Strain Fitness in Shewanella amazonensis SB2B around Sama_1983

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSama_1982 and Sama_1983 are separated by 14 nucleotidesSama_1983 and rpmF are separated by 14 nucleotidesrpmF and Sama_1985 are separated by 16 nucleotides Sama_1982: Sama_1982 - 3-oxoacyl-(acyl carrier protein) synthase III (RefSeq), at 2,410,331 to 2,411,290 _1982 Sama_1983: Sama_1983 - putative glycerol-3-phosphate acyltransferase PlsX (RefSeq), at 2,411,305 to 2,412,333 _1983 Sama_1984: rpmF - 50S ribosomal protein L32 (RefSeq), at 2,412,348 to 2,412,518 rpmF Sama_1985: Sama_1985 - hypothetical protein (RefSeq), at 2,412,535 to 2,413,059 _1985 Position (kb) 2411 2412 2413Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2410.496 kb on - strand, within Sama_1982at 2410.496 kb on - strand, within Sama_1982at 2410.535 kb on - strand, within Sama_1982at 2410.537 kb on + strand, within Sama_1982at 2410.753 kb on - strand, within Sama_1982at 2411.321 kb on - strandat 2411.321 kb on - strandat 2413.249 kb on + strandat 2413.250 kb on - strandat 2413.328 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,410,496 - Sama_1982 0.17 -0.7
2,410,496 - Sama_1982 0.17 -0.1
2,410,535 - Sama_1982 0.21 -0.1
2,410,537 + Sama_1982 0.21 -0.1
2,410,753 - Sama_1982 0.44 -0.7
2,411,321 - -4.6
2,411,321 - -4.5
2,413,249 + -0.1
2,413,250 - -2.1
2,413,328 + +0.6

Or see this region's nucleotide sequence