Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3005 and DvMF_3006 are separated by 108 nucleotides DvMF_3006 and thiE are separated by 5 nucleotides thiE and DvMF_3008 are separated by 227 nucleotides
DvMF_3005: DvMF_3005 - transcriptional regulator, GntR family with aminotransferase domain (RefSeq), at 3,792,047 to 3,793,507
_3005
DvMF_3006: DvMF_3006 - hydroxyethylthiazole kinase (RefSeq), at 3,793,616 to 3,794,419
_3006
DvMF_3007: thiE - thiamine-phosphate pyrophosphorylase (RefSeq), at 3,794,425 to 3,795,066
thiE
DvMF_3008: DvMF_3008 - Dinitrogenase iron-molybdenum cofactor biosynthesis protein (RefSeq), at 3,795,294 to 3,795,629
_3008
Position (kb)
3793
3794
3795 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3792.740 kb on + strand, within DvMF_3005 at 3792.748 kb on - strand, within DvMF_3005 at 3792.782 kb on - strand, within DvMF_3005 at 3793.271 kb on - strand, within DvMF_3005 at 3793.317 kb on - strand, within DvMF_3005 at 3793.347 kb on + strand, within DvMF_3005 at 3793.354 kb on + strand, within DvMF_3005 at 3794.042 kb on - strand, within DvMF_3006 at 3794.198 kb on + strand, within DvMF_3006 at 3794.653 kb on + strand, within thiE at 3794.942 kb on - strand, within thiE at 3794.985 kb on - strand, within thiE at 3795.080 kb on - strand at 3795.129 kb on - strand at 3795.200 kb on + strand at 3795.221 kb on + strand at 3795.221 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 3,792,740 + DvMF_3005 0.47 -1.3 3,792,748 - DvMF_3005 0.48 -0.2 3,792,782 - DvMF_3005 0.50 -0.2 3,793,271 - DvMF_3005 0.84 -0.6 3,793,317 - DvMF_3005 0.87 -1.2 3,793,347 + DvMF_3005 0.89 -0.8 3,793,354 + DvMF_3005 0.89 -0.3 3,794,042 - DvMF_3006 0.53 -3.3 3,794,198 + DvMF_3006 0.72 -1.6 3,794,653 + thiE DvMF_3007 0.36 -0.1 3,794,942 - thiE DvMF_3007 0.81 -0.8 3,794,985 - thiE DvMF_3007 0.87 -3.6 3,795,080 - -0.2 3,795,129 - -0.8 3,795,200 + +0.1 3,795,221 + -0.4 3,795,221 + +0.3
Or see this region's nucleotide sequence