Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemH and Sama_1317 are separated by 251 nucleotides Sama_1317 and Sama_1318 are separated by 207 nucleotides Sama_1318 and Sama_1319 are separated by 145 nucleotides
Sama_1316: hemH - ferrochelatase (RefSeq), at 1,599,916 to 1,600,899
hemH
Sama_1317: Sama_1317 - inosine kinase (RefSeq), at 1,601,151 to 1,602,455
_1317
Sama_1318: Sama_1318 - IS1004 transposase (RefSeq), at 1,602,663 to 1,603,109
_1318
Sama_1319: Sama_1319 - symporter for L-glutamate, L-glutamine, and L-aspartate (from data) , at 1,603,255 to 1,604,568
_1319
Position (kb)
1601
1602
1603 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1600.941 kb on - strand at 1601.044 kb on + strand at 1601.044 kb on + strand at 1601.053 kb on - strand at 1601.127 kb on + strand at 1601.150 kb on + strand at 1601.184 kb on + strand at 1601.185 kb on - strand at 1601.185 kb on - strand at 1601.253 kb on + strand at 1601.253 kb on + strand at 1601.253 kb on + strand at 1601.253 kb on + strand at 1601.253 kb on + strand at 1601.253 kb on + strand at 1601.253 kb on + strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.254 kb on - strand at 1601.256 kb on - strand at 1601.265 kb on - strand at 1601.323 kb on - strand, within Sama_1317 at 1601.399 kb on + strand, within Sama_1317 at 1601.400 kb on - strand, within Sama_1317 at 1601.432 kb on + strand, within Sama_1317 at 1601.433 kb on - strand, within Sama_1317 at 1601.446 kb on + strand, within Sama_1317 at 1601.447 kb on - strand, within Sama_1317 at 1601.454 kb on + strand, within Sama_1317 at 1601.454 kb on + strand, within Sama_1317 at 1601.454 kb on + strand, within Sama_1317 at 1601.455 kb on - strand, within Sama_1317 at 1601.455 kb on - strand, within Sama_1317 at 1601.523 kb on + strand, within Sama_1317 at 1601.523 kb on + strand, within Sama_1317 at 1601.524 kb on - strand, within Sama_1317 at 1601.529 kb on + strand, within Sama_1317 at 1601.530 kb on - strand, within Sama_1317 at 1601.535 kb on + strand, within Sama_1317 at 1601.535 kb on + strand, within Sama_1317 at 1601.536 kb on - strand, within Sama_1317 at 1601.536 kb on - strand, within Sama_1317 at 1601.595 kb on - strand, within Sama_1317 at 1601.636 kb on + strand, within Sama_1317 at 1601.685 kb on - strand, within Sama_1317 at 1601.879 kb on + strand, within Sama_1317 at 1601.900 kb on - strand, within Sama_1317 at 1602.013 kb on + strand, within Sama_1317 at 1602.014 kb on - strand, within Sama_1317 at 1602.014 kb on - strand, within Sama_1317 at 1602.021 kb on + strand, within Sama_1317 at 1602.021 kb on + strand, within Sama_1317 at 1602.022 kb on - strand, within Sama_1317 at 1602.022 kb on - strand, within Sama_1317 at 1602.023 kb on + strand, within Sama_1317 at 1602.023 kb on + strand, within Sama_1317 at 1602.024 kb on - strand, within Sama_1317 at 1602.024 kb on - strand, within Sama_1317 at 1602.033 kb on + strand, within Sama_1317 at 1602.034 kb on - strand, within Sama_1317 at 1602.129 kb on - strand, within Sama_1317 at 1602.151 kb on + strand, within Sama_1317 at 1602.151 kb on + strand, within Sama_1317 at 1602.159 kb on + strand, within Sama_1317 at 1602.159 kb on + strand, within Sama_1317 at 1602.159 kb on + strand, within Sama_1317 at 1602.159 kb on + strand, within Sama_1317 at 1602.159 kb on + strand, within Sama_1317 at 1602.159 kb on + strand, within Sama_1317 at 1602.160 kb on - strand, within Sama_1317 at 1602.167 kb on + strand, within Sama_1317 at 1602.167 kb on + strand, within Sama_1317 at 1602.167 kb on + strand, within Sama_1317 at 1602.177 kb on + strand, within Sama_1317 at 1602.177 kb on + strand, within Sama_1317 at 1602.177 kb on + strand, within Sama_1317 at 1602.178 kb on - strand, within Sama_1317 at 1602.178 kb on - strand, within Sama_1317 at 1602.179 kb on + strand, within Sama_1317 at 1602.180 kb on - strand, within Sama_1317 at 1602.279 kb on - strand, within Sama_1317 at 1602.279 kb on - strand, within Sama_1317 at 1602.296 kb on + strand, within Sama_1317 at 1602.321 kb on + strand, within Sama_1317 at 1602.321 kb on + strand, within Sama_1317 at 1602.349 kb on - strand at 1602.366 kb on + strand at 1602.367 kb on - strand at 1602.442 kb on - strand at 1602.442 kb on - strand at 1602.472 kb on + strand at 1602.472 kb on + strand at 1602.473 kb on - strand at 1602.500 kb on + strand at 1602.500 kb on + strand at 1602.500 kb on + strand at 1602.500 kb on + strand at 1602.501 kb on - strand at 1602.567 kb on - strand at 1602.567 kb on - strand at 1603.182 kb on - strand at 1603.201 kb on - strand at 1603.257 kb on - strand at 1603.374 kb on + strand at 1603.374 kb on + strand at 1603.375 kb on - strand at 1603.417 kb on - strand, within Sama_1319 at 1603.417 kb on - strand, within Sama_1319
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,600,941 - -2.8 1,601,044 + -2.1 1,601,044 + +0.6 1,601,053 - -1.1 1,601,127 + +1.8 1,601,150 + +0.3 1,601,184 + -2.7 1,601,185 - +0.6 1,601,185 - +0.2 1,601,253 + -0.3 1,601,253 + +0.9 1,601,253 + -1.4 1,601,253 + -0.7 1,601,253 + -1.4 1,601,253 + -1.1 1,601,253 + -0.4 1,601,254 - -2.2 1,601,254 - -1.4 1,601,254 - -1.7 1,601,254 - +2.3 1,601,254 - -2.4 1,601,254 - -0.7 1,601,254 - +2.7 1,601,254 - -1.1 1,601,254 - -1.4 1,601,254 - -2.2 1,601,256 - +0.6 1,601,265 - -3.0 1,601,323 - Sama_1317 0.13 -0.7 1,601,399 + Sama_1317 0.19 +0.5 1,601,400 - Sama_1317 0.19 +0.6 1,601,432 + Sama_1317 0.22 +0.2 1,601,433 - Sama_1317 0.22 +1.2 1,601,446 + Sama_1317 0.23 -0.3 1,601,447 - Sama_1317 0.23 -1.1 1,601,454 + Sama_1317 0.23 -1.4 1,601,454 + Sama_1317 0.23 -1.4 1,601,454 + Sama_1317 0.23 -2.9 1,601,455 - Sama_1317 0.23 +1.4 1,601,455 - Sama_1317 0.23 +2.2 1,601,523 + Sama_1317 0.29 -1.1 1,601,523 + Sama_1317 0.29 -2.1 1,601,524 - Sama_1317 0.29 +0.9 1,601,529 + Sama_1317 0.29 -0.1 1,601,530 - Sama_1317 0.29 -1.7 1,601,535 + Sama_1317 0.29 -1.1 1,601,535 + Sama_1317 0.29 -2.4 1,601,536 - Sama_1317 0.30 -0.1 1,601,536 - Sama_1317 0.30 -2.9 1,601,595 - Sama_1317 0.34 -2.4 1,601,636 + Sama_1317 0.37 -2.5 1,601,685 - Sama_1317 0.41 -1.7 1,601,879 + Sama_1317 0.56 -2.2 1,601,900 - Sama_1317 0.57 -1.4 1,602,013 + Sama_1317 0.66 -2.1 1,602,014 - Sama_1317 0.66 -1.8 1,602,014 - Sama_1317 0.66 -0.7 1,602,021 + Sama_1317 0.67 -1.1 1,602,021 + Sama_1317 0.67 -0.7 1,602,022 - Sama_1317 0.67 -0.7 1,602,022 - Sama_1317 0.67 -0.2 1,602,023 + Sama_1317 0.67 -0.2 1,602,023 + Sama_1317 0.67 -1.4 1,602,024 - Sama_1317 0.67 -0.7 1,602,024 - Sama_1317 0.67 -2.2 1,602,033 + Sama_1317 0.68 +0.3 1,602,034 - Sama_1317 0.68 -1.7 1,602,129 - Sama_1317 0.75 -0.2 1,602,151 + Sama_1317 0.77 -2.2 1,602,151 + Sama_1317 0.77 -0.9 1,602,159 + Sama_1317 0.77 -1.9 1,602,159 + Sama_1317 0.77 -2.4 1,602,159 + Sama_1317 0.77 -1.4 1,602,159 + Sama_1317 0.77 +3.5 1,602,159 + Sama_1317 0.77 -2.2 1,602,159 + Sama_1317 0.77 -1.7 1,602,160 - Sama_1317 0.77 -1.1 1,602,167 + Sama_1317 0.78 +0.2 1,602,167 + Sama_1317 0.78 -2.1 1,602,167 + Sama_1317 0.78 -0.3 1,602,177 + Sama_1317 0.79 -0.2 1,602,177 + Sama_1317 0.79 -1.7 1,602,177 + Sama_1317 0.79 +1.9 1,602,178 - Sama_1317 0.79 +0.5 1,602,178 - Sama_1317 0.79 +0.4 1,602,179 + Sama_1317 0.79 +0.9 1,602,180 - Sama_1317 0.79 -1.7 1,602,279 - Sama_1317 0.86 +1.0 1,602,279 - Sama_1317 0.86 -0.8 1,602,296 + Sama_1317 0.88 +0.9 1,602,321 + Sama_1317 0.90 -2.0 1,602,321 + Sama_1317 0.90 +1.2 1,602,349 - -1.4 1,602,366 + +0.6 1,602,367 - -0.7 1,602,442 - -0.1 1,602,442 - +0.7 1,602,472 + +1.6 1,602,472 + -0.2 1,602,473 - -2.7 1,602,500 + +0.2 1,602,500 + -0.7 1,602,500 + -3.1 1,602,500 + +0.6 1,602,501 - -1.7 1,602,567 - -0.9 1,602,567 - -1.2 1,603,182 - +0.8 1,603,201 - -0.1 1,603,257 - +0.2 1,603,374 + -0.4 1,603,374 + +0.1 1,603,375 - +1.2 1,603,417 - Sama_1319 0.12 -0.9 1,603,417 - Sama_1319 0.12 -1.7
Or see this region's nucleotide sequence