Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_2735 and Sama_2736 are separated by 200 nucleotides Sama_2736 and Sama_2737 are separated by 79 nucleotides Sama_2737 and Sama_2738 are separated by 87 nucleotides Sama_2738 and Sama_2739 are separated by 139 nucleotides
Sama_2735: Sama_2735 - hypothetical protein (RefSeq), at 3,268,191 to 3,269,120
_2735
Sama_2736: Sama_2736 - ribose-5-phosphate isomerase A (RefSeq), at 3,269,321 to 3,269,980
_2736
Sama_2737: Sama_2737 - single-stranded-DNA-specific exonuclease RecJ (RefSeq), at 3,270,060 to 3,271,784
_2737
Sama_2738: Sama_2738 - thiol:disulfide interchange protein DsbC (RefSeq), at 3,271,872 to 3,272,600
_2738
Sama_2739: Sama_2739 - integrase/recombinase XerD (RefSeq), at 3,272,740 to 3,273,705
_2739
Position (kb)
3270
3271
3272 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3269.084 kb on + strand at 3269.085 kb on - strand at 3269.085 kb on - strand at 3269.094 kb on + strand at 3269.094 kb on + strand at 3269.094 kb on + strand at 3269.120 kb on + strand at 3269.164 kb on - strand at 3269.213 kb on + strand at 3269.214 kb on - strand at 3269.273 kb on + strand at 3269.274 kb on - strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.275 kb on + strand at 3269.276 kb on - strand at 3269.278 kb on + strand at 3269.981 kb on + strand at 3269.981 kb on + strand at 3269.982 kb on - strand at 3269.986 kb on + strand at 3269.986 kb on + strand at 3269.987 kb on - strand at 3270.176 kb on + strand at 3270.176 kb on + strand at 3270.177 kb on - strand at 3270.244 kb on - strand, within Sama_2737 at 3270.244 kb on - strand, within Sama_2737 at 3270.273 kb on + strand, within Sama_2737 at 3270.273 kb on + strand, within Sama_2737 at 3270.273 kb on + strand, within Sama_2737 at 3270.274 kb on - strand, within Sama_2737 at 3270.274 kb on - strand, within Sama_2737 at 3270.274 kb on - strand, within Sama_2737 at 3270.274 kb on - strand, within Sama_2737 at 3270.274 kb on - strand, within Sama_2737 at 3270.341 kb on + strand, within Sama_2737 at 3270.368 kb on - strand, within Sama_2737 at 3270.528 kb on - strand, within Sama_2737 at 3270.572 kb on + strand, within Sama_2737 at 3270.573 kb on - strand, within Sama_2737 at 3270.573 kb on - strand, within Sama_2737 at 3270.606 kb on + strand, within Sama_2737 at 3270.606 kb on + strand, within Sama_2737 at 3270.607 kb on - strand, within Sama_2737 at 3270.652 kb on + strand, within Sama_2737 at 3270.652 kb on + strand, within Sama_2737 at 3270.652 kb on + strand, within Sama_2737 at 3270.652 kb on + strand, within Sama_2737 at 3270.652 kb on + strand, within Sama_2737 at 3270.652 kb on + strand, within Sama_2737 at 3270.653 kb on - strand, within Sama_2737 at 3270.653 kb on - strand, within Sama_2737 at 3270.653 kb on - strand, within Sama_2737 at 3270.684 kb on + strand, within Sama_2737 at 3270.685 kb on - strand, within Sama_2737 at 3270.685 kb on - strand, within Sama_2737 at 3270.713 kb on - strand, within Sama_2737 at 3270.720 kb on + strand, within Sama_2737 at 3270.721 kb on - strand, within Sama_2737 at 3270.721 kb on - strand, within Sama_2737 at 3270.721 kb on - strand, within Sama_2737 at 3270.771 kb on + strand, within Sama_2737 at 3270.772 kb on - strand, within Sama_2737 at 3270.818 kb on + strand, within Sama_2737 at 3270.818 kb on + strand, within