Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27195 and BWI76_RS27200 are separated by 49 nucleotides BWI76_RS27200 and BWI76_RS27205 are separated by 105 nucleotides BWI76_RS27205 and BWI76_RS27210 are separated by 80 nucleotides
BWI76_RS27195: BWI76_RS27195 - alpha-hydroxy-acid oxidizing enzyme, at 5,645,418 to 5,646,648
_RS27195
BWI76_RS27200: BWI76_RS27200 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 5,646,698 to 5,647,171
_RS27200
BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098
_RS27205
BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195
_RS27210
Position (kb) 5647
5648
5649 Strain fitness (log2 ratio) -1
0
1
2 at 5646.406 kb on + strand, within BWI76_RS27195 at 5646.651 kb on - strand at 5646.652 kb on - strand at 5646.866 kb on + strand, within BWI76_RS27200 at 5646.924 kb on - strand, within BWI76_RS27200 at 5646.966 kb on + strand, within BWI76_RS27200 at 5647.005 kb on + strand, within BWI76_RS27200 at 5647.322 kb on + strand at 5648.103 kb on - strand at 5648.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 5,646,406 + BWI76_RS27195 0.80 -0.5 5,646,651 - +0.4 5,646,652 - +0.5 5,646,866 + BWI76_RS27200 0.35 +0.2 5,646,924 - BWI76_RS27200 0.48 -0.8 5,646,966 + BWI76_RS27200 0.57 +2.1 5,647,005 + BWI76_RS27200 0.65 +0.3 5,647,322 + -0.3 5,648,103 - +0.1 5,648,103 - +1.1
Or see this region's nucleotide sequence