Experiment: MoLS4 with D-Cycloserine 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0046 and DvMF_0047 are separated by 68 nucleotides DvMF_0047 and DvMF_0048 are separated by 162 nucleotides
DvMF_0046: DvMF_0046 - ornithine carbamoyltransferase (RefSeq), at 61,659 to 62,561
_0046
DvMF_0047: DvMF_0047 - argininosuccinate synthase (RefSeq), at 62,630 to 63,835
_0047
DvMF_0048: DvMF_0048 - argininosuccinate lyase (RefSeq), at 63,998 to 65,380
_0048
Position (kb) 62
63
64 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 61.762 kb on - strand, within DvMF_0046 at 61.852 kb on - strand, within DvMF_0046 at 61.905 kb on - strand, within DvMF_0046 at 61.960 kb on + strand, within DvMF_0046 at 62.083 kb on + strand, within DvMF_0046 at 62.180 kb on + strand, within DvMF_0046 at 62.180 kb on + strand, within DvMF_0046 at 62.180 kb on + strand, within DvMF_0046 at 62.188 kb on - strand, within DvMF_0046 at 62.188 kb on - strand, within DvMF_0046 at 62.263 kb on + strand, within DvMF_0046 at 62.280 kb on + strand, within DvMF_0046 at 62.617 kb on + strand at 62.741 kb on - strand at 62.945 kb on + strand, within DvMF_0047 at 63.126 kb on + strand, within DvMF_0047 at 63.144 kb on + strand, within DvMF_0047 at 63.144 kb on + strand, within DvMF_0047 at 63.154 kb on + strand, within DvMF_0047 at 63.154 kb on + strand, within DvMF_0047 at 63.154 kb on + strand, within DvMF_0047 at 63.157 kb on - strand, within DvMF_0047 at 63.162 kb on - strand, within DvMF_0047 at 63.172 kb on - strand, within DvMF_0047 at 63.455 kb on + strand, within DvMF_0047 at 63.468 kb on - strand, within DvMF_0047 at 63.664 kb on + strand, within DvMF_0047 at 63.672 kb on - strand, within DvMF_0047 at 63.672 kb on - strand, within DvMF_0047 at 63.708 kb on - strand, within DvMF_0047 at 64.097 kb on + strand at 64.102 kb on + strand at 64.646 kb on + strand, within DvMF_0048
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.0625 mg/ml remove 61,762 - DvMF_0046 0.11 -1.5 61,852 - DvMF_0046 0.21 -1.2 61,905 - DvMF_0046 0.27 -4.4 61,960 + DvMF_0046 0.33 -5.0 62,083 + DvMF_0046 0.47 -1.0 62,180 + DvMF_0046 0.58 -3.5 62,180 + DvMF_0046 0.58 -3.9 62,180 + DvMF_0046 0.58 -2.0 62,188 - DvMF_0046 0.59 -2.0 62,188 - DvMF_0046 0.59 -4.1 62,263 + DvMF_0046 0.67 +0.3 62,280 + DvMF_0046 0.69 -4.8 62,617 + -1.3 62,741 - -3.9 62,945 + DvMF_0047 0.26 -3.5 63,126 + DvMF_0047 0.41 -5.3 63,144 + DvMF_0047 0.43 -2.1 63,144 + DvMF_0047 0.43 -1.6 63,154 + DvMF_0047 0.43 -2.8 63,154 + DvMF_0047 0.43 -0.7 63,154 + DvMF_0047 0.43 -3.0 63,157 - DvMF_0047 0.44 -3.6 63,162 - DvMF_0047 0.44 -2.2 63,172 - DvMF_0047 0.45 -3.2 63,455 + DvMF_0047 0.68 -0.5 63,468 - DvMF_0047 0.69 -2.7 63,664 + DvMF_0047 0.86 -0.9 63,672 - DvMF_0047 0.86 -2.8 63,672 - DvMF_0047 0.86 -2.0 63,708 - DvMF_0047 0.89 -3.8 64,097 + -1.2 64,102 + -0.3 64,646 + DvMF_0048 0.47 -1.7
Or see this region's nucleotide sequence