Experiment: BG11 with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt avtA and rimM are separated by 3 nucleotides rimM and Synpcc7942_2260 are separated by 30 nucleotides Synpcc7942_2260 and Synpcc7942_2261 are separated by 243 nucleotides Synpcc7942_2261 and Synpcc7942_2262 overlap by 4 nucleotides
Synpcc7942_2258: avtA - valine--pyruvate transaminase, at 2,327,013 to 2,328,296
avtA
Synpcc7942_2259: rimM - 16S rRNA-processing protein, at 2,328,300 to 2,328,848
rimM
Synpcc7942_2260: Synpcc7942_2260 - hypothetical protein, at 2,328,879 to 2,329,205
_2260
Synpcc7942_2261: Synpcc7942_2261 - periplasmic divalent cation tolerance protein, at 2,329,449 to 2,329,790
_2261
Synpcc7942_2262: Synpcc7942_2262 - hypothetical protein, at 2,329,787 to 2,331,196
_2262
Position (kb)
2328
2329
2330 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2327.911 kb on + strand, within avtA at 2327.911 kb on + strand, within avtA at 2327.919 kb on - strand, within avtA at 2327.919 kb on - strand, within avtA at 2327.993 kb on - strand, within avtA at 2328.012 kb on + strand, within avtA at 2328.019 kb on - strand, within avtA at 2328.020 kb on - strand, within avtA at 2328.059 kb on + strand, within avtA at 2328.059 kb on + strand, within avtA at 2328.064 kb on + strand, within avtA at 2328.064 kb on + strand, within avtA at 2328.067 kb on - strand, within avtA at 2328.067 kb on - strand, within avtA at 2328.069 kb on + strand, within avtA at 2328.072 kb on - strand, within avtA at 2328.132 kb on - strand at 2328.148 kb on - strand, within avtA at 2328.151 kb on + strand, within avtA at 2328.167 kb on + strand, within avtA at 2328.168 kb on + strand at 2328.250 kb on + strand at 2328.256 kb on + strand at 2328.256 kb on - strand at 2328.258 kb on + strand at 2328.258 kb on + strand at 2328.266 kb on - strand at 2328.266 kb on - strand at 2328.266 kb on - strand at 2328.266 kb on - strand at 2328.274 kb on - strand at 2328.343 kb on - strand at 2328.361 kb on + strand, within rimM at 2328.424 kb on + strand, within rimM at 2328.688 kb on - strand, within rimM at 2328.761 kb on - strand, within rimM at 2328.826 kb on + strand at 2328.834 kb on - strand at 2328.855 kb on + strand at 2328.914 kb on - strand, within Synpcc7942_2260 at 2328.930 kb on + strand, within Synpcc7942_2260 at 2328.930 kb on + strand, within Synpcc7942_2260 at 2328.938 kb on - strand, within Synpcc7942_2260 at 2328.938 kb on - strand, within Synpcc7942_2260 at 2328.989 kb on + strand, within Synpcc7942_2260 at 2328.989 kb on + strand, within Synpcc7942_2260 at 2328.989 kb on + strand, within Synpcc7942_2260 at 2329.022 kb on + strand, within Synpcc7942_2260 at 2329.045 kb on - strand, within Synpcc7942_2260 at 2329.079 kb on - strand, within Synpcc7942_2260 at 2329.092 kb on + strand, within Synpcc7942_2260 at 2329.092 kb on + strand, within Synpcc7942_2260 at 2329.092 kb on + strand, within Synpcc7942_2260 at 2329.100 kb on - strand, within Synpcc7942_2260 at 2329.170 kb on + strand, within Synpcc7942_2260 at 2329.178 kb on - strand at 2329.227 kb on + strand at 2329.235 kb on - strand at 2329.242 kb on + strand at 2329.259 kb on + strand at 2329.345 kb on + strand at 2329.349 kb on - strand at 2329.369 kb on + strand at 2329.374 kb on - strand at 2329.374 kb on - strand at 2329.419 kb on - strand at 2329.419 kb on - strand at 2329.432 kb on - strand at 2329.439 kb on + strand at 2329.453 kb on - strand at 2329.455 kb on - strand at 2329.457 kb on + strand at 2329.457 kb on + strand at 2329.481 kb on + strand at 2329.481 kb on + strand at 