Experiment: MoLS4 with D-Cycloserine 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0450 and DvMF_0451 are separated by 83 nucleotides DvMF_0451 and DvMF_0452 overlap by 4 nucleotides DvMF_0452 and DvMF_0453 overlap by 4 nucleotides
DvMF_0450: DvMF_0450 - 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8) <small>(from data)</small>, at 527,519 to 528,298
_0450
DvMF_0451: DvMF_0451 - DNA ligase, NAD-dependent (RefSeq), at 528,382 to 530,808
_0451
DvMF_0452: DvMF_0452 - hypothetical protein (RefSeq), at 530,805 to 531,647
_0452
DvMF_0453: DvMF_0453 - TrkA-N domain protein (RefSeq), at 531,644 to 532,684
_0453
Position (kb) 528
529
530
531 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 527.653 kb on + strand, within DvMF_0450 at 527.781 kb on - strand, within DvMF_0450 at 527.913 kb on + strand, within DvMF_0450 at 528.225 kb on - strand at 528.549 kb on + strand at 528.632 kb on + strand, within DvMF_0451 at 530.818 kb on - strand at 530.851 kb on - strand at 530.915 kb on + strand, within DvMF_0452 at 531.074 kb on + strand, within DvMF_0452 at 531.203 kb on - strand, within DvMF_0452 at 531.445 kb on + strand, within DvMF_0452 at 531.458 kb on - strand, within DvMF_0452 at 531.732 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.25 mg/ml remove 527,653 + DvMF_0450 0.17 -0.4 527,781 - DvMF_0450 0.34 -2.0 527,913 + DvMF_0450 0.51 -3.9 528,225 - -4.5 528,549 + -0.6 528,632 + DvMF_0451 0.10 -3.8 530,818 - -0.4 530,851 - -2.1 530,915 + DvMF_0452 0.13 -0.9 531,074 + DvMF_0452 0.32 -1.1 531,203 - DvMF_0452 0.47 -0.5 531,445 + DvMF_0452 0.76 -1.0 531,458 - DvMF_0452 0.77 -0.2 531,732 + +1.0
Or see this region's nucleotide sequence