Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides PGA1_c09100 and PGA1_c09110 overlap by 4 nucleotides
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
PGA1_c09110: PGA1_c09110 - thiamine biosynthesis protein ThiC, at 935,485 to 937,284
_c09110
Position (kb)
934
935
936 Strain fitness (log2 ratio)
-2
-1
0
1 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand at 934.568 kb on - strand at 934.624 kb on - strand, within PGA1_c09100 at 934.636 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.710 kb on + strand, within PGA1_c09100 at 934.719 kb on - strand, within PGA1_c09100 at 934.846 kb on + strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.903 kb on + strand, within PGA1_c09100 at 934.943 kb on + strand, within PGA1_c09100 at 935.068 kb on - strand, within PGA1_c09100 at 935.081 kb on + strand, within PGA1_c09100 at 935.089 kb on - strand, within PGA1_c09100 at 935.139 kb on + strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.172 kb on + strand, within PGA1_c09100 at 935.177 kb on + strand, within PGA1_c09100 at 935.217 kb on - strand, within PGA1_c09100 at 935.228 kb on - strand, within PGA1_c09100 at 935.276 kb on - strand, within PGA1_c09100 at 935.351 kb on + strand, within PGA1_c09100 at 935.429 kb on - strand at 935.495 kb on + strand at 935.507 kb on + strand at 935.515 kb on - strand at 935.515 kb on - strand at 935.608 kb on + strand at 935.651 kb on - strand at 935.654 kb on + strand at 935.664 kb on + strand at 935.664 kb on + strand at 935.666 kb on + strand, within PGA1_c09110 at 935.682 kb on + strand, within PGA1_c09110 at 935.713 kb on + strand, within PGA1_c09110 at 935.779 kb on + strand, within PGA1_c09110 at 935.779 kb on + strand, within PGA1_c09110 at 935.786 kb on + strand, within PGA1_c09110 at 935.794 kb on - strand, within PGA1_c09110 at 935.950 kb on + strand, within PGA1_c09110 at 935.950 kb on + strand, within PGA1_c09110 at 935.964 kb on - strand, within PGA1_c09110 at 936.044 kb on + strand, within PGA1_c09110 at 936.076 kb on + strand, within PGA1_c09110 at 936.101 kb on - strand, within PGA1_c09110 at 936.107 kb on + strand, within PGA1_c09110 at 936.107 kb on + strand, within PGA1_c09110 at 936.126 kb on + strand, within PGA1_c09110 at 936.142 kb on + strand, within PGA1_c09110 at 936.181 kb on + strand, within PGA1_c09110 at 936.236 kb on - strand, within PGA1_c09110 at 936.349 kb on - strand, within PGA1_c09110 at 936.355 kb on + strand, within PGA1_c09110 at 936.380 kb on - strand, within PGA1_c09110 at 936.407 kb on + strand, within PGA1_c09110 at 936.440 kb on - strand, within PGA1_c09110 at 936.447 kb on - strand, within PGA1_c09110
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 933,518 + -0.4 933,575 + -0.2 933,580 + -0.7 933,581 - -1.1 933,583 - -1.1 933,583 - +0.8 933,583 - -0.6 933,583 - -0.2 933,603 - -1.0 933,689 + PGA1_c09070 0.15 -0.3 933,702 - PGA1_c09070 0.16 -0.3 933,731 + PGA1_c09070 0.20 -0.2 933,731 + PGA1_c09070 0.20 -1.0 933,739 - PGA1_c09070 0.21 -1.6 933,767 + PGA1_c09070 0.25 -1.1 933,862 + PGA1_c09070 0.37 -1.4 933,865 - PGA1_c09070 0.38 -0.6 933,878 + PGA1_c09070 0.39 +0.2 933,881 - PGA1_c09070 0.40 -0.3 933,901 - PGA1_c09070 