218 kb on + strand, within PGA1_c12130 at 1260.244 kb on - strand, within PGA1_c12130 at 1260.249 kb on + strand, within PGA1_c12130 at 1260.378 kb on - strand, within PGA1_c12130 at 1260.431 kb on - strand, within PGA1_c12130 at 1260.457 kb on + strand, within PGA1_c12130 at 1260.457 kb on + strand, within PGA1_c12130 at 1260.498 kb on + strand, within PGA1_c12130 at 1260.501 kb on - strand, within PGA1_c12130 at 1260.595 kb on + strand, within PGA1_c12130 at 1260.609 kb on + strand, within PGA1_c12130 at 1260.668 kb on - strand, within PGA1_c12130 at 1260.793 kb on + strand at 1260.793 kb on + strand at 1260.793 kb on + strand at 1260.799 kb on + strand at 1260.799 kb on + strand at 1260.801 kb on - strand at 1260.801 kb on - strand at 1260.807 kb on - strand at 1260.884 kb on + strand at 1260.940 kb on + strand, within PGA1_c12140 at 1260.955 kb on + strand, within PGA1_c12140 at 1261.014 kb on - strand, within PGA1_c12140 at 1261.058 kb on + strand, within PGA1_c12140 at 1261.084 kb on - strand, within PGA1_c12140 at 1261.114 kb on + strand, within PGA1_c12140 at 1261.145 kb on - strand, within PGA1_c12140 at 1261.145 kb on - strand, within PGA1_c12140 at 1261.151 kb on + strand, within PGA1_c12140 at 1261.151 kb on + strand, within PGA1_c12140 at 1261.159 kb on - strand, within PGA1_c12140 at 1261.159 kb on - strand, within PGA1_c12140 at 1261.161 kb on + strand, within PGA1_c12140 at 1261.161 kb on + strand, within PGA1_c12140 at 1261.193 kb on + strand at 1261.200 kb on + strand at 1261.329 kb on + strand, within PGA1_c12150 at 1261.329 kb on + strand, within PGA1_c12150 at 1261.329 kb on + strand, within PGA1_c12150 at 1261.337 kb on - strand, within PGA1_c12150 at 1261.337 kb on - strand, within PGA1_c12150 at 1261.339 kb on - strand, within PGA1_c12150 at 1261.386 kb on - strand, within PGA1_c12150 at 1261.391 kb on - strand, within PGA1_c12150 at 1261.391 kb on - strand, within PGA1_c12150 at 1261.489 kb on + strand, within PGA1_c12150 at 1261.525 kb on + strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.535 kb on - strand, within PGA1_c12150 at 1261.546 kb on + strand, within PGA1_c12150 at 1261.658 kb on - strand, within PGA1_c12150 at 1261.681 kb on + strand, within PGA1_c12150 at 1261.693 kb on + strand, within PGA1_c12150 at 1261.693 kb on + strand, within PGA1_c12150 at 1261.701 kb on - strand, within PGA1_c12150 at 1261.715 kb on - strand, within PGA1_c12150 at 1261.717 kb on + strand, within PGA1_c12150 at 1261.717 kb on + strand, within PGA1_c12150 at 1261.725 kb on - strand, within PGA1_c12150 at 1261.747 kb on - strand, within PGA1_c12150 at 1261.839 kb on + strand, within PGA1_c12150 at 1261.839 kb on + strand, within PGA1_c12150 at 1261.989 kb on - strand, within PGA1_c12150 at 1261.989 kb on - strand, within PGA1_c12150 at 1262.024 kb on + strand, within PGA1_c12150 at 1262.085 kb on + strand, within PGA1_c12150 at 1262.114 kb on + strand, within PGA1_c12150 at 1262.144 kb on + strand, within PGA1_c12150 at 1262.240 kb on - strand at 1262.255 kb on - strand at 1262.264 kb on + strand at 1262.264 kb on + strand at 1262.322 kb on + strand, within PGA1_c12160 at 1262.324 kb on - strand, within PGA1_c12160 at 1262.330 kb on - strand, within PGA1_c12160 at 1262.401 kb on + strand, within PGA1_c12160 at 1262.404 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.409 kb on - strand, within PGA1_c12160 at 1262.437 kb on + strand, within PGA1_c12160 at 1262.437 kb on + strand, within PGA1_c12160 at 1262.612 kb on + strand at 1262.612 kb on + strand at 1262.612 kb on + strand at 1262.620 kb on - strand at 1262.620 kb on - strand at 1262.620 kb on - strand at 1262.625 kb on + strand at 1262.668 kb on + strand at 1262.721 kb on + strand at 1262.745 kb on + strand at 1262.797 kb on - strand at 1262.859 kb on + strand at 1262.902 kb on - strand at 1262.922 kb on - strand, within PGA1_c12170 at 1262.927 kb on - strand, within PGA1_c12170 at 1262.946 kb on + strand, within PGA1_c12170 at 1262.993 kb on - strand, within PGA1_c12170 at 1263.014 kb on + strand, within PGA1_c12170 at 1263.030 kb on + strand, within PGA1_c12170 at 1263.030 kb on + strand, within PGA1_c12170 at 1263.032 kb on + strand, within PGA1_c12170 at 1263.038 kb on - strand, within PGA1_c12170 at 1263.200 kb on + strand, within PGA1_c12170
