Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3147 and DvMF_3148 are separated by 148 nucleotides DvMF_3148 and DvMF_3149 are separated by 92 nucleotides DvMF_3149 and DvMF_3150 are separated by 263 nucleotides
DvMF_3147: DvMF_3147 - alpha-glucan phosphorylase (RefSeq), at 3,970,413 to 3,972,983
_3147
DvMF_3148: DvMF_3148 - deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (RefSeq), at 3,973,132 to 3,973,641
_3148
DvMF_3149: DvMF_3149 - acetylornithine aminotransferase (RefSeq), at 3,973,734 to 3,974,942
_3149
DvMF_3150: DvMF_3150 - hypothetical protein (RefSeq), at 3,975,206 to 3,975,556
_3150
Position (kb)
3973
3974
3975 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3972.782 kb on + strand at 3972.782 kb on + strand at 3972.937 kb on + strand at 3973.408 kb on - strand, within DvMF_3148 at 3974.009 kb on + strand, within DvMF_3149 at 3974.026 kb on + strand, within DvMF_3149 at 3974.060 kb on + strand, within DvMF_3149 at 3974.098 kb on - strand, within DvMF_3149 at 3974.171 kb on - strand, within DvMF_3149 at 3974.200 kb on - strand, within DvMF_3149 at 3974.200 kb on - strand, within DvMF_3149 at 3974.205 kb on - strand, within DvMF_3149 at 3974.396 kb on + strand, within DvMF_3149 at 3974.396 kb on + strand, within DvMF_3149 at 3974.429 kb on - strand, within DvMF_3149 at 3974.449 kb on - strand, within DvMF_3149 at 3974.613 kb on + strand, within DvMF_3149 at 3974.751 kb on - strand, within DvMF_3149 at 3974.774 kb on - strand, within DvMF_3149 at 3975.111 kb on + strand at 3975.278 kb on + strand, within DvMF_3150
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 3,972,782 + -2.8 3,972,782 + +0.2 3,972,937 + +0.0 3,973,408 - DvMF_3148 0.54 -0.6 3,974,009 + DvMF_3149 0.23 -2.1 3,974,026 + DvMF_3149 0.24 -4.9 3,974,060 + DvMF_3149 0.27 -2.6 3,974,098 - DvMF_3149 0.30 -4.0 3,974,171 - DvMF_3149 0.36 -3.6 3,974,200 - DvMF_3149 0.39 -4.8 3,974,200 - DvMF_3149 0.39 -6.0 3,974,205 - DvMF_3149 0.39 -2.0 3,974,396 + DvMF_3149 0.55 -1.9 3,974,396 + DvMF_3149 0.55 -2.1 3,974,429 - DvMF_3149 0.57 +0.3 3,974,449 - DvMF_3149 0.59 -4.2 3,974,613 + DvMF_3149 0.73 -2.4 3,974,751 - DvMF_3149 0.84 -2.1 3,974,774 - DvMF_3149 0.86 -4.4 3,975,111 + -0.8 3,975,278 + DvMF_3150 0.21 -2.5
Or see this region's nucleotide sequence