Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0047 and DvMF_0048 are separated by 162 nucleotides DvMF_0048 and DvMF_0049 are separated by 264 nucleotides
DvMF_0047: DvMF_0047 - argininosuccinate synthase (RefSeq), at 62,630 to 63,835
_0047
DvMF_0048: DvMF_0048 - argininosuccinate lyase (RefSeq), at 63,998 to 65,380
_0048
DvMF_0049: DvMF_0049 - hypothetical protein (RefSeq), at 65,645 to 66,274
_0049
Position (kb) 63
64
65
66 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 63.126 kb on + strand, within DvMF_0047 at 63.144 kb on + strand, within DvMF_0047 at 63.144 kb on + strand, within DvMF_0047 at 63.154 kb on + strand, within DvMF_0047 at 63.154 kb on + strand, within DvMF_0047 at 63.154 kb on + strand, within DvMF_0047 at 63.157 kb on - strand, within DvMF_0047 at 63.162 kb on - strand, within DvMF_0047 at 63.172 kb on - strand, within DvMF_0047 at 63.455 kb on + strand, within DvMF_0047 at 63.468 kb on - strand, within DvMF_0047 at 63.664 kb on + strand, within DvMF_0047 at 63.672 kb on - strand, within DvMF_0047 at 63.672 kb on - strand, within DvMF_0047 at 63.708 kb on - strand, within DvMF_0047 at 64.097 kb on + strand at 64.102 kb on + strand at 64.646 kb on + strand, within DvMF_0048 at 64.852 kb on + strand, within DvMF_0048 at 64.972 kb on + strand, within DvMF_0048 at 65.213 kb on + strand, within DvMF_0048 at 65.331 kb on + strand at 65.507 kb on - strand at 65.667 kb on + strand at 65.700 kb on - strand at 65.818 kb on + strand, within DvMF_0049 at 65.904 kb on + strand, within DvMF_0049 at 66.116 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 63,126 + DvMF_0047 0.41 -5.3 63,144 + DvMF_0047 0.43 -2.1 63,144 + DvMF_0047 0.43 -1.6 63,154 + DvMF_0047 0.43 -2.8 63,154 + DvMF_0047 0.43 -0.7 63,154 + DvMF_0047 0.43 -3.0 63,157 - DvMF_0047 0.44 -3.6 63,162 - DvMF_0047 0.44 -2.2 63,172 - DvMF_0047 0.45 -3.2 63,455 + DvMF_0047 0.68 -0.5 63,468 - DvMF_0047 0.69 -2.7 63,664 + DvMF_0047 0.86 -0.9 63,672 - DvMF_0047 0.86 -2.8 63,672 - DvMF_0047 0.86 -2.0 63,708 - DvMF_0047 0.89 -3.8 64,097 + -1.2 64,102 + -0.3 64,646 + DvMF_0048 0.47 -1.7 64,852 + DvMF_0048 0.62 -3.3 64,972 + DvMF_0048 0.70 -2.8 65,213 + DvMF_0048 0.88 -2.9 65,331 + -4.2 65,507 - -0.9 65,667 + -0.7 65,700 - -1.8 65,818 + DvMF_0049 0.27 -0.9 65,904 + DvMF_0049 0.41 -0.5 66,116 + DvMF_0049 0.75 -0.1 66,136 + DvMF_0049 0.78 -0.2 66,136 + DvMF_0049 0.78 -0.8 66,271 - -1.5
Or see this region's nucleotide sequence