Experiment: MoLS4 with D-Cycloserine 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0394 and DvMF_0395 are separated by 138 nucleotides DvMF_0395 and DvMF_0396 overlap by 8 nucleotides DvMF_0396 and DvMF_0397 overlap by 8 nucleotides
DvMF_0394: DvMF_0394 - protein of unknown function DUF1653 (RefSeq), at 465,303 to 465,671
_0394
DvMF_0395: DvMF_0395 - protein of unknown function DUF162 (RefSeq), at 465,810 to 466,466
_0395
DvMF_0396: DvMF_0396 - iron-sulfur cluster binding protein (RefSeq), at 466,459 to 467,886
_0396
DvMF_0397: DvMF_0397 - protein of unknown function DUF224 cysteine-rich region domain protein (RefSeq), at 467,879 to 468,640
_0397
Position (kb) 466
467
468 Strain fitness (log2 ratio) -2
-1
0
1 at 465.467 kb on + strand, within DvMF_0394 at 465.475 kb on - strand, within DvMF_0394 at 465.485 kb on + strand, within DvMF_0394 at 465.493 kb on - strand, within DvMF_0394 at 465.844 kb on - strand at 465.883 kb on + strand, within DvMF_0395 at 465.898 kb on + strand, within DvMF_0395 at 466.227 kb on + strand, within DvMF_0395 at 466.456 kb on + strand at 466.464 kb on - strand at 466.545 kb on - strand at 466.567 kb on - strand at 466.599 kb on + strand at 466.688 kb on + strand, within DvMF_0396 at 466.909 kb on + strand, within DvMF_0396 at 467.170 kb on + strand, within DvMF_0396 at 467.356 kb on + strand, within DvMF_0396 at 467.496 kb on + strand, within DvMF_0396 at 467.596 kb on + strand, within DvMF_0396 at 467.668 kb on - strand, within DvMF_0396 at 467.843 kb on - strand at 467.881 kb on + strand at 468.414 kb on + strand, within DvMF_0397 at 468.519 kb on - strand, within DvMF_0397 at 468.530 kb on + strand, within DvMF_0397 at 468.593 kb on - strand at 468.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with D-Cycloserine 0.25 mg/ml remove 465,467 + DvMF_0394 0.44 -0.5 465,475 - DvMF_0394 0.47 -0.9 465,485 + DvMF_0394 0.49 -0.3 465,493 - DvMF_0394 0.51 +0.0 465,844 - -0.3 465,883 + DvMF_0395 0.11 -0.4 465,898 + DvMF_0395 0.13 -1.0 466,227 + DvMF_0395 0.63 -1.8 466,456 + -1.2 466,464 - -0.3 466,545 - +0.7 466,567 - -0.1 466,599 + +0.1 466,688 + DvMF_0396 0.16 -0.8 466,909 + DvMF_0396 0.32 -1.0 467,170 + DvMF_0396 0.50 -0.6 467,356 + DvMF_0396 0.63 -0.8 467,496 + DvMF_0396 0.73 +0.3 467,596 + DvMF_0396 0.80 -1.0 467,668 - DvMF_0396 0.85 -0.3 467,843 - -0.3 467,881 + +0.1 468,414 + DvMF_0397 0.70 -2.0 468,519 - DvMF_0397 0.84 -0.6 468,530 + DvMF_0397 0.85 -0.3 468,593 - -1.0 468,803 - -0.8
Or see this region's nucleotide sequence