Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpA and DvMF_1741 overlap by 4 nucleotides DvMF_1741 and DvMF_1742 are separated by 39 nucleotides DvMF_1742 and DvMF_1743 overlap by 4 nucleotides
DvMF_1740: trpA - tryptophan synthase subunit alpha (RefSeq), at 2,127,525 to 2,128,337
trpA
DvMF_1741: DvMF_1741 - tryptophan synthase subunit beta (RefSeq), at 2,128,334 to 2,129,518
_1741
DvMF_1742: DvMF_1742 - Phosphoribosylanthranilate isomerase (RefSeq), at 2,129,558 to 2,130,235
_1742
DvMF_1743: DvMF_1743 - Indole-3-glycerol-phosphate synthase (RefSeq), at 2,130,232 to 2,131,197
_1743
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2127.519 kb on - strand at 2127.524 kb on - strand at 2127.747 kb on + strand, within trpA at 2128.203 kb on + strand, within trpA at 2128.412 kb on - strand at 2128.451 kb on - strand at 2128.583 kb on - strand, within DvMF_1741 at 2128.616 kb on - strand, within DvMF_1741 at 2128.646 kb on + strand, within DvMF_1741 at 2128.703 kb on - strand, within DvMF_1741 at 2128.726 kb on + strand, within DvMF_1741 at 2128.951 kb on - strand, within DvMF_1741 at 2128.973 kb on - strand, within DvMF_1741 at 2129.072 kb on + strand, within DvMF_1741 at 2129.092 kb on + strand, within DvMF_1741 at 2129.248 kb on + strand, within DvMF_1741 at 2129.251 kb on - strand, within DvMF_1741 at 2129.335 kb on + strand, within DvMF_1741 at 2129.368 kb on + strand, within DvMF_1741 at 2129.528 kb on - strand at 2129.646 kb on + strand, within DvMF_1742 at 2129.666 kb on + strand, within DvMF_1742 at 2129.865 kb on + strand, within DvMF_1742 at 2130.138 kb on + strand, within DvMF_1742 at 2130.178 kb on + strand at 2130.405 kb on - strand, within DvMF_1743 at 2130.452 kb on - strand, within DvMF_1743
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 2,127,519 - -1.4 2,127,524 - -0.5 2,127,747 + trpA DvMF_1740 0.27 -0.1 2,128,203 + trpA DvMF_1740 0.83 -1.1 2,128,412 - -3.3 2,128,451 - +0.5 2,128,583 - DvMF_1741 0.21 -1.2 2,128,616 - DvMF_1741 0.24 -3.3 2,128,646 + DvMF_1741 0.26 -1.4 2,128,703 - DvMF_1741 0.31 -2.2 2,128,726 + DvMF_1741 0.33 -1.0 2,128,951 - DvMF_1741 0.52 -1.3 2,128,973 - DvMF_1741 0.54 -0.7 2,129,072 + DvMF_1741 0.62 -1.1 2,129,092 + DvMF_1741 0.64 -2.0 2,129,248 + DvMF_1741 0.77 -1.1 2,129,251 - DvMF_1741 0.77 -1.1 2,129,335 + DvMF_1741 0.84 -2.1 2,129,368 + DvMF_1741 0.87 -0.2 2,129,528 - -0.6 2,129,646 + DvMF_1742 0.13 -2.0 2,129,666 + DvMF_1742 0.16 -1.2 2,129,865 + DvMF_1742 0.45 -2.0 2,130,138 + DvMF_1742 0.86 -0.1 2,130,178 + -0.6 2,130,405 - DvMF_1743 0.18 -0.4 2,130,452 - DvMF_1743 0.23 -2.6
Or see this region's nucleotide sequence