Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplM and rpsI are separated by 22 nucleotides rpsI and aroK are separated by 259 nucleotides aroK and DvMF_0972 are separated by 1 nucleotides DvMF_0972 and DvMF_0973 are separated by 118 nucleotides
DvMF_0969: rplM - 50S ribosomal protein L13 (RefSeq), at 1,176,576 to 1,177,010
rplM
DvMF_0970: rpsI - 30S ribosomal protein S9 (RefSeq), at 1,177,033 to 1,177,425
rpsI
DvMF_0971: aroK - Homoserine kinase (EC:2.7.1.39) <small>(from data)</small>, at 1,177,685 to 1,178,242
aroK
DvMF_0972: DvMF_0972 - hypothetical protein (RefSeq), at 1,178,244 to 1,178,981
_0972
DvMF_0973: DvMF_0973 - putative lipoprotein (RefSeq), at 1,179,100 to 1,179,660
_0973
Position (kb) 1177
1178
1179 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1177.553 kb on - strand at 1177.772 kb on + strand, within aroK at 1177.891 kb on - strand, within aroK at 1177.969 kb on - strand, within aroK at 1178.494 kb on + strand, within DvMF_0972 at 1178.879 kb on + strand, within DvMF_0972 at 1179.218 kb on + strand, within DvMF_0973
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 1,177,553 - +0.1 1,177,772 + aroK DvMF_0971 0.16 -5.4 1,177,891 - aroK DvMF_0971 0.37 -2.4 1,177,969 - aroK DvMF_0971 0.51 -2.8 1,178,494 + DvMF_0972 0.34 -0.6 1,178,879 + DvMF_0972 0.86 -0.4 1,179,218 + DvMF_0973 0.21 -0.3
Or see this region's nucleotide sequence