2329.487 kb on - strand, within Synpcc7942_2261 at 2329.489 kb on - strand, within Synpcc7942_2261 at 2329.508 kb on + strand, within Synpcc7942_2261 at 2329.523 kb on - strand, within Synpcc7942_2261 at 2329.527 kb on + strand, within Synpcc7942_2261 at 2329.528 kb on + strand, within Synpcc7942_2261 at 2329.535 kb on - strand, within Synpcc7942_2261 at 2329.536 kb on - strand, within Synpcc7942_2261 at 2329.572 kb on - strand, within Synpcc7942_2261 at 2329.585 kb on + strand, within Synpcc7942_2261 at 2329.594 kb on - strand, within Synpcc7942_2261 at 2329.612 kb on - strand, within Synpcc7942_2261 at 2329.652 kb on - strand, within Synpcc7942_2261 at 2329.652 kb on - strand, within Synpcc7942_2261 at 2329.655 kb on + strand, within Synpcc7942_2261 at 2329.660 kb on + strand at 2329.663 kb on - strand, within Synpcc7942_2261 at 2329.735 kb on + strand, within Synpcc7942_2261 at 2329.735 kb on + strand at 2329.735 kb on + strand, within Synpcc7942_2261 at 2329.743 kb on - strand, within Synpcc7942_2261 at 2329.752 kb on + strand, within Synpcc7942_2261 at 2329.774 kb on + strand at 2329.781 kb on + strand at 2329.784 kb on - strand at 2329.786 kb on + strand at 2329.794 kb on - strand at 2329.794 kb on - strand at 2329.797 kb on + strand at 2329.797 kb on + strand at 2329.805 kb on - strand at 2329.805 kb on - strand at 2329.821 kb on + strand at 2329.834 kb on + strand at 2329.835 kb on + strand at 2329.846 kb on + strand at 2329.846 kb on + strand at 2329.846 kb on + strand at 2329.851 kb on + strand at 2329.854 kb on - strand at 2329.854 kb on - strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.861 kb on + strand at 2329.869 kb on - strand at 2329.869 kb on - strand at 2329.869 kb on - strand at 2329.869 kb on - strand at 2329.869 kb on - strand at 2329.869 kb on - strand at 2329.869 kb on - strand at 2329.927 kb on - strand at 2329.957 kb on - strand, within Synpcc7942_2262 at 2329.959 kb on + strand, within Synpcc7942_2262 at 2329.970 kb on + strand, within Synpcc7942_2262 at 2329.973 kb on - strand, within Synpcc7942_2262 at 2329.978 kb on + strand, within Synpcc7942_2262 at 2330.011 kb on + strand at 2330.019 kb on - strand, within Synpcc7942_2262 at 2330.019 kb on - strand, within Synpcc7942_2262 at 2330.043 kb on - strand, within Synpcc7942_2262 at 2330.054 kb on - strand, within Synpcc7942_2262 at 2330.062 kb on - strand, within Synpcc7942_2262 at 2330.092 kb on + strand, within Synpcc7942_2262 at 2330.099 kb on + strand, within Synpcc7942_2262 at 2330.146 kb on + strand, within Synpcc7942_2262 at 2330.148 kb on - strand, within Synpcc7942_2262 at 2330.154 kb on - strand, within Synpcc7942_2262 at 2330.179 kb on - strand at 2330.204 kb on - strand, within Synpcc7942_2262
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrate 200 mM remove 2,327,911 + avtA Synpcc7942_2258 0.70 +0.6 2,327,911 + avtA Synpcc7942_2258 0.70 -0.2 2,327,919 - avtA Synpcc7942_2258 0.71 -1.0 2,327,919 - avtA Synpcc7942_2258 0.71 +0.2 2,327,993 - avtA Synpcc7942_2258 0.76 +0.8 2,328,012 + avtA Synpcc7942_2258 0.78 +0.6 2,328,019 - avtA Synpcc7942_2258 0.78 -2.3 2,328,020 - avtA Synpcc7942_2258 0.78 +0.3 2,328,059 + avtA Synpcc7942_2258 0.81 +0.1 2,328,059 + avtA Synpcc7942_2258 0.81 +0.3 2,328,064 + avtA Synpcc7942_2258 0.82 +0.3 2,328,064 + avtA Synpcc7942_2258 0.82 +1.4 2,328,067 - avtA Synpcc7942_2258 0.82 +1.0 2,328,067 - avtA Synpcc7942_2258 0.82 +0.6 2,328,069 + avtA Synpcc7942_2258 0.82 +0.4 2,328,072 - avtA Synpcc7942_2258 0.82 +0.3 2,328,132 - -0.2 2,328,148 - avtA Synpcc7942_2258 0.88 +0.3 