Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS14035

Experiment: Plant=Bonny_Best_Tomato; Treatment=2dpi_Stem_Attached; Sample=Stem; Collection=Centrifuged_to_remove_stem_sap_homogenized_filtered_pelleted

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS14030 and RS_RS14035 overlap by 8 nucleotidesRS_RS14035 and RS_RS14040 are separated by 26 nucleotides RS_RS14030: RS_RS14030 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 3,018,877 to 3,019,479 _RS14030 RS_RS14035: RS_RS14035 - sigma-54-dependent Fis family transcriptional regulator, at 3,019,472 to 3,021,154 _RS14035 RS_RS14040: RS_RS14040 - two-component sensor histidine kinase, at 3,021,181 to 3,023,229 _RS14040 Position (kb) 3019 3020 3021 3022Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3019.794 kb on + strand, within RS_RS14035at 3020.083 kb on - strand, within RS_RS14035at 3020.100 kb on - strand, within RS_RS14035at 3020.242 kb on - strand, within RS_RS14035at 3020.838 kb on + strand, within RS_RS14035at 3021.356 kb on + strandat 3021.356 kb on + strandat 3021.357 kb on - strandat 3021.357 kb on - strandat 3021.357 kb on - strandat 3021.357 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Bonny_Best_Tomato; Treatment=2dpi_Stem_Attached; Sample=Stem; Collection=Centrifuged_to_remove_stem_sap_homogenized_filtered_pelleted
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3,019,794 + RS_RS14035 0.19 +0.4
3,020,083 - RS_RS14035 0.36 +1.2
3,020,100 - RS_RS14035 0.37 -0.1
3,020,242 - RS_RS14035 0.46 -3.1
3,020,838 + RS_RS14035 0.81 +3.1
3,021,356 + +3.2
3,021,356 + -4.0
3,021,357 - -0.8
3,021,357 - -2.5
3,021,357 - -1.4
3,021,357 - -2.4

Or see this region's nucleotide sequence