Experiment: BG11 with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysM and Synpcc7942_0172 overlap by 4 nucleotides Synpcc7942_0172 and cysQ overlap by 4 nucleotides cysQ and Synpcc7942_0174 are separated by 39 nucleotides Synpcc7942_0174 and Synpcc7942_0175 overlap by 4 nucleotides
Synpcc7942_0171: cysM - cysteine synthase A, at 170,797 to 171,915
cysM
Synpcc7942_0172: Synpcc7942_0172 - hypothetical protein, at 171,912 to 172,205
_0172
Synpcc7942_0173: cysQ - 3'-Phosphoadenosine 5'-phosphosulfate (PAPS) 3'-phosphatase-like, at 172,202 to 173,071
cysQ
Synpcc7942_0174: Synpcc7942_0174 - hypothetical protein, at 173,111 to 173,989
_0174
Synpcc7942_0175: Synpcc7942_0175 - hypothetical protein, at 173,986 to 174,198
_0175
Position (kb) 172
173
174 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 171.228 kb on + strand, within cysM at 171.244 kb on + strand, within cysM at 171.251 kb on + strand, within cysM at 171.274 kb on + strand, within cysM at 171.285 kb on + strand, within cysM at 171.293 kb on - strand, within cysM at 171.337 kb on + strand, within cysM at 171.361 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand, within cysM at 171.515 kb on + strand at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.523 kb on - strand, within cysM at 171.559 kb on + strand at 171.559 kb on + strand, within cysM at 171.567 kb on - strand, within cysM at 171.567 kb on - strand, within cysM at 171.627 kb on - strand, within cysM at 171.687 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.694 kb on + strand, within cysM at 171.695 kb on - strand, within cysM at 171.707 kb on + strand, within cysM at 171.713 kb on + strand, within cysM at 171.725 kb on + strand, within cysM at 171.746 kb on + strand, within cysM at 171.858 kb on + strand at 171.866 kb on - strand at 171.874 kb on - strand at 171.884 kb on - strand at 171.959 kb on + strand, within Synpcc7942_0172 at 171.959 kb on - strand, within Synpcc7942_0172 at 171.965 kb on - strand, within Synpcc7942_0172 at 171.969 kb on + strand, within Synpcc7942_0172 at 172.008 kb on - strand, within Synpcc7942_0172 at 172.061 kb on - strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.101 kb on + strand, within Synpcc7942_0172 at 172.118 kb on - strand, within Synpcc7942_0172 at 172.204 kb on + strand at 172.212 kb on - strand at 172.222 kb on - strand at 172.239 kb on - strand at 172.243 kb on + strand at 172.296 kb on - strand, within cysQ at 172.310 kb on - strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on + strand, within cysQ at 172.323 kb on - strand, within cysQ at 172.334 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.360 kb on + strand, within cysQ at 172.368 kb on - strand, within cysQ at 172.406 kb on + strand, within cysQ at 172.426 kb on - strand, within cysQ at 172.438 kb on + strand, within cysQ at 172.472 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.477 kb on + strand, within cysQ at 172.485 kb on - strand, within cysQ at 172.612 kb on - strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand, within cysQ at 172.625 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand at 172.631 kb on + strand, within cysQ at 172.631 kb on + strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.639 kb on - strand, within cysQ at 172.666 kb on + strand, within cysQ at 172.698 kb on + strand, within cysQ at 172.704 kb on - strand, within cysQ at 172.733 kb on + strand, within cysQ at 172.776 kb on + strand, within cysQ at 172.808 kb on - strand, within cysQ at 172.813 kb on - strand, within cysQ at 172.813 kb on - strand at 172.815 kb on + strand, within cysQ at 172.830 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.953 kb on + strand, within cysQ at 172.961 kb on - strand, within cysQ at 172.961 kb on - strand, within cysQ at 172.961 kb on - strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.968 kb on + strand, within cysQ at 172.973 kb on + strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.976 kb on - strand, within cysQ at 172.989 kb on - strand at 173.016 kb on - strand at 173.039 kb on - strand at 173.062 kb on - strand at 173.062 kb on - strand at 173.066 kb on + strand at 173.071 kb on - strand at 173.075 kb on + strand at 173.075 kb on + strand at 173.075 kb on + strand at 173.083 kb on - strand at 173.084 kb on - strand at 173.084 kb on - strand at 173.101 kb on + strand at 173.102 kb on - strand at 173.109 kb on - strand at 173.109 kb on - strand at 173.109 kb on - strand at 173.109 kb on - strand at 173.118 kb on + strand at 173.119 kb on + strand at 173.132 kb on - strand at 173.134 kb on + strand at 173.164 kb on + strand at 173.172 kb on - strand at 173.205 kb on + strand, within Synpcc7942_0174 at 173.205 kb on + strand, within Synpcc7942_0174 at 173.205 kb on + strand, within Synpcc7942_0174 at 173.213 kb on - strand, within Synpcc7942_0174 at 173.222 kb on + strand, within Synpcc7942_0174 at 173.259 kb on - strand, within Synpcc7942_0174 at 173.275 kb on + strand, within Synpcc7942_0174 at 173.275 kb on + strand, within Synpcc7942_0174 at 173.301 kb on + strand, within Synpcc7942_0174 at 173.301 kb on + strand, within Synpcc7942_0174 at 173.301 kb on + strand, within Synpcc7942_0174 at 173.309 kb on - strand, within Synpcc7942_0174 at 173.309 kb on - strand, within Synpcc7942_0174 at 173.311 kb on - strand, within Synpcc7942_0174 at 173.311 kb on - strand, within Synpcc7942_0174 at 173.316 kb on + strand, within Synpcc7942_0174 at 173.319 kb on - strand, within Synpcc7942_0174 at 173.324 kb on - strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.345 kb on + strand, within Synpcc7942_0174 at 173.353 kb on - strand, within Synpcc7942_0174 at 173.353 kb on - strand, within Synpcc7942_0174 at 173.353 kb on - strand, within Synpcc7942_0174 at 173.357 kb on + strand, within Synpcc7942_0174 at 