Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0179 and DvMF_0180 are separated by 267 nucleotides DvMF_0180 and DvMF_R0010 are separated by 304 nucleotides DvMF_R0010 and DvMF_R0011 are separated by 77 nucleotides DvMF_R0011 and DvMF_R0012 are separated by 61 nucleotides
DvMF_0179: DvMF_0179 - hypothetical protein (RefSeq), at 195,652 to 196,503
_0179
DvMF_0180: DvMF_0180 - hypothetical protein (RefSeq), at 196,771 to 197,655
_0180
DvMF_R0010: DvMF_R0010 - tRNA-Arg (RefSeq), at 197,960 to 198,036
_R0010
DvMF_R0011: DvMF_R0011 - tRNA-Arg (RefSeq), at 198,114 to 198,190
_R0011
DvMF_R0012: DvMF_R0012 - tRNA-Arg (RefSeq), at 198,252 to 198,328
_R0012
Position (kb) 196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 195.871 kb on + strand, within DvMF_0179 at 196.162 kb on - strand, within DvMF_0179 at 196.177 kb on + strand, within DvMF_0179 at 196.185 kb on - strand, within DvMF_0179 at 196.492 kb on + strand at 196.547 kb on + strand at 196.692 kb on + strand at 196.700 kb on - strand at 196.790 kb on + strand at 196.798 kb on - strand at 196.915 kb on - strand, within DvMF_0180 at 196.920 kb on - strand, within DvMF_0180 at 197.269 kb on + strand, within DvMF_0180 at 197.454 kb on + strand, within DvMF_0180 at 197.472 kb on - strand, within DvMF_0180 at 197.472 kb on - strand, within DvMF_0180 at 197.518 kb on + strand, within DvMF_0180 at 197.518 kb on + strand, within DvMF_0180 at 197.718 kb on - strand at 198.531 kb on - strand at 198.578 kb on + strand at 198.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 195,871 + DvMF_0179 0.26 -3.8 196,162 - DvMF_0179 0.60 -0.2 196,177 + DvMF_0179 0.62 -1.8 196,185 - DvMF_0179 0.63 -1.2 196,492 + -0.2 196,547 + -1.2 196,692 + -1.3 196,700 - -1.3 196,790 + -0.5 196,798 - -0.8 196,915 - DvMF_0180 0.16 -0.8 196,920 - DvMF_0180 0.17 -2.1 197,269 + DvMF_0180 0.56 -0.4 197,454 + DvMF_0180 0.77 +0.9 197,472 - DvMF_0180 0.79 +0.4 197,472 - DvMF_0180 0.79 -1.1 197,518 + DvMF_0180 0.84 -0.7 197,518 + DvMF_0180 0.84 +0.2 197,718 - -1.1 198,531 - -0.7 198,578 + -2.0 198,579 - -0.2
Or see this region's nucleotide sequence