Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2243 and DvMF_2244 are separated by 425 nucleotides DvMF_2244 and DvMF_2245 are separated by 91 nucleotides DvMF_2245 and DvMF_2246 are separated by 196 nucleotides
DvMF_2243: DvMF_2243 - AMP-dependent synthetase and ligase (RefSeq), at 2,816,990 to 2,818,678
_2243
DvMF_2244: DvMF_2244 - Stress responsive alpha-beta barrel domain protein (RefSeq), at 2,819,104 to 2,819,409
_2244
DvMF_2245: DvMF_2245 - Methyltransferase type 11 (RefSeq), at 2,819,501 to 2,820,139
_2245
DvMF_2246: DvMF_2246 - transcriptional regulator, LysR family (RefSeq), at 2,820,336 to 2,821,301
_2246
Position (kb)
2819
2820 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2818.118 kb on + strand, within DvMF_2243 at 2818.808 kb on + strand at 2818.816 kb on - strand at 2818.824 kb on + strand at 2818.994 kb on + strand at 2819.022 kb on + strand at 2819.228 kb on + strand, within DvMF_2244 at 2819.228 kb on + strand, within DvMF_2244 at 2819.243 kb on + strand, within DvMF_2244 at 2819.319 kb on + strand, within DvMF_2244 at 2819.412 kb on - strand at 2819.709 kb on - strand, within DvMF_2245 at 2819.709 kb on - strand, within DvMF_2245 at 2819.719 kb on - strand, within DvMF_2245 at 2820.279 kb on + strand at 2820.312 kb on - strand at 2820.384 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 2,818,118 + DvMF_2243 0.67 -2.8 2,818,808 + -4.4 2,818,816 - -0.7 2,818,824 + -0.4 2,818,994 + -3.0 2,819,022 + -0.7 2,819,228 + DvMF_2244 0.41 -0.8 2,819,228 + DvMF_2244 0.41 -3.3 2,819,243 + DvMF_2244 0.45 -4.0 2,819,319 + DvMF_2244 0.70 -0.8 2,819,412 - +0.9 2,819,709 - DvMF_2245 0.33 -0.6 2,819,709 - DvMF_2245 0.33 -0.9 2,819,719 - DvMF_2245 0.34 -0.7 2,820,279 + +0.2 2,820,312 - -0.7 2,820,384 + -0.2
Or see this region's nucleotide sequence