Sama_2737 at 3270.818 kb on + strand, within Sama_2737 at 3270.853 kb on + strand, within Sama_2737 at 3270.853 kb on + strand, within Sama_2737 at 3270.853 kb on + strand, within Sama_2737 at 3270.853 kb on + strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.854 kb on - strand, within Sama_2737 at 3270.974 kb on + strand, within Sama_2737 at 3271.037 kb on + strand, within Sama_2737 at 3271.038 kb on - strand, within Sama_2737 at 3271.038 kb on - strand, within Sama_2737 at 3271.038 kb on - strand, within Sama_2737 at 3271.107 kb on + strand, within Sama_2737 at 3271.107 kb on + strand, within Sama_2737 at 3271.107 kb on + strand, within Sama_2737 at 3271.108 kb on - strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.110 kb on + strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.111 kb on - strand, within Sama_2737 at 3271.144 kb on - strand, within Sama_2737 at 3271.144 kb on - strand, within Sama_2737 at 3271.144 kb on - strand, within Sama_2737 at 3271.145 kb on + strand, within Sama_2737 at 3271.145 kb on + strand, within Sama_2737 at 3271.146 kb on - strand, within Sama_2737 at 3271.146 kb on - strand, within Sama_2737 at 3271.204 kb on + strand, within Sama_2737 at 3271.204 kb on + strand, within Sama_2737 at 3271.204 kb on + strand, within Sama_2737 at 3271.268 kb on + strand, within Sama_2737 at 3271.269 kb on - strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.272 kb on + strand, within Sama_2737 at 3271.273 kb on - strand, within Sama_2737 at 3271.377 kb on - strand, within Sama_2737 at 3271.395 kb on - strand, within Sama_2737 at 3271.395 kb on - strand, within Sama_2737 at 3271.456 kb on + strand, within Sama_2737 at 3271.476 kb on - strand, within Sama_2737 at 3271.476 kb on - strand, within Sama_2737 at 3271.497 kb on - strand, within Sama_2737 at 3271.574 kb on + strand, within Sama_2737 at 3271.574 kb on + strand, within Sama_2737 at 3271.574 kb on + strand, within Sama_2737 at 3271.575 kb on - strand, within Sama_2737 at 3271.575 kb on - strand, within Sama_2737 at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.634 kb on + strand at 3271.635 kb on - strand at 3271.635 kb on - strand at 3271.635 kb on - strand at 3271.687 kb on - strand at 3271.714 kb on - strand at 3271.714 kb on - strand at 3271.777 kb on + strand at 3271.777 kb on + strand at 3271.777 kb on + strand at 3271.777 kb on + strand at 3271.778 kb on - strand at 3271.778 kb on - strand at 3271.821 kb on + strand at 3271.821 kb on + strand at 3271.821 kb on + strand at 3271.821 kb on + strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.822 kb on - strand at 3271.873 kb on + strand at 3271.977 kb on + strand, within Sama_2738 at 3271.978 kb on - strand, within Sama_2738 at 3272.002 kb on + strand, within Sama_2738 at 3272.003 kb on - strand, within Sama_2738 at 3272.131 kb on - strand, within Sama_2738 at 3272.131 kb on - strand, within Sama_2738 at 3272.181 kb on - strand, within Sama_2738 at 3272.182 kb on + strand, within Sama_2738 at 3272.183 kb on - strand, within Sama_2738 at 3272.183 kb on - strand, within Sama_2738 at 3272.215 kb on + strand, within Sama_2738 at 3272.271 kb on - strand, within Sama_2738 at 3272.271 kb on - strand, within Sama_2738 at 3272.368 kb on + strand, within Sama_2738 at 3272.369 kb on - strand, within Sama_2738 