Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0344 and DvMF_0345 are separated by 124 nucleotides DvMF_0345 and DvMF_0346 are separated by 74 nucleotides DvMF_0346 and DvMF_0347 are separated by 163 nucleotides
DvMF_0344: DvMF_0344 - rod shape-determining protein MreB (RefSeq), at 403,148 to 404,170
_0344
DvMF_0345: DvMF_0345 - Inositol-phosphate phosphatase (RefSeq), at 404,295 to 405,161
_0345
DvMF_0346: DvMF_0346 - NUDIX hydrolase (RefSeq), at 405,236 to 405,784
_0346
DvMF_0347: DvMF_0347 - ribosomal-protein-alanine acetyltransferase (RefSeq), at 405,948 to 406,526
_0347
Position (kb) 404
405
406 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 403.649 kb on - strand, within DvMF_0344 at 404.120 kb on + strand at 404.151 kb on + strand at 404.206 kb on + strand at 404.861 kb on - strand, within DvMF_0345 at 405.204 kb on + strand at 405.234 kb on + strand at 405.242 kb on - strand at 405.315 kb on + strand, within DvMF_0346 at 405.336 kb on + strand, within DvMF_0346 at 405.618 kb on + strand, within DvMF_0346 at 405.618 kb on + strand, within DvMF_0346 at 405.683 kb on + strand, within DvMF_0346 at 405.691 kb on - strand, within DvMF_0346 at 405.809 kb on - strand at 405.809 kb on - strand at 405.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 403,649 - DvMF_0344 0.49 -0.7 404,120 + +0.7 404,151 + +1.2 404,206 + -0.8 404,861 - DvMF_0345 0.65 -1.6 405,204 + -1.6 405,234 + -1.5 405,242 - -0.6 405,315 + DvMF_0346 0.14 -0.9 405,336 + DvMF_0346 0.18 -1.0 405,618 + DvMF_0346 0.70 -1.0 405,618 + DvMF_0346 0.70 -4.3 405,683 + DvMF_0346 0.81 -0.8 405,691 - DvMF_0346 0.83 -0.9 405,809 - -0.7 405,809 - -1.3 405,953 + -0.7
Or see this region's nucleotide sequence