Experiment: MoLS4 with D-Cycloserine 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0817 and DvMF_0818 are separated by 108 nucleotides DvMF_0818 and DvMF_0819 are separated by 115 nucleotides DvMF_0819 and DvMF_0820 overlap by 11 nucleotides DvMF_0820 and DvMF_0821 are separated by 138 nucleotides
DvMF_0817: DvMF_0817 - Biotin carboxylase (RefSeq), at 985,648 to 987,066
_0817
DvMF_0818: DvMF_0818 - type IV pilus assembly PilZ (RefSeq), at 987,175 to 987,705
_0818
DvMF_0819: DvMF_0819 - OmpA/MotB domain protein (RefSeq), at 987,821 to 988,555
_0819
DvMF_0820: DvMF_0820 - MotA/TolQ/ExbB proton channel (RefSeq), at 988,545 to 989,300
_0820
DvMF_0821: DvMF_0821 - purine nucleoside phosphorylase (RefSeq), at 989,439 to 990,284
_0821
Position (kb) 987
988
989 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 987.179 kb on - strand at 987.323 kb on + strand, within DvMF_0818 at 987.428 kb on + strand, within DvMF_0818 at 987.538 kb on + strand, within DvMF_0818 at 987.740 kb on - strand at 988.279 kb on - strand, within DvMF_0819 at 988.295 kb on - strand, within DvMF_0819 at 988.435 kb on + strand, within DvMF_0819 at 988.720 kb on + strand, within DvMF_0820 at 989.240 kb on + strand at 989.243 kb on - strand at 989.546 kb on - strand, within DvMF_0821
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.125 mg/ml remove 987,179 - -1.0 987,323 + DvMF_0818 0.28 -0.5 987,428 + DvMF_0818 0.48 -2.1 987,538 + DvMF_0818 0.68 -1.7 987,740 - +0.3 988,279 - DvMF_0819 0.62 -1.0 988,295 - DvMF_0819 0.64 -2.5 988,435 + DvMF_0819 0.84 -1.1 988,720 + DvMF_0820 0.23 -2.9 989,240 + -3.4 989,243 - -0.7 989,546 - DvMF_0821 0.13 -3.0
Or see this region's nucleotide sequence