at 3272.410 kb on + strand, within Sama_2738 at 3272.410 kb on + strand, within Sama_2738 at 3272.411 kb on - strand, within Sama_2738 at 3272.411 kb on - strand, within Sama_2738 at 3272.629 kb on + strand at 3272.630 kb on - strand at 3272.630 kb on - strand at 3272.630 kb on - strand at 3272.641 kb on + strand at 3272.643 kb on + strand at 3272.643 kb on + strand at 3272.643 kb on + strand at 3272.643 kb on + strand at 3272.644 kb on - strand at 3272.644 kb on - strand at 3272.644 kb on - strand at 3272.644 kb on - strand at 3272.644 kb on - strand at 3272.696 kb on + strand at 3272.697 kb on - strand at 3272.714 kb on + strand at 3272.714 kb on + strand at 3272.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,269,084 + -1.7 3,269,085 - +2.2 3,269,085 - -1.7 3,269,094 + +2.1 3,269,094 + -1.3 3,269,094 + -1.2 3,269,120 + -1.2 3,269,164 - +1.2 3,269,213 + -0.3 3,269,214 - +0.6 3,269,273 + +0.1 3,269,274 - +1.8 3,269,275 + -1.9 3,269,275 + +0.1 3,269,275 + +0.8 3,269,275 + -2.7 3,269,275 + -1.7 3,269,275 + -2.2 3,269,275 + -1.0 3,269,275 + +1.7 3,269,276 - -2.1 3,269,278 + -1.2 3,269,981 + -1.1 3,269,981 + +1.8 3,269,982 - -0.5 3,269,986 + +2.2 3,269,986 + -0.5 3,269,987 - +1.6 3,270,176 + -1.2 3,270,176 + -0.2 3,270,177 - +2.2 3,270,244 - Sama_2737 0.11 +0.8 3,270,244 - Sama_2737 0.11 -2.7 3,270,273 + Sama_2737 0.12 +1.1 3,270,273 + Sama_2737 0.12 -0.3 3,270,273 + Sama_2737 0.12 -3.4 3,270,274 - Sama_2737 0.12 +1.5 3,270,274 - Sama_2737 0.12 -2.4 3,270,274 - Sama_2737 0.12 -1.0 3,270,274 - Sama_2737 0.12 -0.1 3,270,274 - Sama_2737 0.12 -1.5 3,270,341 + Sama_2737 0.16 -2.8 3,270,368 - Sama_2737 0.18 -0.3 3,270,528 - Sama_2737 0.27 +0.2 3,270,572 + Sama_2737 0.30 +0.4 3,270,573 - Sama_2737 0.30 -1.9 3,270,573 - Sama_2737 0.30 -0.6 3,270,606 + Sama_2737 0.32 +2.1 3,270,606 + Sama_2737 0.32 -2.6 3,270,607 - Sama_2737 0.32 -2.2 3,270,652 + Sama_2737 0.34 +2.3 3,270,652 + Sama_2737 0.34 +0.5 3,270,652 + Sama_2737 0.34 +1.5 3,270,652 + Sama_2737 0.34 +1.1 3,270,652 + Sama_2737 0.34 -1.1 3,270,652 + Sama_2737 0.34 +1.1 3,270,653 - Sama_2737 0.34 -2.3 3,270,653 - Sama_2737 0.34 -1.0 3,270,653 - Sama_2737 0.34 +2.4 3,270,684 + Sama_2737 0.36 -0.5 3,270,685 - Sama_2737 0.36 -1.9 3,270,685 - Sama_2737 0.36 -2.1 3,270,713 - Sama_2737 0.38 -0.9 3,270,720 + Sama_2737 0.38 -1.2 3,270,721 - Sama_2737 0.38 -1.4 3,270,721 - Sama_2737 0.38 +0.7 3,270,721 - Sama_2737 0.38 -1.0 3,270,771 + Sama_2737 0.41 -0.5 3,270,772 - Sama_2737 0.41 -1.9 3,270,818 + Sama_2737 0.44 +0.1 3,270,818 + Sama_2737 0.44 -2.4 3,270,818 + Sama_2737 0.44 -1.5 3,270,853 + Sama_2737 0.46 +0.8 3,270,853 + Sama_2737 0.46 -1.7 3,270,853 + Sama_2737 0.46 +4.1 3,270,853 + Sama_2737 0.46 +2.0 3,270,854 - Sama_2737 0.46 -0.5 3,270,854 - Sama_2737 0.46 +1.7 3,270,854 - Sama_2737 0.46 -3.4 3,270,854 - Sama_2737 0.46 -0.5 3,270,854 - Sama_2737 0.46 -1.9 3,270,854 - Sama_2737 0.46 -1.1 3,270,854 - Sama_2737 0.46 -1.7 3,270,974 + Sama_2737 0.53 -0.7 3,271,037 + Sama_2737 0.57 +0.8 3,271,038 - Sama_2737 0.57 -0.5 3,271,038 - Sama_2737 0.57 +1.2 3,271,038 - Sama_2737 0.57 +0.9 3,271,107 + Sama_2737 0.61 -1.9 3,271,107 + Sama_2737 0.61 -0.6 3,271,107 + Sama_2737 0.61 +0.4 3,271,108 - Sama_2737 0.61 -1.5 3,271,110 + Sama_2737 0.61 -3.1 3,271,110 + Sama_2737 0.61 +1.1 3,271,110 + Sama_2737 0.61 -0.9 3,271,110 + Sama_2737 