Experiment: m.b. A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00060 and PGA1_262p00070 overlap by 17 nucleotides PGA1_262p00070 and PGA1_262p00080 are separated by 28 nucleotides PGA1_262p00080 and PGA1_262p00090 are separated by 7 nucleotides
PGA1_262p00060: PGA1_262p00060 - putative glycosyl transferase, at 6,672 to 7,988
_262p00060
PGA1_262p00070: PGA1_262p00070 - Lipid A core - O-antigen ligase and related enzymes, at 7,972 to 9,258
_262p00070
PGA1_262p00080: PGA1_262p00080 - hypothetical protein, at 9,287 to 10,054
_262p00080
PGA1_262p00090: PGA1_262p00090 - putative exopolysaccharide biosynthesis transport protein, at 10,062 to 12,173
_262p00090
Position (kb)
7
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1 at 6.972 kb on - strand, within PGA1_262p00060 at 6.979 kb on + strand, within PGA1_262p00060 at 6.993 kb on - strand, within PGA1_262p00060 at 7.096 kb on + strand, within PGA1_262p00060 at 7.263 kb on - strand, within PGA1_262p00060 at 7.297 kb on - strand, within PGA1_262p00060 at 7.772 kb on + strand, within PGA1_262p00060 at 7.813 kb on + strand, within PGA1_262p00060 at 7.909 kb on - strand at 7.932 kb on + strand at 7.973 kb on - strand at 7.990 kb on - strand at 8.050 kb on - strand at 8.166 kb on - strand, within PGA1_262p00070 at 8.231 kb on + strand, within PGA1_262p00070 at 8.262 kb on + strand, within PGA1_262p00070 at 8.287 kb on - strand, within PGA1_262p00070 at 8.394 kb on + strand, within PGA1_262p00070 at 8.408 kb on + strand, within PGA1_262p00070 at 8.481 kb on - strand, within PGA1_262p00070 at 8.481 kb on - strand, within PGA1_262p00070 at 8.516 kb on - strand, within PGA1_262p00070 at 8.895 kb on + strand, within PGA1_262p00070 at 8.914 kb on + strand, within PGA1_262p00070 at 8.922 kb on - strand, within PGA1_262p00070 at 8.926 kb on + strand, within PGA1_262p00070 at 8.981 kb on + strand, within PGA1_262p00070 at 8.984 kb on + strand, within PGA1_262p00070 at 9.007 kb on + strand, within PGA1_262p00070 at 9.037 kb on - strand, within PGA1_262p00070 at 9.079 kb on - strand, within PGA1_262p00070 at 9.103 kb on - strand, within PGA1_262p00070 at 9.252 kb on + strand at 9.376 kb on - strand, within PGA1_262p00080 at 9.472 kb on - strand, within PGA1_262p00080 at 9.540 kb on - strand, within PGA1_262p00080 at 9.777 kb on - strand, within PGA1_262p00080 at 9.779 kb on + strand, within PGA1_262p00080 at 9.779 kb on + strand, within PGA1_262p00080 at 9.855 kb on - strand, within PGA1_262p00080 at 9.965 kb on - strand, within PGA1_262p00080 at 9.983 kb on + strand at 10.051 kb on + strand at 10.065 kb on + strand at 10.123 kb on + strand at 10.131 kb on - strand at 10.213 kb on + strand at 10.221 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. A22 0.02 mg/ml remove 6,972 - PGA1_262p00060 0.23 -0.6 6,979 + PGA1_262p00060 0.23 -2.6 6,993 - PGA1_262p00060 0.24 -0.2 7,096 + PGA1_262p00060 0.32 -1.4 7,263 - PGA1_262p00060 0.45 -1.0 7,297 - PGA1_262p00060 0.47 -0.1 7,772 + PGA1_262p00060 0.84 -0.3 7,813 + PGA1_262p00060 0.87 +0.4 7,909 - -1.4 7,932 + -0.1 7,973 - +0.8 7,990 - +0.3 8,050 - -0.5 8,166 - PGA1_262p00070 0.15 -0.6 8,231 + PGA1_262p00070 0.20 +0.7 8,262 + PGA1_262p00070 0.23 +0.6 8,287 - PGA1_262p00070 0.24 +0.2 8,394 + PGA1_262p00070 0.33 +1.3 8,408 + PGA1_262p00070 0.34 +1.1 8,481 - PGA1_262p00070 0.40 -0.2 8,481 - PGA1_262p00070 0.40 +0.0 8,516 - PGA1_262p00070 0.42 +0.0 8,895 + PGA1_262p00070 0.72 +0.0 8,914 + PGA1_262p00070 0.73 -0.1 8,922 - PGA1_262p00070 0.74 +0.4 8,926 + PGA1_262p00070 0.74 +0.0 8,981 + PGA1_262p00070 0.78 +0.4 8,984 + PGA1_262p00070 0.79 +0.6 9,007 + PGA1_262p00070 0.80 -0.7 9,037 - PGA1_262p00070 0.83 +0.2 9,079 - PGA1_262p00070 0.86 +0.2 9,103 - PGA1_262p00070 0.88 +0.6 9,252 + -0.6 9,376 - PGA1_262p00080 0.12 -0.4 9,472 - PGA1_262p00080 0.24 +0.2 9,540 - PGA1_262p00080 0.33 +1.6 9,777 - PGA1_262p00080 0.64 +0.5 9,779 + PGA1_262p00080 0.64 -2.6 9,779 + PGA1_262p00080 0.64 +0.1 9,855 - PGA1_262p00080 0.74 -0.5 9,965 - PGA1_262p00080 0.88 -0.1 9,983 + -0.1 10,051 + +0.9 10,065 + +0.2 10,123 + +0.9 10,131 - -0.1 10,213 + +0.3 10,221 - +0.6
Or see this region's nucleotide sequence