Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3159 and DvMF_3160 are separated by 339 nucleotides DvMF_3160 and DvMF_3161 are separated by 4 nucleotides DvMF_3161 and DvMF_3162 are separated by 138 nucleotides
DvMF_3159: DvMF_3159 - protein of unknown function DUF610 YibQ (RefSeq), at 3,984,270 to 3,985,901
_3159
DvMF_3160: DvMF_3160 - Nucleoside-diphosphate kinase (RefSeq), at 3,986,241 to 3,986,660
_3160
DvMF_3161: DvMF_3161 - pyrroline-5-carboxylate reductase (EC 1.5.1.2) (from data) , at 3,986,665 to 3,987,474
_3161
DvMF_3162: DvMF_3162 - Smr protein/MutS2 (RefSeq), at 3,987,613 to 3,988,965
_3162
Position (kb)
3986
3987
3988 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3985.831 kb on + strand at 3985.969 kb on + strand at 3985.971 kb on + strand at 3986.149 kb on + strand at 3986.170 kb on - strand at 3986.175 kb on - strand at 3986.934 kb on + strand, within DvMF_3161 at 3987.042 kb on + strand, within DvMF_3161 at 3987.050 kb on - strand, within DvMF_3161 at 3987.191 kb on + strand, within DvMF_3161 at 3987.276 kb on + strand, within DvMF_3161 at 3987.739 kb on - strand at 3987.786 kb on - strand, within DvMF_3162 at 3987.868 kb on - strand, within DvMF_3162 at 3987.873 kb on - strand, within DvMF_3162 at 3987.930 kb on + strand, within DvMF_3162 at 3987.952 kb on + strand, within DvMF_3162 at 3988.155 kb on + strand, within DvMF_3162
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 3,985,831 + -1.5 3,985,969 + -1.3 3,985,971 + -0.4 3,986,149 + +0.5 3,986,170 - +0.0 3,986,175 - -0.9 3,986,934 + DvMF_3161 0.33 -3.9 3,987,042 + DvMF_3161 0.47 -1.9 3,987,050 - DvMF_3161 0.48 -5.4 3,987,191 + DvMF_3161 0.65 -2.8 3,987,276 + DvMF_3161 0.75 -2.7 3,987,739 - -0.8 3,987,786 - DvMF_3162 0.13 -1.5 3,987,868 - DvMF_3162 0.19 +0.4 3,987,873 - DvMF_3162 0.19 +0.1 3,987,930 + DvMF_3162 0.23 -1.6 3,987,952 + DvMF_3162 0.25 -0.6 3,988,155 + DvMF_3162 0.40 -1.9
Or see this region's nucleotide sequence