0.61 -2.7 3,271,110 + Sama_2737 0.61 -1.7 3,271,110 + Sama_2737 0.61 +2.7 3,271,110 + Sama_2737 0.61 -0.5 3,271,110 + Sama_2737 0.61 +0.7 3,271,110 + Sama_2737 0.61 -1.5 3,271,110 + Sama_2737 0.61 -2.4 3,271,110 + Sama_2737 0.61 +0.6 3,271,110 + Sama_2737 0.61 -0.8 3,271,110 + Sama_2737 0.61 -0.7 3,271,110 + Sama_2737 0.61 -0.9 3,271,111 - Sama_2737 0.61 -0.9 3,271,111 - Sama_2737 0.61 -0.5 3,271,111 - Sama_2737 0.61 +0.9 3,271,111 - Sama_2737 0.61 -1.1 3,271,111 - Sama_2737 0.61 -2.6 3,271,111 - Sama_2737 0.61 +2.0 3,271,111 - Sama_2737 0.61 -0.6 3,271,111 - Sama_2737 0.61 -0.5 3,271,111 - Sama_2737 0.61 -0.5 3,271,111 - Sama_2737 0.61 -1.9 3,271,111 - Sama_2737 0.61 +0.8 3,271,111 - Sama_2737 0.61 +0.8 3,271,144 - Sama_2737 0.63 -0.6 3,271,144 - Sama_2737 0.63 -2.5 3,271,144 - Sama_2737 0.63 -3.8 3,271,145 + Sama_2737 0.63 -2.2 3,271,145 + Sama_2737 0.63 -1.7 3,271,146 - Sama_2737 0.63 -3.4 3,271,146 - Sama_2737 0.63 +0.8 3,271,204 + Sama_2737 0.66 -0.5 3,271,204 + Sama_2737 0.66 -1.1 3,271,204 + Sama_2737 0.66 +0.4 3,271,268 + Sama_2737 0.70 -1.2 3,271,269 - Sama_2737 0.70 -0.7 3,271,272 + Sama_2737 0.70 -2.2 3,271,272 + Sama_2737 0.70 +2.1 3,271,272 + Sama_2737 0.70 -3.4 3,271,272 + Sama_2737 0.70 +2.2 3,271,272 + Sama_2737 0.70 +1.4 3,271,272 + Sama_2737 0.70 +1.8 3,271,272 + Sama_2737 0.70 -0.7 3,271,272 + Sama_2737 0.70 -1.2 3,271,273 - Sama_2737 0.70 -1.3 3,271,377 - Sama_2737 0.76 -2.6 3,271,395 - Sama_2737 0.77 -0.6 3,271,395 - Sama_2737 0.77 +1.3 3,271,456 + Sama_2737 0.81 -1.2 3,271,476 - Sama_2737 0.82 +0.8 3,271,476 - Sama_2737 0.82 +0.9 3,271,497 - Sama_2737 0.83 -3.4 3,271,574 + Sama_2737 0.88 +0.7 3,271,574 + Sama_2737 0.88 +0.6 3,271,574 + Sama_2737 0.88 -2.4 3,271,575 - Sama_2737 0.88 +0.5 3,271,575 - Sama_2737 0.88 -4.1 3,271,634 + -0.5 3,271,634 + -0.9 3,271,634 + -1.0 3,271,634 + -0.4 3,271,634 + -2.8 3,271,634 + +0.3 3,271,634 + -1.2 3,271,634 + -1.9 3,271,634 + -0.3 3,271,635 - -1.7 3,271,635 - +2.2 3,271,635 - -0.8 3,271,687 - +0.2 3,271,714 - -2.1 3,271,714 - -2.8 3,271,777 + +2.2 3,271,777 + -0.6 3,271,777 + -0.5 3,271,777 + -1.7 3,271,778 - -0.6 3,271,778 - +1.1 3,271,821 + -0.2 3,271,821 + +1.1 3,271,821 + +0.1 3,271,821 + +0.7 3,271,822 - -1.6 3,271,822 - -0.5 3,271,822 - -1.1 3,271,822 - +2.5 3,271,822 - -1.0 3,271,822 - +0.8 3,271,822 - -1.5 3,271,822 - +0.1 3,271,873 + +0.5 3,271,977 + Sama_2738 0.14 -1.1 3,271,978 - Sama_2738 0.15 +2.4 3,272,002 + Sama_2738 0.18 -1.9 3,272,003 - Sama_2738 0.18 -0.5 3,272,131 - Sama_2738 0.36 +0.3 3,272,131 - Sama_2738 0.36 -1.5 3,272,181 - Sama_2738 0.42 -2.2 3,272,182 + Sama_2738 0.43 -1.6 3,272,183 - Sama_2738 0.43 +0.9 3,272,183 - Sama_2738 0.43 -0.2 3,272,215 + Sama_2738 0.47 -1.7 3,272,271 - Sama_2738 0.55 -2.1 3,272,271 - Sama_2738 0.55 -2.6 3,272,368 + Sama_2738 0.68 -2.2 3,272,369 - Sama_2738 0.68 +1.5 3,272,410 + Sama_2738 0.74 -2.6 3,272,410 + Sama_2738 0.74 +1.9 3,272,411 - Sama_2738 0.74 -2.7 3,272,411 - Sama_2738 0.74 -0.3 3,272,629 + -0.9 3,272,630 - -0.8 3,272,630 - +0.6 3,272,630 - +1.0 3,272,641 + -1.5 3,272,643 + -2.4 3,272,643 + -2.2 3,272,643 + -1.4 3,272,643 + -2.9 3,272,644 - +1.5 3,272,644 - +0.3 3,272,644 - +0.3 3,272,644 - +1.4 3,272,644 - -1.4 3,272,696 + -2.0 3,272,697 - -0.6 3,272,714 + +1.1 3,272,714 + -1.2 3,272,715 - +1.3
Or see this region's nucleotide sequence