Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1962 and hisG are separated by 293 nucleotides hisG and hisI overlap by 1 nucleotides hisI and DvMF_1965 are separated by 206 nucleotides
DvMF_1962: DvMF_1962 - shikimate 5-dehydrogenase (RefSeq), at 2,424,917 to 2,425,864
_1962
DvMF_1963: hisG - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 2,426,158 to 2,427,039
hisG
DvMF_1964: hisI - phosphoribosyl-AMP cyclohydrolase (RefSeq), at 2,427,039 to 2,427,440
hisI
DvMF_1965: DvMF_1965 - class II aldolase/adducin family protein (RefSeq), at 2,427,647 to 2,428,804
_1965
Position (kb)
2426
2427
2428 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2426.464 kb on - strand, within hisG at 2426.477 kb on + strand, within hisG at 2426.477 kb on + strand, within hisG at 2426.579 kb on + strand, within hisG at 2426.581 kb on - strand, within hisG at 2426.587 kb on - strand, within hisG at 2426.587 kb on - strand, within hisG at 2426.587 kb on - strand, within hisG at 2426.657 kb on - strand, within hisG at 2426.861 kb on - strand, within hisG at 2427.053 kb on + strand at 2427.131 kb on + strand, within hisI at 2427.303 kb on + strand, within hisI at 2427.436 kb on + strand at 2427.553 kb on + strand at 2427.584 kb on + strand at 2427.592 kb on - strand at 2427.592 kb on - strand at 2427.653 kb on - strand at 2427.658 kb on - strand at 2427.685 kb on + strand at 2427.712 kb on - strand at 2427.729 kb on - strand at 2427.750 kb on + strand at 2427.758 kb on - strand at 2427.763 kb on - strand, within DvMF_1965 at 2427.821 kb on - strand, within DvMF_1965 at 2428.037 kb on + strand, within DvMF_1965
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 2,426,464 - hisG DvMF_1963 0.35 -2.7 2,426,477 + hisG DvMF_1963 0.36 -2.0 2,426,477 + hisG DvMF_1963 0.36 -1.7 2,426,579 + hisG DvMF_1963 0.48 -2.6 2,426,581 - hisG DvMF_1963 0.48 -2.6 2,426,587 - hisG DvMF_1963 0.49 -0.7 2,426,587 - hisG DvMF_1963 0.49 -2.3 2,426,587 - hisG DvMF_1963 0.49 -5.2 2,426,657 - hisG DvMF_1963 0.57 -1.0 2,426,861 - hisG DvMF_1963 0.80 -0.1 2,427,053 + -4.9 2,427,131 + hisI DvMF_1964 0.23 -4.6 2,427,303 + hisI DvMF_1964 0.66 -5.7 2,427,436 + -2.7 2,427,553 + -1.1 2,427,584 + -1.1 2,427,592 - -1.4 2,427,592 - -3.1 2,427,653 - -2.0 2,427,658 - -1.5 2,427,685 + +0.4 2,427,712 - +0.0 2,427,729 - -0.1 2,427,750 + +0.3 2,427,758 - -0.7 2,427,763 - DvMF_1965 0.10 -3.4 2,427,821 - DvMF_1965 0.15 -0.6 2,428,037 + DvMF_1965 0.34 -0.4
Or see this region's nucleotide sequence