Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2560 and DvMF_2561 are separated by 117 nucleotides DvMF_2561 and DvMF_2562 overlap by 4 nucleotides DvMF_2562 and DvMF_R0070 are separated by 123 nucleotides DvMF_R0070 and DvMF_2563 are separated by 203 nucleotides
DvMF_2560: DvMF_2560 - transcriptional regulator, AraC family (RefSeq), at 3,236,072 to 3,237,073
_2560
DvMF_2561: DvMF_2561 - hypothetical protein (RefSeq), at 3,237,191 to 3,237,337
_2561
DvMF_2562: DvMF_2562 - ThiJ/PfpI domain protein (RefSeq), at 3,237,334 to 3,237,930
_2562
DvMF_R0070: DvMF_R0070 - tRNA-Glu (RefSeq), at 3,238,054 to 3,238,130
_R0070
DvMF_2563: DvMF_2563 - protein of unknown function DUF721 (RefSeq), at 3,238,334 to 3,238,870
_2563
Position (kb)
3237
3238 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3236.293 kb on - strand, within DvMF_2560 at 3236.329 kb on - strand, within DvMF_2560 at 3236.339 kb on - strand, within DvMF_2560 at 3236.429 kb on + strand, within DvMF_2560 at 3236.606 kb on - strand, within DvMF_2560 at 3236.709 kb on + strand, within DvMF_2560 at 3236.828 kb on + strand, within DvMF_2560 at 3236.901 kb on + strand, within DvMF_2560 at 3237.040 kb on + strand at 3237.047 kb on - strand at 3237.331 kb on + strand at 3237.590 kb on - strand, within DvMF_2562 at 3237.826 kb on - strand, within DvMF_2562 at 3238.020 kb on - strand at 3238.104 kb on + strand, within DvMF_R0070
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 3,236,293 - DvMF_2560 0.22 -0.2 3,236,329 - DvMF_2560 0.26 -0.5 3,236,339 - DvMF_2560 0.27 -0.9 3,236,429 + DvMF_2560 0.36 -0.3 3,236,606 - DvMF_2560 0.53 -0.4 3,236,709 + DvMF_2560 0.64 -1.6 3,236,828 + DvMF_2560 0.75 -0.2 3,236,901 + DvMF_2560 0.83 -1.8 3,237,040 + -1.1 3,237,047 - -1.4 3,237,331 + -4.2 3,237,590 - DvMF_2562 0.43 -1.3 3,237,826 - DvMF_2562 0.82 -0.6 3,238,020 - -0.7 3,238,104 + DvMF_R0070 0.65 -0.1
Or see this region's nucleotide sequence