Experiment: MoLS4 with D-Cycloserine 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2041 and DvMF_2042 overlap by 4 nucleotides DvMF_2042 and DvMF_2043 are separated by 104 nucleotides DvMF_2043 and DvMF_2044 are separated by 345 nucleotides
DvMF_2041: DvMF_2041 - protein of unknown function DUF116 (RefSeq), at 2,531,921 to 2,532,985
_2041
DvMF_2042: DvMF_2042 - NusB/RsmB/TIM44 (RefSeq), at 2,532,982 to 2,534,574
_2042
DvMF_2043: DvMF_2043 - hypothetical protein (RefSeq), at 2,534,679 to 2,535,083
_2043
DvMF_2044: DvMF_2044 - PfkB domain protein (RefSeq), at 2,535,429 to 2,536,454
_2044
Position (kb)
2532
2533
2534
2535 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2532.068 kb on + strand, within DvMF_2041 at 2532.156 kb on + strand, within DvMF_2041 at 2532.184 kb on + strand, within DvMF_2041 at 2532.347 kb on - strand, within DvMF_2041 at 2532.494 kb on - strand, within DvMF_2041 at 2532.657 kb on + strand, within DvMF_2041 at 2532.786 kb on + strand, within DvMF_2041 at 2532.997 kb on + strand at 2533.005 kb on - strand at 2533.027 kb on + strand at 2533.059 kb on + strand at 2533.059 kb on + strand at 2533.086 kb on - strand at 2533.094 kb on - strand at 2533.096 kb on + strand at 2533.113 kb on + strand at 2533.214 kb on + strand, within DvMF_2042 at 2533.223 kb on + strand, within DvMF_2042 at 2533.224 kb on - strand, within DvMF_2042 at 2533.437 kb on + strand, within DvMF_2042 at 2533.437 kb on + strand, within DvMF_2042 at 2533.445 kb on - strand, within DvMF_2042 at 2533.546 kb on + strand, within DvMF_2042 at 2533.613 kb on + strand, within DvMF_2042 at 2533.684 kb on + strand, within DvMF_2042 at 2533.765 kb on + strand, within DvMF_2042 at 2533.828 kb on + strand, within DvMF_2042 at 2533.843 kb on + strand, within DvMF_2042 at 2533.860 kb on + strand, within DvMF_2042 at 2533.879 kb on + strand, within DvMF_2042 at 2533.951 kb on + strand, within DvMF_2042 at 2534.023 kb on - strand, within DvMF_2042 at 2534.048 kb on + strand, within DvMF_2042 at 2534.103 kb on + strand, within DvMF_2042 at 2534.147 kb on - strand, within DvMF_2042 at 2534.158 kb on + strand, within DvMF_2042 at 2534.191 kb on + strand, within DvMF_2042 at 2534.253 kb on - strand, within DvMF_2042 at 2534.264 kb on + strand, within DvMF_2042 at 2534.264 kb on + strand, within DvMF_2042 at 2534.272 kb on - strand, within DvMF_2042 at 2534.272 kb on - strand, within DvMF_2042 at 2534.272 kb on - strand, within DvMF_2042 at 2534.272 kb on - strand, within DvMF_2042 at 2534.315 kb on - strand, within DvMF_2042 at 2534.368 kb on + strand, within DvMF_2042 at 2534.382 kb on + strand, within DvMF_2042 at 2534.390 kb on - strand, within DvMF_2042 at 2534.390 kb on - strand, within DvMF_2042 at 2534.401 kb on + strand, within DvMF_2042 at 2534.403 kb on - strand, within DvMF_2042 at 2534.452 kb on + strand at 2534.460 kb on - strand at 2534.460 kb on - strand at 2534.460 kb on - strand at 2534.469 kb on - strand at 2534.562 kb on + strand at 2534.569 kb on + strand