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 1,260,218 + PGA1_c12130 0.53 +0.2 1,260,218 + PGA1_c12130 0.53 -0.9 1,260,244 - PGA1_c12130 0.55 -0.9 1,260,249 + PGA1_c12130 0.55 +0.4 1,260,378 - PGA1_c12130 0.65 -2.0 1,260,431 - PGA1_c12130 0.69 -0.7 1,260,457 + PGA1_c12130 0.71 -1.1 1,260,457 + PGA1_c12130 0.71 +0.2 1,260,498 + PGA1_c12130 0.74 -0.9 1,260,501 - PGA1_c12130 0.74 +0.6 1,260,595 + PGA1_c12130 0.81 +1.0 1,260,609 + PGA1_c12130 0.82 +0.4 1,260,668 - PGA1_c12130 0.87 +0.0 1,260,793 + -0.7 1,260,793 + +0.8 1,260,793 + -0.4 1,260,799 + +0.9 1,260,799 + -2.1 1,260,801 - -0.9 1,260,801 - +0.2 1,260,807 - -0.6 1,260,884 + -1.1 1,260,940 + PGA1_c12140 0.19 -1.9 1,260,955 + PGA1_c12140 0.24 -0.3 1,261,014 - PGA1_c12140 0.41 +0.5 1,261,058 + PGA1_c12140 0.54 +0.7 1,261,084 - PGA1_c12140 0.61 -0.5 1,261,114 + PGA1_c12140 0.70 -1.7 1,261,145 - PGA1_c12140 0.79 +0.5 1,261,145 - PGA1_c12140 0.79 +0.3 1,261,151 + PGA1_c12140 0.81 -0.7 1,261,151 + PGA1_c12140 0.81 -1.2 1,261,159 - PGA1_c12140 0.83 -0.9 1,261,159 - PGA1_c12140 0.83 +0.5 1,261,161 + PGA1_c12140 0.84 -1.1 1,261,161 + PGA1_c12140 0.84 +0.4 1,261,193 + -0.1 1,261,200 + +0.3 1,261,329 + PGA1_c12150 0.11 +0.1 1,261,329 + PGA1_c12150 0.11 -0.1 1,261,329 + PGA1_c12150 0.11 +1.0 1,261,337 - PGA1_c12150 0.12 +0.2 1,261,337 - PGA1_c12150 0.12 +0.8 1,261,339 - PGA1_c12150 0.12 +1.3 1,261,386 - PGA1_c12150 0.16 +0.3 1,261,391 - PGA1_c12150 0.17 +0.3 1,261,391 - PGA1_c12150 0.17 +0.4 1,261,489 + PGA1_c12150 0.26 +0.5 1,261,525 + PGA1_c12150 0.30 +0.1 1,261,535 - PGA1_c12150 0.31 +0.9 1,261,535 - PGA1_c12150 0.31 +0.8 1,261,535 - PGA1_c12150 0.31 +1.1 1,261,535 - PGA1_c12150 0.31 -0.9 1,261,546 + PGA1_c12150 0.32 +0.7 1,261,658 - PGA1_c12150 0.42 +1.0 1,261,681 + PGA1_c12150 0.44 +0.7 1,261,693 + PGA1_c12150 0.45 +0.1 1,261,693 + PGA1_c12150 0.45 -0.1 1,261,701 - PGA1_c12150 0.46 -0.2 1,261,715 - PGA1_c12150 0.47 +0.7 1,261,717 + PGA1_c12150 0.48 +1.2 1,261,717 + PGA1_c12150 0.48 -0.5 1,261,725 - PGA1_c12150 0.48 -0.2 1,261,747 - PGA1_c12150 0.51 +1.6 1,261,839 + PGA1_c12150 0.59 +0.2 1,261,839 + PGA1_c12150 0.59 +0.3 1,261,989 - PGA1_c12150 0.73 +0.9 1,261,989 - PGA1_c12150 0.73 -0.3 1,262,024 + PGA1_c12150 0.77 +0.2 1,262,085 + PGA1_c12150 0.82 -0.2 1,262,114 + PGA1_c12150 0.85 -0.3 1,262,144 + PGA1_c12150 0.88 +0.4 1,262,240 - -0.1 1,262,255 - +2.0 1,262,264 + -1.2 1,262,264 + -0.2 1,262,322 + PGA1_c12160 0.16 +0.4 1,262,324 - PGA1_c12160 0.17 -0.5 1,262,330 - PGA1_c12160 0.19 +0.3 1,262,401 + PGA1_c12160 0.40 -1.7 1,262,404 - PGA1_c12160 0.40 -0.1 1,262,409 - PGA1_c12160 0.42 -0.1 1,262,409 - PGA1_c12160 0.42 -1.2 1,262,409 - PGA1_c12160 0.42 -0.0 1,262,409 - PGA1_c12160 0.42 -2.5 1,262,409 - PGA1_c12160 0.42 -0.2 1,262,437 + PGA1_c12160 0.50 +0.6 1,262,437 + PGA1_c12160 0.50 +1.4 1,262,612 + -1.8 1,262,612 + +1.5 1,262,612 + +0.3 1,262,620 - -0.9 1,262,620 - +0.7 1,262,620 - +0.3 1,262,625 + -0.6 1,262,668 + -0.1 1,262,721 + +1.1 1,262,745 + +0.2 1,262,797 - -0.3 1,262,859 + -0.1 1,262,902 - +0.2 1,262,922 - PGA1_c12170 0.10 +0.7 1,262,927 - PGA1_c12170 0.11 -0.3 1,262,946 + PGA1_c12170 0.12 +1.3 1,262,993 - PGA1_c12170 0.15 +0.1 1,263,014 + PGA1_c12170 0.16 +0.5 1,263,030 + PGA1_c12170 0.17 +0.5 1,263,030 + PGA1_c12170 0.17 +0.8 1,263,032 + PGA1_c12170 0.17 +0.4 1,263,038 - PGA1_c12170 0.18 +0.7 1,263,200 + PGA1_c12170 0.28 -0.3
Or see this region's nucleotide sequence