Experiment: butyrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03253 and H281DRAFT_03254 are separated by 42 nucleotides H281DRAFT_03254 and H281DRAFT_03255 are separated by 20 nucleotides H281DRAFT_03255 and H281DRAFT_03256 are separated by 62 nucleotides
H281DRAFT_03253: H281DRAFT_03253 - 2-oxohept-3-enedioate hydratase, at 309,841 to 310,644
_03253
H281DRAFT_03254: H281DRAFT_03254 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 310,687 to 311,088
_03254
H281DRAFT_03255: H281DRAFT_03255 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 311,109 to 311,972
_03255
H281DRAFT_03256: H281DRAFT_03256 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 312,035 to 313,492
_03256
Position (kb) 311
312 Strain fitness (log2 ratio) -2
-1
0
1 at 310.161 kb on + strand, within H281DRAFT_03253 at 310.161 kb on + strand, within H281DRAFT_03253 at 310.170 kb on - strand, within H281DRAFT_03253 at 310.381 kb on - strand, within H281DRAFT_03253 at 310.381 kb on - strand, within H281DRAFT_03253 at 311.077 kb on + strand at 311.174 kb on - strand at 311.203 kb on + strand, within H281DRAFT_03255 at 311.574 kb on - strand, within H281DRAFT_03255 at 311.575 kb on + strand, within H281DRAFT_03255 at 311.704 kb on + strand, within H281DRAFT_03255 at 312.376 kb on + strand, within H281DRAFT_03256 at 312.505 kb on - strand, within H281DRAFT_03256
Per-strain Table
Position Strand Gene LocusTag Fraction butyrate (C) remove 310,161 + H281DRAFT_03253 0.40 -1.3 310,161 + H281DRAFT_03253 0.40 +0.0 310,170 - H281DRAFT_03253 0.41 +0.3 310,381 - H281DRAFT_03253 0.67 +0.2 310,381 - H281DRAFT_03253 0.67 +0.3 311,077 + +0.2 311,174 - +0.2 311,203 + H281DRAFT_03255 0.11 +0.8 311,574 - H281DRAFT_03255 0.54 +0.2 311,575 + H281DRAFT_03255 0.54 -0.9 311,704 + H281DRAFT_03255 0.69 +0.9 312,376 + H281DRAFT_03256 0.23 +0.4 312,505 - H281DRAFT_03256 0.32 -2.2
Or see this region's nucleotide sequence