Experiment: m.b. D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01010 and PGA1_c01020 are separated by 33 nucleotides PGA1_c01020 and PGA1_c01030 are separated by 99 nucleotides PGA1_c01030 and PGA1_c01040 are separated by 59 nucleotides PGA1_c01040 and PGA1_c01050 are separated by 63 nucleotides
PGA1_c01010: PGA1_c01010 - guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase SpoT, at 94,762 to 96,894
_c01010
PGA1_c01020: PGA1_c01020 - Uncharacterized protein conserved in bacteria, at 96,928 to 97,581
_c01020
PGA1_c01030: PGA1_c01030 - pyridoxine 5'-phosphate synthase PdxJ, at 97,681 to 98,442
_c01030
PGA1_c01040: PGA1_c01040 - holo-[acyl-carrier-protein] synthase AcpS, at 98,502 to 98,933
_c01040
PGA1_c01050: PGA1_c01050 - putative signal peptidase I, at 98,997 to 99,833
_c01050
Position (kb) 97
98
99 Strain fitness (log2 ratio) -2
-1
0
1 at 96.681 kb on - strand at 96.681 kb on - strand at 96.681 kb on - strand at 96.684 kb on + strand at 96.684 kb on + strand at 96.692 kb on - strand at 96.727 kb on - strand at 96.858 kb on - strand at 96.858 kb on - strand at 96.858 kb on - strand at 97.487 kb on + strand, within PGA1_c01020 at 97.559 kb on + strand at 97.575 kb on + strand at 97.621 kb on + strand at 97.621 kb on + strand at 97.629 kb on - strand at 97.631 kb on + strand at 97.642 kb on + strand at 98.481 kb on - strand at 98.488 kb on + strand at 98.491 kb on - strand at 98.496 kb on - strand at 98.496 kb on - strand at 98.496 kb on - strand at 98.498 kb on + strand at 98.505 kb on + strand at 98.513 kb on + strand at 98.554 kb on + strand, within PGA1_c01040 at 98.599 kb on + strand, within PGA1_c01040 at 98.607 kb on - strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.609 kb on + strand, within PGA1_c01040 at 98.616 kb on + strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.617 kb on - strand, within PGA1_c01040 at 98.654 kb on + strand, within PGA1_c01040 at 98.685 kb on - strand, within PGA1_c01040 at 98.697 kb on + strand, within PGA1_c01040 at 98.697 kb on + strand, within PGA1_c01040 at 98.800 kb on + strand, within PGA1_c01040 at 98.855 kb on + strand, within PGA1_c01040 at 98.855 kb on + strand, within PGA1_c01040 at 98.855 kb on + strand, within PGA1_c01040 at 98.863 kb on - strand, within PGA1_c01040 at 98.867 kb on + strand, within PGA1_c01040 at 98.875 kb on - strand, within PGA1_c01040
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.0625 mg/ml remove 96,681 - -2.6 96,681 - -0.6 96,681 - -1.1 96,684 + -0.5 96,684 + -0.6 96,692 - -1.1 96,727 - -0.5 96,858 - -1.4 96,858 - +1.3 96,858 - -2.3 97,487 + PGA1_c01020 0.85 -0.6 97,559 + +0.9 97,575 + -0.7 97,621 + -2.3 97,621 + +0.4 97,629 - -2.2 97,631 + +0.3 97,642 + -0.2 98,481 - -0.3 98,488 + -0.4 98,491 - +0.6 98,496 - -1.2 98,496 - +0.5 98,496 - -0.2 98,498 + +0.4 98,505 + +1.0 98,513 + -0.4 98,554 + PGA1_c01040 0.12 +0.5 98,599 + PGA1_c01040 0.22 +0.6 98,607 - PGA1_c01040 0.24 -0.9 98,609 + PGA1_c01040 0.25 +0.7 98,609 + PGA1_c01040 0.25 -0.2 98,609 + PGA1_c01040 0.25 -0.7 98,609 + PGA1_c01040 0.25 -0.3 98,609 + PGA1_c01040 0.25 +0.3 98,609 + PGA1_c01040 0.25 -0.3 98,609 + PGA1_c01040 0.25 -2.5 98,609 + PGA1_c01040 0.25 +0.5 98,609 + PGA1_c01040 0.25 +0.5 98,609 + PGA1_c01040 0.25 -0.2 98,609 + PGA1_c01040 0.25 -2.2 98,609 + PGA1_c01040 0.25 -0.6 98,609 + PGA1_c01040 0.25 -0.6 98,616 + PGA1_c01040 0.26 +0.3 98,617 - PGA1_c01040 0.27 -0.4 98,617 - PGA1_c01040 0.27 -0.0 98,617 - PGA1_c01040 0.27 +0.0 98,617 - PGA1_c01040 0.27 -0.4 98,617 - PGA1_c01040 0.27 +1.6 98,617 - PGA1_c01040 0.27 -0.8 98,617 - PGA1_c01040 0.27 +0.5 98,617 - PGA1_c01040 0.27 +0.5 98,617 - PGA1_c01040 0.27 +0.3 98,617 - PGA1_c01040 0.27 -1.4 98,617 - PGA1_c01040 0.27 -0.3 98,617 - PGA1_c01040 0.27 -0.6 98,617 - PGA1_c01040 0.27 -0.2 98,617 - PGA1_c01040 0.27 -0.4 98,617 - PGA1_c01040 0.27 -1.2 98,617 - PGA1_c01040 0.27 -0.1 98,617 - PGA1_c01040 0.27 -0.2 98,617 - PGA1_c01040 0.27 -0.1 98,654 + PGA1_c01040 0.35 -2.1 98,685 - PGA1_c01040 0.42 -1.3 98,697 + PGA1_c01040 0.45 +0.0 98,697 + PGA1_c01040 0.45 -0.3 98,800 + PGA1_c01040 0.69 +0.8 98,855 + PGA1_c01040 0.82 +0.1 98,855 + PGA1_c01040 0.82 -0.3 98,855 + PGA1_c01040 0.82 -0.8 98,863 - PGA1_c01040 0.84 +1.3 98,867 + PGA1_c01040 0.84 -0.5 98,875 - PGA1_c01040 0.86 -0.7
Or see this region's nucleotide sequence