Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0149 and DvMF_0150 are separated by 9 nucleotides DvMF_0150 and DvMF_0151 overlap by 14 nucleotides DvMF_0151 and DvMF_0152 are separated by 29 nucleotides DvMF_0152 and DvMF_0153 are separated by 0 nucleotides
DvMF_0149: DvMF_0149 - protein of unknown function YGGT (RefSeq), at 165,865 to 166,167
_0149
DvMF_0150: DvMF_0150 - DivIVA family protein (RefSeq), at 166,177 to 166,686
_0150
DvMF_0151: DvMF_0151 - protein of unknown function DUF167 (RefSeq), at 166,673 to 166,993
_0151
DvMF_0152: DvMF_0152 - hypothetical protein (RefSeq), at 167,023 to 167,253
_0152
DvMF_0153: DvMF_0153 - acetolactate synthase, large subunit, biosynthetic type (RefSeq), at 167,254 to 168,942
_0153
Position (kb) 166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 165.743 kb on + strand at 165.743 kb on + strand at 165.743 kb on + strand at 166.078 kb on - strand, within DvMF_0149 at 166.249 kb on - strand, within DvMF_0150 at 166.452 kb on - strand, within DvMF_0150 at 166.500 kb on + strand, within DvMF_0150 at 166.630 kb on + strand, within DvMF_0150 at 166.823 kb on + strand, within DvMF_0151 at 167.247 kb on - strand at 167.302 kb on + strand at 167.442 kb on + strand, within DvMF_0153 at 167.476 kb on - strand, within DvMF_0153
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 165,743 + +0.1 165,743 + -0.7 165,743 + -3.7 166,078 - DvMF_0149 0.70 +0.9 166,249 - DvMF_0150 0.14 -0.1 166,452 - DvMF_0150 0.54 -2.7 166,500 + DvMF_0150 0.63 -0.9 166,630 + DvMF_0150 0.89 -0.9 166,823 + DvMF_0151 0.47 -0.0 167,247 - -0.5 167,302 + -0.6 167,442 + DvMF_0153 0.11 +0.3 167,476 - DvMF_0153 0.13 -0.5
Or see this region's nucleotide sequence