0.42 -0.2 933,948 + PGA1_c09070 0.48 -0.6 933,972 + PGA1_c09070 0.51 -0.6 933,978 - PGA1_c09070 0.52 -0.3 933,980 - PGA1_c09070 0.52 +0.6 934,004 + PGA1_c09070 0.56 -0.1 934,004 + PGA1_c09070 0.56 -0.5 934,004 + PGA1_c09070 0.56 -0.3 934,004 + PGA1_c09070 0.56 -1.0 934,004 + PGA1_c09070 0.56 -0.3 934,004 + PGA1_c09070 0.56 -0.6 934,004 + PGA1_c09070 0.56 -1.1 934,004 + PGA1_c09070 0.56 -0.3 934,012 - PGA1_c09070 0.57 -2.1 934,012 - PGA1_c09070 0.57 -0.8 934,012 - PGA1_c09070 0.57 +0.1 934,012 - PGA1_c09070 0.57 -1.7 934,012 - PGA1_c09070 0.57 -0.1 934,012 - PGA1_c09070 0.57 -2.2 934,012 - PGA1_c09070 0.57 -0.4 934,012 - PGA1_c09070 0.57 -0.4 934,012 - PGA1_c09070 0.57 +0.7 934,076 + PGA1_c09070 0.65 -0.6 934,084 - PGA1_c09070 0.66 +0.1 934,103 - PGA1_c09070 0.68 -2.5 934,103 - PGA1_c09070 0.68 +0.1 934,108 + PGA1_c09070 0.69 -0.0 934,142 + PGA1_c09070 0.74 -0.1 934,142 + PGA1_c09070 0.74 +0.0 934,150 - PGA1_c09070 0.75 -0.8 934,152 + PGA1_c09070 0.75 -0.2 934,152 + PGA1_c09070 0.75 -1.6 934,162 + PGA1_c09070 0.76 -0.2 934,275 + -0.9 934,275 - -1.3 934,306 - +0.3 934,311 + -1.0 934,311 + -2.4 934,311 + -1.1 934,319 - -2.0 934,339 - -1.3 934,354 - -2.5 934,364 + -0.3 934,472 - PGA1_c09090 0.63 +0.1 934,513 - PGA1_c09090 0.83 +0.0 934,529 - -0.8 934,539 + +0.3 934,568 - +0.2 934,624 - PGA1_c09100 0.11 -1.1 934,636 - PGA1_c09100 0.13 -0.6 934,659 - PGA1_c09100 0.15 -0.7 934,659 - PGA1_c09100 0.15 -0.1 934,685 + PGA1_c09100 0.18 +0.5 934,685 + PGA1_c09100 0.18 -1.7 934,693 - PGA1_c09100 0.18 -0.5 934,693 - PGA1_c09100 0.18 +0.0 934,693 - PGA1_c09100 0.18 -2.4 934,710 + PGA1_c09100 0.20 +0.1 934,719 - PGA1_c09100 0.21 -2.4 934,846 + PGA1_c09100 0.34 -0.5 934,854 - PGA1_c09100 0.35 -0.7 934,854 - PGA1_c09100 0.35 -0.7 934,903 + PGA1_c09100 0.40 +0.4 934,943 + PGA1_c09100 0.44 -0.1 935,068 - PGA1_c09100 0.57 -0.7 935,081 + PGA1_c09100 0.58 -1.3 935,089 - PGA1_c09100 0.59 -0.9 935,139 + PGA1_c09100 0.64 +0.7 935,147 - PGA1_c09100 0.65 -0.6 935,147 - PGA1_c09100 0.65 +0.2 935,172 + PGA1_c09100 0.67 +1.1 935,177 + PGA1_c09100 0.68 -0.0 935,217 - PGA1_c09100 0.72 -0.8 935,228 - PGA1_c09100 0.73 -0.2 935,276 - PGA1_c09100 0.78 -0.3 935,351 + PGA1_c09100 0.86 -0.5 935,429 - -0.4 935,495 + -0.6 935,507 + +0.6 935,515 - -0.4 935,515 - +0.7 935,608 + -0.5 935,651 - -0.1 935,654 + +0.4 935,664 + +1.1 935,664 + -1.4 935,666 + PGA1_c09110 0.10 -0.4 935,682 + PGA1_c09110 0.11 -0.7 935,713 + PGA1_c09110 0.13 -0.5 935,779 + PGA1_c09110 0.16 -0.4 935,779 + PGA1_c09110 0.16 -1.1 935,786 + PGA1_c09110 0.17 -1.4 935,794 - PGA1_c09110 0.17 -1.3 935,950 + PGA1_c09110 0.26 -1.5 935,950 + PGA1_c09110 0.26 +0.0 935,964 - PGA1_c09110 0.27 +0.4 936,044 + PGA1_c09110 0.31 +0.2 936,076 + PGA1_c09110 0.33 -0.6 936,101 - PGA1_c09110 0.34 +0.5 936,107 + PGA1_c09110 0.35 -0.6 936,107 + PGA1_c09110 0.35 -0.3 936,126 + PGA1_c09110 0.36 +0.2 936,142 + PGA1_c09110 0.36 -0.6 936,181 + PGA1_c09110 0.39 -0.1 936,236 - PGA1_c09110 0.42 -0.3 936,349 - PGA1_c09110 0.48 -0.4 936,355 + PGA1_c09110 0.48 -0.2 936,380 - PGA1_c09110 0.50 -0.3 936,407 + PGA1_c09110 0.51 -0.7 936,440 - PGA1_c09110 0.53 -1.0 936,447 - PGA1_c09110 0.53 -0.4
Or see this region's nucleotide sequence