Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23025 and BWI76_RS23030 are separated by 1 nucleotides BWI76_RS23030 and BWI76_RS23035 overlap by 4 nucleotides BWI76_RS23035 and BWI76_RS23040 overlap by 4 nucleotides BWI76_RS23040 and BWI76_RS23045 are separated by 10 nucleotides
BWI76_RS23025: BWI76_RS23025 - cobalt ABC transporter substrate-binding protein CbiN, at 4,803,523 to 4,803,804
_RS23025
BWI76_RS23030: BWI76_RS23030 - cobalamin biosynthesis protein CbiM, at 4,803,806 to 4,804,543
_RS23030
BWI76_RS23035: BWI76_RS23035 - precorrin-2 C(20)-methyltransferase, at 4,804,540 to 4,805,253
_RS23035
BWI76_RS23040: BWI76_RS23040 - sirohydrochlorin cobaltochelatase, at 4,805,250 to 4,806,044
_RS23040
BWI76_RS23045: BWI76_RS23045 - cobalt-precorrin-6A/precorrin-6x reductase, at 4,806,055 to 4,806,834
_RS23045
Position (kb) 4804
4805
4806 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4803.606 kb on - strand, within BWI76_RS23025 at 4803.768 kb on - strand, within BWI76_RS23025 at 4804.086 kb on - strand, within BWI76_RS23030 at 4804.305 kb on + strand, within BWI76_RS23030 at 4804.429 kb on + strand at 4804.681 kb on - strand, within BWI76_RS23035 at 4804.823 kb on + strand, within BWI76_RS23035 at 4804.904 kb on - strand, within BWI76_RS23035 at 4804.994 kb on + strand, within BWI76_RS23035 at 4805.015 kb on + strand, within BWI76_RS23035 at 4805.376 kb on - strand, within BWI76_RS23040 at 4805.517 kb on + strand, within BWI76_RS23040 at 4805.547 kb on + strand, within BWI76_RS23040 at 4805.728 kb on - strand, within BWI76_RS23040 at 4805.730 kb on + strand, within BWI76_RS23040 at 4805.756 kb on - strand, within BWI76_RS23040 at 4805.863 kb on + strand, within BWI76_RS23040 at 4805.888 kb on + strand, within BWI76_RS23040 at 4806.162 kb on + strand, within BWI76_RS23045
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 4,803,606 - BWI76_RS23025 0.29 +1.2 4,803,768 - BWI76_RS23025 0.87 +0.0 4,804,086 - BWI76_RS23030 0.38 +0.8 4,804,305 + BWI76_RS23030 0.68 -1.3 4,804,429 + +1.2 4,804,681 - BWI76_RS23035 0.20 -2.9 4,804,823 + BWI76_RS23035 0.40 -0.7 4,804,904 - BWI76_RS23035 0.51 -0.9 4,804,994 + BWI76_RS23035 0.64 -0.1 4,805,015 + BWI76_RS23035 0.67 -0.8 4,805,376 - BWI76_RS23040 0.16 -1.6 4,805,517 + BWI76_RS23040 0.34 -2.3 4,805,547 + BWI76_RS23040 0.37 -1.3 4,805,728 - BWI76_RS23040 0.60 -1.7 4,805,730 + BWI76_RS23040 0.60 +0.4 4,805,756 - BWI76_RS23040 0.64 +0.9 4,805,863 + BWI76_RS23040 0.77 -0.9 4,805,888 + BWI76_RS23040 0.80 -0.1 4,806,162 + BWI76_RS23045 0.14 -1.5
Or see this region's nucleotide sequence