Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0177 and DvMF_0178 are separated by 166 nucleotides DvMF_0178 and DvMF_0179 are separated by 300 nucleotides DvMF_0179 and DvMF_0180 are separated by 267 nucleotides
DvMF_0177: DvMF_0177 - Lytic transglycosylase catalytic (RefSeq), at 193,386 to 194,675
_0177
DvMF_0178: DvMF_0178 - outer membrane chaperone Skp (OmpH) (RefSeq), at 194,842 to 195,351
_0178
DvMF_0179: DvMF_0179 - hypothetical protein (RefSeq), at 195,652 to 196,503
_0179
DvMF_0180: DvMF_0180 - hypothetical protein (RefSeq), at 196,771 to 197,655
_0180
Position (kb) 195
196
197 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 194.669 kb on + strand at 194.736 kb on - strand at 194.929 kb on + strand, within DvMF_0178 at 194.986 kb on - strand, within DvMF_0178 at 195.132 kb on + strand, within DvMF_0178 at 195.470 kb on + strand at 195.470 kb on + strand at 195.531 kb on - strand at 195.547 kb on + strand at 195.552 kb on + strand at 195.770 kb on - strand, within DvMF_0179 at 195.871 kb on + strand, within DvMF_0179 at 196.162 kb on - strand, within DvMF_0179 at 196.177 kb on + strand, within DvMF_0179 at 196.185 kb on - strand, within DvMF_0179 at 196.492 kb on + strand at 196.547 kb on + strand at 196.692 kb on + strand at 196.700 kb on - strand at 196.790 kb on + strand at 196.798 kb on - strand at 196.915 kb on - strand, within DvMF_0180 at 196.920 kb on - strand, within DvMF_0180 at 197.269 kb on + strand, within DvMF_0180 at 197.454 kb on + strand, within DvMF_0180 at 197.472 kb on - strand, within DvMF_0180 at 197.472 kb on - strand, within DvMF_0180
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 194,669 + -1.2 194,736 - +0.0 194,929 + DvMF_0178 0.17 -0.7 194,986 - DvMF_0178 0.28 -4.1 195,132 + DvMF_0178 0.57 -0.5 195,470 + -1.1 195,470 + -1.3 195,531 - -2.4 195,547 + -0.2 195,552 + -0.3 195,770 - DvMF_0179 0.14 -2.1 195,871 + DvMF_0179 0.26 -3.8 196,162 - DvMF_0179 0.60 -0.2 196,177 + DvMF_0179 0.62 -1.8 196,185 - DvMF_0179 0.63 -1.2 196,492 + -0.2 196,547 + -1.2 196,692 + -1.3 196,700 - -1.3 196,790 + -0.5 196,798 - -0.8 196,915 - DvMF_0180 0.16 -0.8 196,920 - DvMF_0180 0.17 -2.1 197,269 + DvMF_0180 0.56 -0.4 197,454 + DvMF_0180 0.77 +0.9 197,472 - DvMF_0180 0.79 +0.4 197,472 - DvMF_0180 0.79 -1.1
Or see this region's nucleotide sequence