Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secF and secD are separated by 13 nucleotides secD and yajC are separated by 18 nucleotides yajC and tgt are separated by 26 nucleotides
Sama_2190: secF - preprotein translocase subunit SecF (RefSeq), at 2,648,150 to 2,649,097
secF
Sama_2191: secD - preprotein translocase subunit SecD (RefSeq), at 2,649,111 to 2,650,961
secD
Sama_2192: yajC - preprotein translocase subunit YajC (RefSeq), at 2,650,980 to 2,651,315
yajC
Sama_2193: tgt - queuine tRNA-ribosyltransferase (RefSeq), at 2,651,342 to 2,652,466
tgt
Position (kb)
2649
2650
2651 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2648.134 kb on - strand at 2648.143 kb on - strand at 2648.143 kb on - strand at 2648.151 kb on + strand at 2648.151 kb on + strand at 2648.152 kb on - strand at 2648.152 kb on - strand at 2648.254 kb on - strand, within secF at 2648.254 kb on - strand, within secF at 2648.266 kb on - strand, within secF at 2648.266 kb on - strand, within secF at 2648.381 kb on - strand, within secF at 2648.480 kb on - strand, within secF at 2648.480 kb on - strand, within secF at 2648.644 kb on - strand, within secF at 2649.138 kb on - strand at 2649.386 kb on - strand, within secD at 2649.545 kb on - strand, within secD at 2649.548 kb on - strand, within secD at 2649.548 kb on - strand, within secD at 2649.780 kb on - strand, within secD at 2649.780 kb on - strand, within secD at 2649.822 kb on - strand, within secD at 2649.842 kb on - strand, within secD at 2649.842 kb on - strand, within secD at 2649.842 kb on - strand, within secD at 2650.304 kb on - strand, within secD at 2650.304 kb on - strand, within secD at 2650.330 kb on - strand, within secD at 2650.476 kb on - strand, within secD at 2650.895 kb on - strand at 2651.344 kb on - strand at 2651.344 kb on - strand at 2651.352 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.383 kb on - strand at 2651.406 kb on - strand at 2651.458 kb on - strand, within tgt at 2651.477 kb on - strand, within tgt at 2651.551 kb on + strand, within tgt at 2651.554 kb on - strand, within tgt at 2651.567 kb on - strand, within tgt at 2651.670 kb on - strand, within tgt at 2651.742 kb on + strand, within tgt at 2651.839 kb on - strand, within tgt at 2651.895 kb on + strand, within tgt at 2651.896 kb on - strand, within tgt at 2651.896 kb on - strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,648,134 - +1.8 2,648,143 - -0.4 2,648,143 - -3.4 2,648,151 + +1.1 2,648,151 + -1.4 2,648,152 - +1.0 2,648,152 - +1.1 2,648,254 - secF Sama_2190 0.11 -0.4 2,648,254 - secF Sama_2190 0.11 +0.1 2,648,266 - secF Sama_2190 0.12 -0.9 2,648,266 - secF Sama_2190 0.12 +0.1 2,648,381 - secF Sama_2190 0.24 +0.1 2,648,480 - secF Sama_2190 0.35 -0.4 2,648,480 - secF Sama_2190 0.35 -0.4 2,648,644 - secF Sama_2190 0.52 +0.1 2,649,138 - -0.4 2,649,386 - secD Sama_2191 0.15 -0.4 2,649,545 - secD Sama_2191 0.23 -0.8 2,649,548 - secD Sama_2191 0.24 -0.4 2,649,548 - secD Sama_2191 0.24 +0.2 2,649,780 - secD Sama_2191 0.36 -0.8 2,649,780 - secD Sama_2191 0.36 +1.2 2,649,822 - secD Sama_2191 0.38 -1.8 2,649,842 - secD Sama_2191 0.39 +1.2 2,649,842 - secD Sama_2191 0.39 -0.8 2,649,842 - secD Sama_2191 0.39 -0.8 2,650,304 - secD Sama_2191 0.64 -2.3 2,650,304 - secD Sama_2191 0.64 -0.4 2,650,330 - secD Sama_2191 0.66 -1.7 2,650,476 - secD Sama_2191 0.74 -1.4 2,650,895 - -1.4 2,651,344 - +1.0 2,651,344 - -0.8 2,651,352 - +0.2 2,651,383 - -1.8 2,651,383 - +1.2 2,651,383 - -0.6 2,651,383 - -0.4 2,651,383 - -2.0 2,651,406 - -0.8 2,651,458 - tgt Sama_2193 0.10 -1.7 2,651,477 - tgt Sama_2193 0.12 -1.6 2,651,551 + tgt Sama_2193 0.19 +1.2 2,651,554 - tgt Sama_2193 0.19 -1.8 2,651,567 - tgt Sama_2193 0.20 -2.0 2,651,670 - tgt Sama_2193 0.29 -0.8 2,651,742 + tgt Sama_2193 0.36 +1.2 2,651,839 - tgt Sama_2193 0.44 +2.7 2,651,895 + tgt Sama_2193 0.49 -0.8 2,651,896 - tgt Sama_2193 0.49 +2.0 2,651,896 - tgt Sama_2193 0.49 -0.7
Or see this region's nucleotide sequence