Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0920 and rpsT are separated by 54 nucleotides rpsT and Sama_0922 are separated by 253 nucleotides Sama_0922 and Sama_0923 are separated by 58 nucleotides
Sama_0920: Sama_0920 - transcriptional regulator HlyU (RefSeq), at 1,112,941 to 1,113,237
_0920
Sama_0921: rpsT - 30S ribosomal protein S20 (RefSeq), at 1,113,292 to 1,113,558
rpsT
Sama_0922: Sama_0922 - integral membrane protein MviN (RefSeq), at 1,113,812 to 1,115,377
_0922
Sama_0923: Sama_0923 - riboflavin biosynthesis protein RibF (RefSeq), at 1,115,436 to 1,116,371
_0923
Position (kb)
1113
1114
1115
1116 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1112.823 kb on - strand at 1112.826 kb on - strand at 1112.829 kb on + strand at 1112.829 kb on + strand at 1112.889 kb on + strand at 1112.889 kb on + strand at 1112.900 kb on + strand at 1112.900 kb on + strand at 1112.901 kb on - strand at 1112.902 kb on + strand at 1112.902 kb on + strand at 1112.902 kb on + strand at 1112.902 kb on - strand at 1112.903 kb on - strand at 1112.903 kb on - strand at 1112.903 kb on - strand at 1112.903 kb on - strand at 1112.905 kb on + strand at 1112.921 kb on - strand at 1112.923 kb on + strand at 1112.924 kb on - strand at 1112.946 kb on + strand at 1112.954 kb on + strand at 1112.954 kb on + strand at 1112.954 kb on + strand at 1112.981 kb on + strand, within Sama_0920 at 1112.991 kb on - strand, within Sama_0920 at 1113.087 kb on - strand, within Sama_0920 at 1113.087 kb on - strand, within Sama_0920 at 1113.132 kb on + strand, within Sama_0920 at 1113.132 kb on + strand, within Sama_0920 at 1113.133 kb on - strand, within Sama_0920 at 1113.168 kb on - strand, within Sama_0920 at 1113.194 kb on + strand, within Sama_0920 at 1113.219 kb on + strand at 1113.226 kb on + strand at 1113.226 kb on + strand at 1113.226 kb on + strand at 1113.226 kb on + strand at 1113.226 kb on + strand at 1113.227 kb on - strand at 1113.227 kb on - strand at 1113.227 kb on - strand at 1113.227 kb on - strand at 1115.341 kb on + strand at 1115.341 kb on + strand at 1115.342 kb on - strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.397 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.399 kb on + strand at 1115.412 kb on + strand at 1115.412 kb on + strand at 1115.416 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,112,823 - -0.1 1,112,826 - +0.1 1,112,829 + +2.3 1,112,829 + -2.1 1,112,889 + -0.5 1,112,889 + +0.9 1,112,900 + +0.4 1,112,900 + -0.9 1,112,901 - +0.9 1,112,902 + +1.4 1,112,902 + +1.3 1,112,902 + +1.1 1,112,902 - +1.6 1,112,903 - +2.0 1,112,903 - -2.5 1,112,903 - -0.4 1,112,903 - +0.9 1,112,905 + +0.2 1,112,921 - -0.1 1,112,923 + +0.9 1,112,924 - +0.7 1,112,946 + +1.2 1,112,954 + -0.3 1,112,954 + +0.6 1,112,954 + -1.3 1,112,981 + Sama_0920 0.13 -2.3 1,112,991 - Sama_0920 0.17 +1.8 1,113,087 - Sama_0920 0.49 -2.1 1,113,087 - Sama_0920 0.49 +2.8 1,113,132 + Sama_0920 0.64 +0.3 1,113,132 + Sama_0920 0.64 +1.2 1,113,133 - Sama_0920 0.65 +0.7 1,113,168 - Sama_0920 0.76 -1.3 1,113,194 + Sama_0920 0.85 -0.5 1,113,219 + +1.2 1,113,226 + -0.1 1,113,226 + +0.8 1,113,226 + +1.3 1,113,226 + +0.9 1,113,226 + -0.5 1,113,227 - +2.4 1,113,227 - -0.3 1,113,227 - +0.3 1,113,227 - -0.9 1,115,341 + +1.4 1,115,341 + +0.3 1,115,342 - -0.2 1,115,397 + +1.5 1,115,397 + -0.5 1,115,397 + +2.2 1,115,397 + -2.8 1,115,397 + +0.7 1,115,397 + +1.7 1,115,397 + +2.0 1,115,397 + -1.3 1,115,399 + +1.4 1,115,399 + +1.0 1,115,399 + -0.8 1,115,399 + -1.3 1,115,399 + -4.1 1,115,399 + +3.4 1,115,399 + -1.3 1,115,399 + -0.9 1,115,399 + +1.6 1,115,399 + -0.8 1,115,412 + -0.8 1,115,412 + -2.5 1,115,416 + +1.5
Or see this region's nucleotide sequence