173.357 kb on + strand, within Synpcc7942_0174 at 173.370 kb on - strand, within Synpcc7942_0174 at 173.432 kb on + strand, within Synpcc7942_0174 at 173.433 kb on + strand, within Synpcc7942_0174 at 173.441 kb on - strand, within Synpcc7942_0174 at 173.441 kb on - strand, within Synpcc7942_0174 at 173.467 kb on + strand, within Synpcc7942_0174 at 173.472 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.479 kb on + strand, within Synpcc7942_0174 at 173.487 kb on - strand, within Synpcc7942_0174 at 173.487 kb on - strand, within Synpcc7942_0174 at 173.495 kb on - strand, within Synpcc7942_0174 at 173.495 kb on - strand, within Synpcc7942_0174 at 173.534 kb on + strand, within Synpcc7942_0174 at 173.593 kb on + strand, within Synpcc7942_0174 at 173.597 kb on + strand, within Synpcc7942_0174 at 173.610 kb on + strand, within Synpcc7942_0174 at 173.628 kb on + strand, within Synpcc7942_0174 at 173.628 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.672 kb on + strand, within Synpcc7942_0174 at 173.680 kb on - strand, within Synpcc7942_0174 at 173.715 kb on + strand, within Synpcc7942_0174 at 173.790 kb on + strand, within Synpcc7942_0174 at 173.790 kb on + strand, within Synpcc7942_0174 at 173.807 kb on + strand, within Synpcc7942_0174 at 173.813 kb on - strand, within Synpcc7942_0174 at 173.825 kb on - strand, within Synpcc7942_0174 at 173.846 kb on - strand, within Synpcc7942_0174 at 173.871 kb on + strand, within Synpcc7942_0174 at 173.873 kb on + strand, within Synpcc7942_0174 at 173.905 kb on - strand at 173.960 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrate 200 mM remove 171,228 + cysM Synpcc7942_0171 0.39 -1.5 171,244 + cysM Synpcc7942_0171 0.40 -1.5 171,251 + cysM Synpcc7942_0171 0.41 -2.0 171,274 + cysM Synpcc7942_0171 0.43 +0.0 171,285 + cysM Synpcc7942_0171 0.44 -1.0 171,293 - cysM Synpcc7942_0171 0.44 -1.8 171,337 + cysM Synpcc7942_0171 0.48 -1.0 171,361 + cysM Synpcc7942_0171 0.50 -1.5 171,515 + cysM Synpcc7942_0171 0.64 -1.7 171,515 + cysM Synpcc7942_0171 0.64 -1.7 171,515 + cysM Synpcc7942_0171 0.64 -0.6 171,515 + -0.9 171,523 - cysM Synpcc7942_0171 0.65 -1.0 171,523 - cysM Synpcc7942_0171 0.65 +1.3 171,523 - cysM Synpcc7942_0171 0.65 -2.4 171,523 - cysM Synpcc7942_0171 0.65 -1.7 171,559 + +1.6 171,559 + cysM Synpcc7942_0171 0.68 -0.9 171,567 - cysM Synpcc7942_0171 0.69 -1.0 171,567 - cysM Synpcc7942_0171 0.69 -2.5 171,627 - cysM Synpcc7942_0171 0.74 +0.3 171,687 + cysM Synpcc7942_0171 0.80 -1.5 171,694 + cysM Synpcc7942_0171 0.80 -0.2 171,694 + cysM Synpcc7942_0171 0.80 -1.9 171,695 - cysM Synpcc7942_0171 0.80 -1.8 171,707 + cysM Synpcc7942_0171 0.81 -1.5 171,713 + cysM Synpcc7942_0171 0.82 -2.7 171,725 + cysM Synpcc7942_0171 0.83 -0.0 171,746 + cysM Synpcc7942_0171 0.85 -0.3 171,858 + +0.9 171,866 - -0.5 171,874 - -1.8 171,884 - -2.4 171,959 + Synpcc7942_0172 0.16 +1.8 171,959 - Synpcc7942_0172 0.16 +0.2 171,965 - Synpcc7942_0172 0.18 -2.2 171,969 + Synpcc7942_0172 0.19 -1.0 172,008 - Synpcc7942_0172 0.33 +1.4 172,061 - Synpcc7942_0172 0.51 +1.5 172,101 + Synpcc7942_0172 0.64 +2.6 172,101 + Synpcc7942_0172 0.64 -1.5 172,101 + Synpcc7942_0172 0.64 -3.1 172,118 - Synpcc7942_0172 0.70 +1.2 172,204 + +1.4 172,212 - +0.6 172,222 - +1.6 172,239 - -0.5 172,243 + +1.5 172,296 - cysQ Synpcc7942_0173 0.11 -0.9 172,310 - cysQ Synpcc7942_0173 0.12 +1.4 172,323 + cysQ Synpcc7942_0173 0.14 -0.0 172,323 + cysQ Synpcc7942_0173 0.14 +0.9 172,323 - cysQ Synpcc7942_0173 0.14 -1.3 172,334 + cysQ Synpcc7942_0173 0.15 -1.7 172,360 + cysQ Synpcc7942_0173 0.18 -0.2 172,360 + cysQ Synpcc7942_0173 0.18 -0.5 172,368 - cysQ Synpcc7942_0173 0.19 -1.3 172,406 + cysQ Synpcc7942_0173 0.23 -0.8 172,426 - cysQ Synpcc7942_0173 0.26 +0.5 172,438 + cysQ Synpcc7942_0173 0.27 +0.5 172,472 + cysQ Synpcc7942_0173 0.31 +1.5 172,477 + cysQ Synpcc7942_0173 0.32 +0.8 172,477 + cysQ Synpcc7942_0173 0.32 -0.2 172,485 - cysQ Synpcc7942_0173 0.33 +0.3 172,612 - cysQ Synpcc7942_0173 0.47 +0.8 172,625 + cysQ Synpcc7942_0173 0.49 +1.3 172,625 + cysQ Synpcc7942_0173 0.49 -0.4 172,625 + cysQ Synpcc7942_0173 0.49 +0.7 172,625 + -0.2 172,631 + cysQ Synpcc7942_0173 0.49 +0.6 172,631 + cysQ Synpcc7942_0173 0.49 +0.5 172,631 + cysQ Synpcc7942_0173 0.49 +1.1 172,631 + cysQ Synpcc7942_0173 0.49 +1.1 172,631 + cysQ Synpcc7942_0173 0.49 +0.7 172,631 + cysQ Synpcc7942_0173 0.49 -0.2 172,631 + +0.4 172,631 + cysQ Synpcc7942_0173 0.49 -0.2 172,631 + cysQ Synpcc7942_0173 0.49 +2.4 172,639 - cysQ Synpcc7942_0173 0.50 -0.5 172,639 - cysQ Synpcc7942_0173 0.50 +0.7 172,639 - cysQ Synpcc7942_0173 0.50 +0.6 172,639 - cysQ Synpcc7942_0173 0.50 -0.2 172,639 - cysQ Synpcc7942_0173 0.50 -0.2 172,639 - cysQ Synpcc7942_0173 0.50 -0.9 172,639 - cysQ Synpcc7942_0173 0.50 +0.9 172,639 - cysQ Synpcc7942_0173 0.50 +1.2 172,639 - cysQ Synpcc7942_0173 0.50 -0.0 172,666 + cysQ Synpcc7942_0173 0.53 +0.6 172,698 + cysQ Synpcc7942_0173 0.57 +2.0 172,704 - cysQ Synpcc7942_0173 0.58 -0.5 172,733 + cysQ Synpcc7942_0173 0.61 +0.4 172,776 + cysQ Synpcc7942_0173 0.66 -0.7 172,808 - cysQ Synpcc7942_0173 0.70 -0.1 172,813 - cysQ Synpcc7942_0173 0.70 +0.6 172,813 - +0.4 172,815 + cysQ Synpcc7942_0173 0.70 +0.9 172,830 + cysQ Synpcc7942_0173 0.72 +0.5 172,953 + cysQ Synpcc7942_0173 0.86 -0.6 172,953 + cysQ Synpcc7942_0173 0.86 -0.1 172,953 + cysQ Synpcc7942_0173 0.86 +2.2 172,953 + cysQ Synpcc7942_0173 0.86 +0.9 172,961 - cysQ Synpcc7942_0173 0.87 -0.7 172,961 - cysQ Synpcc7942_0173 0.87 +0.8 172,961 - cysQ Synpcc7942_0173 0.87 +0.5 172,968 + cysQ Synpcc7942_0173 0.88 +1.1 172,968 + cysQ Synpcc7942_0173 0.88 +0.5 172,968 + cysQ Synpcc7942_0173 0.88 +1.1 172,968 + cysQ Synpcc7942_0173 0.88 -0.3 172,968 + cysQ Synpcc7942_0173 0.88 +0.3 172,968 + cysQ Synpcc7942_0173 0.88 +1.0 172,968 + cysQ Synpcc7942_0173 0.88 +0.5 172,973 + cysQ Synpcc7942_0173 0.89 +0.9 172,976 - cysQ Synpcc7942_0173 0.89 -0.3 172,976 - cysQ Synpcc7942_0173 0.89 +0.1 172,976 - cysQ Synpcc7942_0173 0.89 +0.8 172,976 - cysQ Synpcc7942_0173 0.89 +0.1 172,976 - cysQ Synpcc7942_0173 0.89 +0.2 172,989 - +1.2 173,016 - +0.7 173,039 - +0.3 173,062 - +1.4 173,062 - -0.6 173,066 + -0.9 173,071 - +1.0 173,075 + -0.2 173,075 + -0.3 173,075 + -0.3 173,083 - -0.8 173,084 - -0.3 173,084 - -1.6 173,101 + -0.2 173,102 - -0.1 173,109 - -0.0 173,109 - -0.5 173,109 - -0.2 173,109 - +0.3 173,118 + -1.5 173,119 + -0.6 173,132 - -1.1 173,134 + -3.5 173,164 + -3.2 173,172 - -1.5 173,205 + Synpcc7942_0174 0.11 -0.9 173,205 + Synpcc7942_0174 0.11 -1.1 173,205 + Synpcc7942_0174 0.11 -2.0 173,213 - Synpcc7942_0174 0.12 -0.3 173,222 + Synpcc7942_0174 0.13 -2.8 173,259 - Synpcc7942_0174 0.17 -2.9 173,275 + Synpcc7942_0174 0.19 -3.4 173,275 + Synpcc7942_0174 0.19 -0.9 173,301 + Synpcc7942_0174 0.22 -1.7 173,301 + Synpcc7942_0174 0.22 -0.9 173,301 + Synpcc7942_0174 0.22 -1.5 173,309 - Synpcc7942_0174 0.23 -2.9 173,309 - Synpcc7942_0174 0.23 -0.7 173,311 - Synpcc7942_0174 0.23 -0.3 173,311 - Synpcc7942_0174 0.23 -2.2 173,316 + Synpcc7942_0174 0.23 -1.7 173,319 - Synpcc7942_0174 0.24 -3.2 173,324 - Synpcc7942_0174 0.24 -0.8 173,345 + Synpcc7942_0174 0.27 -3.5 173,345 + Synpcc7942_0174 0.27 -3.9 173,345 + Synpcc7942_0174 0.27 -1.6 173,345 + Synpcc7942_0174 0.27 -1.5 173,353 - Synpcc7942_0174 0.28 -1.4 173,353 - Synpcc7942_0174 0.28 -1.8 173,353 - Synpcc7942_0174 0.28 -2.2 173,357 + Synpcc7942_0174 0.28 -2.5 173,357 + Synpcc7942_0174 0.28 -0.2 173,370 - Synpcc7942_0174 0.29 -2.4 173,432 + Synpcc7942_0174 0.37 -1.2 173,433 + Synpcc7942_0174 0.37 -2.0 173,441 - Synpcc7942_0174 0.38 -1.2 173,441 - Synpcc7942_0174 0.38 -1.0 173,467 + Synpcc7942_0174 0.41 -1.7 173,472 + Synpcc7942_0174 0.41 -1.9 173,479 + Synpcc7942_0174 0.42 -1.2 173,479 + Synpcc7942_0174 0.42 -3.9 173,479 + Synpcc7942_0174 0.42 -2.3 173,479 + Synpcc7942_0174 0.42 -0.0 173,479 + Synpcc7942_0174 0.42 +0.2 173,479 + Synpcc7942_0174 0.42 -0.5 173,487 - Synpcc7942_0174 0.43 -2.0 173,487 - Synpcc7942_0174 0.43 -0.6 173,495 - Synpcc7942_0174 0.44 -1.5 173,495 - Synpcc7942_0174 0.44 -0.8 173,534 + Synpcc7942_0174 0.48 -1.2 173,593 + Synpcc7942_0174 0.55 -0.4 173,597 + Synpcc7942_0174 0.55 -1.7 173,610 + Synpcc7942_0174 0.57 -0.9 173,628 + Synpcc7942_0174 0.59 -2.2 173,628 + Synpcc7942_0174 0.59 -0.7 173,672 + Synpcc7942_0174 0.64 -0.6 173,672 + Synpcc7942_0174 0.64 -0.7 173,672 + Synpcc7942_0174 0.64 +0.9 173,672 + Synpcc7942_0174 0.64 -1.5 173,680 - Synpcc7942_0174 0.65 -1.2 173,715 + Synpcc7942_0174 0.69 -0.9 173,790 + Synpcc7942_0174 0.77 -4.0 173,790 + Synpcc7942_0174 0.77 -1.2 173,807 + Synpcc7942_0174 0.79 -2.5 173,813 - Synpcc7942_0174 0.80 -0.2 173,825 - Synpcc7942_0174 0.81 +0.1 173,846 - Synpcc7942_0174 0.84 +0.3 173,871 + Synpcc7942_0174 0.86 -1.5 173,873 + Synpcc7942_0174 0.87 -1.3 173,905 - -0.7 173,960 + -2.6
Or see this region's nucleotide sequence