Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2303 and DvMF_2304 are separated by 161 nucleotides DvMF_2304 and hisH overlap by 8 nucleotides hisH and DvMF_2306 are separated by 712 nucleotides
DvMF_2303: DvMF_2303 - esterase/lipase-like protein (RefSeq), at 2,893,323 to 2,894,333
_2303
DvMF_2304: DvMF_2304 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,894,495 to 2,895,274
_2304
DvMF_2305: hisH - IGP synthase, amidotransferase subunit (EC 4.3.2.10) (from data) , at 2,895,267 to 2,895,905
hisH
DvMF_2306: DvMF_2306 - carbamoyl-phosphate synthase, large subunit (RefSeq), at 2,896,618 to 2,899,854
_2306
Position (kb)
2895
2896 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2894.512 kb on + strand at 2894.553 kb on + strand at 2894.615 kb on - strand, within DvMF_2304 at 2894.896 kb on + strand, within DvMF_2304 at 2895.003 kb on + strand, within DvMF_2304 at 2895.005 kb on - strand, within DvMF_2304 at 2895.216 kb on + strand at 2895.282 kb on - strand at 2895.411 kb on + strand, within hisH at 2895.738 kb on - strand, within hisH at 2895.840 kb on - strand, within hisH at 2895.896 kb on - strand at 2895.958 kb on + strand at 2896.166 kb on + strand at 2896.658 kb on + strand at 2896.750 kb on + strand at 2896.750 kb on + strand at 2896.858 kb on + strand at 2896.883 kb on - strand at 2896.900 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 2,894,512 + -1.1 2,894,553 + -2.3 2,894,615 - DvMF_2304 0.15 -2.3 2,894,896 + DvMF_2304 0.51 -3.5 2,895,003 + DvMF_2304 0.65 -2.3 2,895,005 - DvMF_2304 0.65 -4.1 2,895,216 + -2.1 2,895,282 - -0.4 2,895,411 + hisH DvMF_2305 0.23 -2.8 2,895,738 - hisH DvMF_2305 0.74 -3.0 2,895,840 - hisH DvMF_2305 0.90 -1.8 2,895,896 - -2.6 2,895,958 + -1.7 2,896,166 + +1.5 2,896,658 + -0.8 2,896,750 + -0.0 2,896,750 + -3.0 2,896,858 + +0.4 2,896,883 - -0.9 2,896,900 + -0.0
Or see this region's nucleotide sequence