2,328,151 + avtA Synpcc7942_2258 0.89 -0.2 2,328,167 + avtA Synpcc7942_2258 0.90 +0.6 2,328,168 + +0.7 2,328,250 + +1.6 2,328,256 + -0.1 2,328,256 - +0.8 2,328,258 + +1.2 2,328,258 + +0.1 2,328,266 - +0.4 2,328,266 - -1.9 2,328,266 - +0.5 2,328,266 - +1.9 2,328,274 - +1.0 2,328,343 - -1.6 2,328,361 + rimM Synpcc7942_2259 0.11 -3.2 2,328,424 + rimM Synpcc7942_2259 0.23 -1.2 2,328,688 - rimM Synpcc7942_2259 0.71 -1.7 2,328,761 - rimM Synpcc7942_2259 0.84 -2.9 2,328,826 + -3.1 2,328,834 - -4.3 2,328,855 + +0.5 2,328,914 - Synpcc7942_2260 0.11 +1.5 2,328,930 + Synpcc7942_2260 0.16 -0.5 2,328,930 + Synpcc7942_2260 0.16 -0.9 2,328,938 - Synpcc7942_2260 0.18 +2.2 2,328,938 - Synpcc7942_2260 0.18 -0.7 2,328,989 + Synpcc7942_2260 0.34 +0.9 2,328,989 + Synpcc7942_2260 0.34 +0.8 2,328,989 + Synpcc7942_2260 0.34 +2.9 2,329,022 + Synpcc7942_2260 0.44 +1.1 2,329,045 - Synpcc7942_2260 0.51 +0.1 2,329,079 - Synpcc7942_2260 0.61 +0.3 2,329,092 + Synpcc7942_2260 0.65 -0.6 2,329,092 + Synpcc7942_2260 0.65 +2.1 2,329,092 + Synpcc7942_2260 0.65 -1.0 2,329,100 - Synpcc7942_2260 0.68 +0.6 2,329,170 + Synpcc7942_2260 0.89 +1.4 2,329,178 - +0.1 2,329,227 + -0.8 2,329,235 - -1.1 2,329,242 + -0.6 2,329,259 + +0.4 2,329,345 + -1.0 2,329,349 - +1.6 2,329,369 + -0.1 2,329,374 - -0.4 2,329,374 - +0.7 2,329,419 - +1.0 2,329,419 - +0.6 2,329,432 - -1.0 2,329,439 + -0.7 2,329,453 - +0.6 2,329,455 - +0.4 2,329,457 + +0.9 2,329,457 + +0.7 2,329,481 + -0.3 2,329,481 + +1.2 2,329,487 - Synpcc7942_2261 0.11 +1.7 2,329,489 - Synpcc7942_2261 0.12 +0.2 2,329,508 + Synpcc7942_2261 0.17 +0.2 2,329,523 - Synpcc7942_2261 0.22 +0.1 2,329,527 + Synpcc7942_2261 0.23 +0.3 2,329,528 + Synpcc7942_2261 0.23 +0.2 2,329,535 - Synpcc7942_2261 0.25 -1.8 2,329,536 - Synpcc7942_2261 0.25 -2.8 2,329,572 - Synpcc7942_2261 0.36 +0.2 2,329,585 + Synpcc7942_2261 0.40 +0.2 2,329,594 - Synpcc7942_2261 0.42 -0.5 2,329,612 - Synpcc7942_2261 0.48 +0.1 2,329,652 - Synpcc7942_2261 0.59 +1.8 2,329,652 - Synpcc7942_2261 0.59 -1.8 2,329,655 + Synpcc7942_2261 0.60 -0.4 2,329,660 + +1.2 2,329,663 - Synpcc7942_2261 0.63 -1.2 2,329,735 + Synpcc7942_2261 0.84 +1.0 2,329,735 + +0.1 2,329,735 + Synpcc7942_2261 0.84 +0.1 2,329,743 - Synpcc7942_2261 0.86 -1.0 2,329,752 + Synpcc7942_2261 0.89 +0.2 2,329,774 + -1.4 2,329,781 + +0.7 2,329,784 - +0.2 2,329,786 + +0.1 2,329,794 - +1.1 2,329,794 - -0.9 2,329,797 + +0.7 2,329,797 + +0.4 2,329,805 - -0.4 2,329,805 - +1.3 2,329,821 + -0.4 2,329,834 + +0.6 2,329,835 + -0.3 2,329,846 + +0.8 2,329,846 + -0.0 2,329,846 + +1.6 2,329,851 + +0.3 2,329,854 - -0.0 2,329,854 - +1.4 2,329,861 + +2.7 2,329,861 + +1.0 2,329,861 + +0.3 2,329,861 + -1.3 2,329,861 + +1.4 2,329,861 + +0.9 2,329,861 + +0.5 2,329,861 + +0.8 2,329,861 + +1.2 2,329,861 + +0.6 2,329,869 - +0.3 2,329,869 - +0.5 2,329,869 - +0.1 2,329,869 - +1.2 2,329,869 - +1.9 2,329,869 - +0.5 2,329,869 - -0.3 2,329,927 - -0.4 2,329,957 - Synpcc7942_2262 0.12 -0.7 2,329,959 + Synpcc7942_2262 0.12 +1.2 2,329,970 + Synpcc7942_2262 0.13 +0.4 2,329,973 - Synpcc7942_2262 0.13 -0.4 2,329,978 + Synpcc7942_2262 0.14 +1.2 2,330,011 + +2.4 2,330,019 - Synpcc7942_2262 0.16 +0.4 2,330,019 - Synpcc7942_2262 0.16 +1.2 2,330,043 - Synpcc7942_2262 0.18 +1.0 2,330,054 - Synpcc7942_2262 0.19 +2.6 2,330,062 - Synpcc7942_2262 0.20 -1.5 2,330,092 + Synpcc7942_2262 0.22 +0.3 2,330,099 + Synpcc7942_2262 0.22 -0.0 2,330,146 + Synpcc7942_2262 0.25 +0.9 2,330,148 - Synpcc7942_2262 0.26 -1.5 2,330,154 - Synpcc7942_2262 0.26 +0.2 2,330,179 - +2.6 2,330,204 - Synpcc7942_2262 0.30 +1.9
Or see this region's nucleotide sequence