Experiment: MoLS4 with D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1520 and DvMF_1521 are separated by 125 nucleotides DvMF_1521 and DvMF_1522 are separated by 12 nucleotides DvMF_1522 and DvMF_1523 are separated by 16 nucleotides DvMF_1523 and DvMF_1524 are separated by 215 nucleotides
DvMF_1520: DvMF_1520 - NADH ubiquinone oxidoreductase 20 kDa subunit (RefSeq), at 1,876,326 to 1,876,799
_1520
DvMF_1521: DvMF_1521 - hypothetical protein (RefSeq), at 1,876,925 to 1,877,317
_1521
DvMF_1522: DvMF_1522 - NADH-ubiquinone oxidoreductase chain 49kDa (RefSeq), at 1,877,330 to 1,878,406
_1522
DvMF_1523: DvMF_1523 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 1,878,423 to 1,878,818
_1523
DvMF_1524: DvMF_1524 - flavin reductase domain protein FMN-binding (RefSeq), at 1,879,034 to 1,879,681
_1524
Position (kb)
1877
1878
1879 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1876.946 kb on - strand at 1876.946 kb on - strand at 1876.957 kb on + strand at 1876.987 kb on + strand, within DvMF_1521 at 1877.116 kb on + strand, within DvMF_1521 at 1877.666 kb on - strand, within DvMF_1522 at 1877.970 kb on + strand, within DvMF_1522 at 1878.079 kb on + strand, within DvMF_1522 at 1878.184 kb on - strand, within DvMF_1522 at 1878.459 kb on + strand at 1878.484 kb on - strand, within DvMF_1523 at 1878.484 kb on - strand, within DvMF_1523 at 1878.484 kb on - strand, within DvMF_1523 at 1878.589 kb on + strand, within DvMF_1523 at 1879.039 kb on - strand at 1879.118 kb on - strand, within DvMF_1524 at 1879.367 kb on + strand, within DvMF_1524 at 1879.375 kb on - strand, within DvMF_1524 at 1879.375 kb on - strand, within DvMF_1524
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.125 mg/ml remove 1,876,946 - -0.7 1,876,946 - -1.8 1,876,957 + +0.1 1,876,987 + DvMF_1521 0.16 -4.4 1,877,116 + DvMF_1521 0.49 -0.9 1,877,666 - DvMF_1522 0.31 -0.7 1,877,970 + DvMF_1522 0.59 -1.3 1,878,079 + DvMF_1522 0.70 -0.4 1,878,184 - DvMF_1522 0.79 -0.4 1,878,459 + -0.5 1,878,484 - DvMF_1523 0.15 -0.7 1,878,484 - DvMF_1523 0.15 -2.3 1,878,484 - DvMF_1523 0.15 -0.1 1,878,589 + DvMF_1523 0.42 -0.9 1,879,039 - -1.3 1,879,118 - DvMF_1524 0.13 -1.3 1,879,367 + DvMF_1524 0.51 -0.5 1,879,375 - DvMF_1524 0.53 -1.4 1,879,375 - DvMF_1524 0.53 -0.1
Or see this region's nucleotide sequence