Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1762 and DvMF_R0049 are separated by 603 nucleotides DvMF_R0049 and DvMF_1763 are separated by 86 nucleotides DvMF_1763 and DvMF_1764 are separated by 249 nucleotides
DvMF_1762: DvMF_1762 - riboflavin biosynthesis protein RibF (RefSeq), at 2,157,797 to 2,158,741
_1762
DvMF_R0049: DvMF_R0049 - tRNA-Leu (RefSeq), at 2,159,345 to 2,159,431
_R0049
DvMF_1763: DvMF_1763 - sigma54 specific transcriptional regulator, Fis family (RefSeq), at 2,159,518 to 2,160,684
_1763
DvMF_1764: DvMF_1764 - protein of unknown function DUF81 (RefSeq), at 2,160,934 to 2,161,911
_1764
Position (kb)
2159
2160
2161 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2158.862 kb on - strand at 2159.045 kb on + strand at 2159.112 kb on + strand at 2159.136 kb on + strand at 2159.139 kb on - strand at 2159.139 kb on - strand at 2159.181 kb on + strand at 2159.188 kb on + strand at 2159.223 kb on + strand at 2159.416 kb on - strand, within DvMF_R0049 at 2160.133 kb on - strand, within DvMF_1763 at 2160.331 kb on + strand, within DvMF_1763 at 2160.331 kb on + strand, within DvMF_1763 at 2160.339 kb on - strand, within DvMF_1763 at 2160.355 kb on + strand, within DvMF_1763 at 2160.355 kb on + strand, within DvMF_1763 at 2160.405 kb on - strand, within DvMF_1763
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 2,158,862 - -1.2 2,159,045 + -4.5 2,159,112 + -1.8 2,159,136 + -0.8 2,159,139 - -4.9 2,159,139 - -0.5 2,159,181 + -0.8 2,159,188 + +0.9 2,159,223 + -0.0 2,159,416 - DvMF_R0049 0.82 +0.0 2,160,133 - DvMF_1763 0.53 -2.8 2,160,331 + DvMF_1763 0.70 -3.2 2,160,331 + DvMF_1763 0.70 -2.9 2,160,339 - DvMF_1763 0.70 -1.4 2,160,355 + DvMF_1763 0.72 -2.3 2,160,355 + DvMF_1763 0.72 +0.0 2,160,405 - DvMF_1763 0.76 -0.7
Or see this region's nucleotide sequence