at 2534.582 kb on + strand at 2534.677 kb on + strand at 2534.753 kb on - strand, within DvMF_2043 at 2534.781 kb on + strand, within DvMF_2043 at 2534.879 kb on - strand, within DvMF_2043 at 2534.879 kb on - strand, within DvMF_2043 at 2534.899 kb on + strand, within DvMF_2043 at 2534.939 kb on - strand, within DvMF_2043 at 2534.969 kb on + strand, within DvMF_2043 at 2534.969 kb on + strand, within DvMF_2043 at 2534.974 kb on + strand, within DvMF_2043 at 2534.988 kb on - strand, within DvMF_2043 at 2535.054 kb on - strand at 2535.054 kb on - strand at 2535.068 kb on + strand at 2535.076 kb on - strand at 2535.076 kb on - strand at 2535.076 kb on - strand at 2535.207 kb on - strand at 2535.253 kb on + strand at 2535.261 kb on - strand at 2535.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.25 mg/ml remove 2,532,068 + DvMF_2041 0.14 -2.2 2,532,156 + DvMF_2041 0.22 -3.1 2,532,184 + DvMF_2041 0.25 -1.3 2,532,347 - DvMF_2041 0.40 -1.5 2,532,494 - DvMF_2041 0.54 -2.7 2,532,657 + DvMF_2041 0.69 -1.6 2,532,786 + DvMF_2041 0.81 -0.6 2,532,997 + -1.0 2,533,005 - -1.6 2,533,027 + +1.2 2,533,059 + -0.9 2,533,059 + -1.5 2,533,086 - -0.6 2,533,094 - -0.7 2,533,096 + +0.0 2,533,113 + -1.7 2,533,214 + DvMF_2042 0.15 -1.0 2,533,223 + DvMF_2042 0.15 -0.4 2,533,224 - DvMF_2042 0.15 -0.3 2,533,437 + DvMF_2042 0.29 -0.4 2,533,437 + DvMF_2042 0.29 -0.6 2,533,445 - DvMF_2042 0.29 -0.8 2,533,546 + DvMF_2042 0.35 -0.7 2,533,613 + DvMF_2042 0.40 -2.2 2,533,684 + DvMF_2042 0.44 -0.5 2,533,765 + DvMF_2042 0.49 -0.1 2,533,828 + DvMF_2042 0.53 -0.5 2,533,843 + DvMF_2042 0.54 -0.7 2,533,860 + DvMF_2042 0.55 -0.3 2,533,879 + DvMF_2042 0.56 -0.1 2,533,951 + DvMF_2042 0.61 -0.7 2,534,023 - DvMF_2042 0.65 -0.0 2,534,048 + DvMF_2042 0.67 -0.8 2,534,103 + DvMF_2042 0.70 -0.5 2,534,147 - DvMF_2042 0.73 -0.3 2,534,158 + DvMF_2042 0.74 -0.8 2,534,191 + DvMF_2042 0.76 -0.5 2,534,253 - DvMF_2042 0.80 -2.2 2,534,264 + DvMF_2042 0.80 -0.5 2,534,264 + DvMF_2042 0.80 +0.4 2,534,272 - DvMF_2042 0.81 +0.1 2,534,272 - DvMF_2042 0.81 -0.9 2,534,272 - DvMF_2042 0.81 -0.8 2,534,272 - DvMF_2042 0.81 +0.5 2,534,315 - DvMF_2042 0.84 -0.7 2,534,368 + DvMF_2042 0.87 +0.1 2,534,382 + DvMF_2042 0.88 -1.0 2,534,390 - DvMF_2042 0.88 -0.3 2,534,390 - DvMF_2042 0.88 -0.3 2,534,401 + DvMF_2042 0.89 -1.1 2,534,403 - DvMF_2042 0.89 -0.7 2,534,452 + -0.8 2,534,460 - -0.9 2,534,460 - -0.4 2,534,460 - -0.1 2,534,469 - -0.4 2,534,562 + -0.8 2,534,569 + +0.0 2,534,582 + -1.1 2,534,677 + -1.2 2,534,753 - DvMF_2043 0.18 -0.3 2,534,781 + DvMF_2043 0.25 -1.7 2,534,879 - DvMF_2043 0.49 -0.4 2,534,879 - DvMF_2043 0.49 -0.5 2,534,899 + DvMF_2043 0.54 -1.0 2,534,939 - DvMF_2043 0.64 -0.6 2,534,969 + DvMF_2043 0.72 -0.1 2,534,969 + DvMF_2043 0.72 +0.1 2,534,974 + DvMF_2043 0.73 -1.1 2,534,988 - DvMF_2043 0.76 -0.6 2,535,054 - -0.6 2,535,054 - -0.4 2,535,068 + -0.4 2,535,076 - -0.5 2,535,076 - -0.4 2,535,076 - -0.3 2,535,207 - -1.7 2,535,253 + -0.3 2,535,261 - -0.2 2,535,396 - -0.3
Or see this region's nucleotide sequence