Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt valS and Sama_0791 are separated by 15 nucleotides Sama_0791 and Sama_0792 are separated by 130 nucleotides Sama_0792 and Sama_0793 are separated by 119 nucleotides
Sama_0790: valS - valyl-tRNA synthetase (RefSeq), at 973,085 to 975,946
valS
Sama_0791: Sama_0791 - DNA polymerase III, chi subunit (RefSeq), at 975,962 to 976,417
_0791
Sama_0792: Sama_0792 - TetR family transcriptional regulator (RefSeq), at 976,548 to 977,186
_0792
Sama_0793: Sama_0793 - outer membrane protein RomA (RefSeq), at 977,306 to 978,382
_0793
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 975.969 kb on - strand at 976.119 kb on - strand, within Sama_0791 at 976.306 kb on + strand, within Sama_0791 at 976.306 kb on + strand, within Sama_0791 at 976.306 kb on + strand, within Sama_0791 at 976.307 kb on - strand, within Sama_0791 at 976.307 kb on - strand, within Sama_0791 at 976.308 kb on + strand, within Sama_0791 at 976.308 kb on + strand, within Sama_0791 at 976.308 kb on + strand, within Sama_0791 at 976.309 kb on - strand, within Sama_0791 at 976.309 kb on - strand, within Sama_0791 at 976.309 kb on - strand, within Sama_0791 at 976.394 kb on + strand at 976.394 kb on + strand at 976.394 kb on + strand at 976.395 kb on - strand at 976.395 kb on - strand at 976.395 kb on - strand at 976.398 kb on + strand at 976.398 kb on + strand at 976.398 kb on + strand at 976.399 kb on - strand at 976.399 kb on - strand at 976.433 kb on - strand at 976.433 kb on - strand at 976.448 kb on - strand at 976.454 kb on + strand at 976.454 kb on + strand at 976.454 kb on + strand at 976.454 kb on + strand at 976.454 kb on + strand at 976.454 kb on + strand at 976.455 kb on - strand at 976.455 kb on - strand at 976.455 kb on - strand at 976.467 kb on + strand at 976.468 kb on - strand at 976.472 kb on + strand at 976.472 kb on + strand at 976.472 kb on + strand at 976.472 kb on + strand at 976.473 kb on - strand at 976.473 kb on - strand at 976.473 kb on - strand at 976.473 kb on - strand at 976.473 kb on - strand at 976.473 kb on - strand at 976.489 kb on + strand at 976.489 kb on + strand at 976.489 kb on + strand at 976.549 kb on + strand at 976.549 kb on + strand at 976.550 kb on - strand at 976.550 kb on - strand at 976.550 kb on - strand at 976.550 kb on - strand at 976.550 kb on - strand at 976.550 kb on - strand at 976.563 kb on + strand at 976.567 kb on + strand at 976.568 kb on - strand at 976.568 kb on - strand at 976.673 kb on + strand, within Sama_0792 at 976.691 kb on + strand, within Sama_0792 at 976.691 kb on + strand, within Sama_0792 at 976.691 kb on + strand, within Sama_0792 at 976.691 kb on + strand, within Sama_0792 at 976.691 kb on + strand, within Sama_0792 at 976.691 kb on + strand, within Sama_0792 at 976.692 kb on - strand, within Sama_0792 at 976.692 kb on - strand, within Sama_0792 at 976.692 kb on - strand, within Sama_0792 at 976.692 kb on - strand, within Sama_0792 at 976.692 kb on - strand, within Sama_0792 at 976.737 kb on - strand, within Sama_0792 at 976.802 kb on + strand, within Sama_0792 at 976.802 kb on + strand, within Sama_0792 at 976.802 kb on + strand, within Sama_0792 at 976.803 kb on - strand, within Sama_0792 at 976.803 kb on - strand, within Sama_0792 at 976.803 kb on - strand, within Sama_0792 at 976.803 kb on - strand, within Sama_0792 at 976.823 kb on + strand, within Sama_0792 at 976.823 kb on + strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.824 kb on - strand, within Sama_0792 at 976.893 kb on - strand, within Sama_0792 at 976.893 kb on - strand, within Sama_0792 at 976.893 kb on - strand, within Sama_0792 at 976.893 kb on - strand, within Sama_0792 at 976.897 kb on - strand, within Sama_0792 at 976.897 kb on - strand, within Sama_0792 at 976.941 kb on + strand, within Sama_0792 at 976.942 kb on - strand, within Sama_0792 at 976.942 kb on - strand, within Sama_0792 at 976.942 kb on - strand, within Sama_0792 at 976.942 kb on - strand, within Sama_0792 at 977.021 kb on + strand, within Sama_0792 at 977.023 kb on + strand, within Sama_0792 at 977.023 kb on + strand, within Sama_0792 at 977.023 kb on + strand, within Sama_0792 at 977.023 kb on + strand, within Sama_0792 at 977.024 kb on - strand, within Sama_0792 at 977.024 kb on - strand, within Sama_0792 at 977.024 kb on - strand, within Sama_0792 at 977.024 kb on - strand, within Sama_0792 at 977.024 kb on - strand, within Sama_0792 at 977.024 kb on - strand, within Sama_0792 at 977.082 kb on + strand, within Sama_0792 at 977.121 kb on + strand, within Sama_0792 at 977.121 kb on + strand, within Sama_0792 at 977.121 kb on + strand, within Sama_0792 at 977.122 kb on - strand, within Sama_0792 at 977.190 kb on - strand at 977.237 kb on + strand at 977.277 kb on + strand at 977.302 kb on + strand at 977.302 kb on + strand at 977.302 kb on + strand at 977.302 kb on + strand at 977.303 kb on - strand at 977.303 kb on - strand at 977.303 kb on - strand at 977.303 kb on - strand at 977.353 kb on + strand at 977.356 kb on + strand at 977.357 kb on - strand at 977.369 kb on + strand at 977.369 kb on + strand at 977.370 kb on - strand at 977.370 kb on - strand at 977.370 kb on - strand at 977.370 kb on - strand at 977.411 kb on + strand at 977.411 kb on + strand at 977.411 kb on + strand at 977.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 975,969 - -1.2 976,119 - Sama_0791 0.34 -2.5 976,306 + Sama_0791 0.75 -3.0 976,306 + Sama_0791 0.75 +0.3 976,306 + Sama_0791 0.75 -0.9 976,307 - Sama_0791 0.76 +1.3 976,307 - Sama_0791 0.76 -2.4 976,308 + Sama_0791 0.76 -0.9 976,308 + Sama_0791 0.76 -1.2 976,308 + Sama_0791 0.76 +0.8 976,309 - Sama_0791 0.76 -2.9 976,309 - Sama_0791 0.76 +0.7 976,309 - Sama_0791 0.76 -2.1 976,394 + -0.9 976,394 + -0.7 976,394 + +0.3 976,395 - -2.1 976,395 - -1.2 976,395 - -3.1 976,398 + +0.9 976,398 + -2.9 976,398 + -0.6 976,399 - -0.1 976,399 - -0.8 976,433 - +0.2 976,433 - -1.7 976,448 - -2.6 976,454 + -0.4 976,454 + -1.2 976,454 + +0.7 976,454 + +0.5 976,454 + +1.4 976,454 + +1.0 976,455 - -2.4 976,455 - +0.8 976,455 - +0.3 976,467 + -1.2 976,468 - -3.4 976,472 + +1.2 976,472 + -0.4 976,472 + +0.3 976,472 + +1.3 976,473 - -0.7 976,473 - -2.6 976,473 - +0.6 976,473 - +0.9 976,473 - +0.3 976,473 - -0.1 976,489 + +0.6 976,489 + +0.1 976,489 + +0.8 976,549 + -1.7 976,549 + +1.7 976,550 - +1.4 976,550 - +1.1 976,550 - -0.4 976,550 - -2.3 976,550 - +0.6 976,550 - +1.2 976,563 + -0.9 976,567 + +2.4 976,568 - +1.9 976,568 - +1.5 976,673 + Sama_0792 0.20 +2.0 976,691 + Sama_0792 0.22 +0.1 976,691 + Sama_0792 0.22 -1.7 976,691 + Sama_0792 0.22 -0.5 976,691 + Sama_0792 0.22 +3.5 976,691 + Sama_0792 0.22 -1.5 976,691 + Sama_0792 0.22 +0.1 976,692 - Sama_0792 0.23 -2.5 976,692 - Sama_0792 0.23 +0.9 976,692 - Sama_0792 0.23 +1.4 976,692 - Sama_0792 0.23 +4.9 976,692 - Sama_0792 0.23 +1.2 976,737 - Sama_0792 0.30 -1.4 976,802 + Sama_0792 0.40 +1.7 976,802 + Sama_0792 0.40 +3.6 976,802 + Sama_0792 0.40 +4.7 976,803 - Sama_0792 0.40 +1.0 976,803 - Sama_0792 0.40 +2.4 976,803 - Sama_0792 0.40 -0.9 976,803 - Sama_0792 0.40 +0.4 976,823 + Sama_0792 0.43 -0.7 976,823 + Sama_0792 0.43 -1.5 976,824 - Sama_0792 0.43 +0.9 976,824 - Sama_0792 0.43 +3.6 976,824 - Sama_0792 0.43 +2.2 976,824 - Sama_0792 0.43 +0.3 976,824 - Sama_0792 0.43 -1.2 976,824 - Sama_0792 0.43 +3.3 976,824 - Sama_0792 0.43 +0.1 976,893 - Sama_0792 0.54 +3.1 976,893 - Sama_0792 0.54 +2.6 976,893 - Sama_0792 0.54 +2.2 976,893 - Sama_0792 0.54 +0.1 976,897 - Sama_0792 0.55 +3.3 976,897 - Sama_0792 0.55 +2.3 976,941 + Sama_0792 0.62 +1.2 976,942 - Sama_0792 0.62 +0.0 976,942 - Sama_0792 0.62 +1.0 976,942 - Sama_0792 0.62 -1.7 976,942 - Sama_0792 0.62 -0.0 977,021 + Sama_0792 0.74 +4.4 977,023 + Sama_0792 0.74 +2.5 977,023 + Sama_0792 0.74 +0.4 977,023 + Sama_0792 0.74 +1.9 977,023 + Sama_0792 0.74 +3.4 977,024 - Sama_0792 0.74 -1.2 977,024 - Sama_0792 0.74 -1.5 977,024 - Sama_0792 0.74 +0.5 977,024 - Sama_0792 0.74 +2.6 977,024 - Sama_0792 0.74 +0.5 977,024 - Sama_0792 0.74 +3.3 977,082 + Sama_0792 0.84 +0.1 977,121 + Sama_0792 0.90 +3.5 977,121 + Sama_0792 0.90 +1.3 977,121 + Sama_0792 0.90 +4.4 977,122 - Sama_0792 0.90 +2.3 977,190 - +1.9 977,237 + +0.5 977,277 + -0.7 977,302 + +1.1 977,302 + -2.1 977,302 + +0.3 977,302 + +0.4 977,303 - +0.4 977,303 - +2.2 977,303 - -1.0 977,303 - -1.7 977,353 + -0.5 977,356 + +0.6 977,357 - -0.5 977,369 + -2.0 977,369 + +3.6 977,370 - +0.8 977,370 - +0.6 977,370 - +0.1 977,370 - -1.9 977,411 + +1.4 977,411 + -1.2 977,411 + -2.2 977,412 - -2.2
Or see this region's nucleotide sequence